{"pk":16594,"title":"Prevalence of Bordetella pertussis and Bordetella parapertussis in Samples Submitted for RSV Screening","subtitle":null,"abstract":"Background: The clinical presentation of Bordetella pertussis can overlap with that of respiratory syncytial virus (RSV); however, management differs.\n\n\nHypothesis: First, the prevalence of B. pertussis is less than 2% among patients screened for RSV, and second the prevalence of B. parapertussis is also less than 2% among these patients.\n\n\nMethods: Nasal washings submitted to a clinical laboratory for RSV screening were tested for B. pertussis and B. parapertussis, using species-specific real-time polymerase chain reaction (PCR) assays. These were optimized to target conserved regions within a complement gene and the CarB gene, respectively. A Bordetella spp. genus-specific real-time PCR assay was designed to detect the Bhur gene of B. pertussis, B. parapertussis, and B. bronchiseptica. RSV A and B subtypes were tested by reverse transcription-PCR.\n\n\nResults: Four hundred and eighty-nine clinical samples were tested. There was insufficient material to complete testing for one B. pertussis, 10 RSV subtype A, and four RSV subtype B assays. Bordetella pertussis was detected in 3/488 (0.6%) (95% CI 0.1% to 1.8%), while B. parapertussis was detected in 5/489 (1.0%) (95% CI 0.3% to 2.4%). Dual infection of B. pertussis with RSV and of B. parapertussis with RSV occurred in two and in three cases respectively. RSV was detected by PCR in 127 (26.5%).\n\n\nConclusion: The prevalence of B. pertussis in nasal washings submitted for RSV screening was less than 2%. The prevalence of parapertussis may be higher than 2%. RSV with B. pertussis and RSV with B. parapertussis coinfection do occur.\n\n\n[WestJEM. 2008;9:135-140.]","language":"en","license":{"name":"none","short_name":"none","text":"","url":"http://google.com"},"keywords":[{"word":"Bordetella pertussis"},{"word":"Bordetella parpertussis"},{"word":"Respiratory Syncytial Virus"}],"section":"Article","is_remote":true,"remote_url":"https://escholarship.org/uc/item/1j23x5qb","frozenauthors":[{"first_name":"Paul","middle_name":"","last_name":"Walsh","name_suffix":"","institution":"Department of Emergency Medicine, Kern Medical Center, Bakersfield, CA","department":"None"},{"first_name":"Christina","middle_name":"","last_name":"Lim-Overmeyer","name_suffix":"","institution":"Research and Development Department, Medical Diagnostic Laboratories, Hamilton, NJ","department":"None"},{"first_name":"Lauren","middle_name":"","last_name":"Kimmel","name_suffix":"","institution":"Research and Development Department, Medical Diagnostic Laboratories, Hamilton ,NJ","department":"None"},{"first_name":"Melanie","middle_name":"","last_name":"Feola","name_suffix":"","institution":"Research and Development Department, Medical Diagnostic Laboratories, Hamilton ,NJ","department":"None"},{"first_name":"James","middle_name":"","last_name":"Pusavaat","name_suffix":"","institution":"Department of Pathology, Kern Medical Center, Bakersfield ,CA","department":"None"},{"first_name":"Tuan Anh","middle_name":"","last_name":"Nguyen","name_suffix":"","institution":"Department of Emergency Medicine, Kern Medical Center, Bakersfield ,CA","department":"None"},{"first_name":"Sam","middle_name":"","last_name":"Kuan","name_suffix":"","institution":"Department of Emergency Medicine, Kern Medical Center, Bakersfield CA.","department":"None"},{"first_name":"Kirt","middle_name":"","last_name":"Emery","name_suffix":"","institution":"Kern County Department of Public Health","department":"None"},{"first_name":"Martin","middle_name":"","last_name":"Rosengreen","name_suffix":"","institution":"Department of Emergency Medicine, Kern Medical Center, Bakersfield ,CA","department":"None"},{"first_name":"Eli","middle_name":"","last_name":"Mordechai","name_suffix":"","institution":"Research and Development Department, Medical Diagnostic Laboratories, Hamilton ,NJ","department":"None"},{"first_name":"Martin","middle_name":"E","last_name":"Adelson","name_suffix":"","institution":"Research and Development Department, Medical Diagnostic Laboratories, Hamilton ,NJ","department":"None"}],"date_submitted":"2007-11-12T08:00:00Z","date_accepted":"2007-11-12T08:00:00Z","date_published":"2008-06-23T07:00:00Z","render_galley":null,"galleys":[{"label":"","type":"pdf","path":"https://journalpub.escholarship.org/westjem/article/16594/galley/8394/download/"}]}