{"pk":41250,"title":"Sequencing and annotation of the Wolbachia endosymbiont of Diaphorina citri by the CG-HLB Genome Resources group reveals candidate sources of interaction with the insect host","subtitle":null,"abstract":"The Citrus Greening – Huanglongbing (CG-HLB) Genome Resources group serves as a bioinformatics resource for diverse projects related to the biology of CG-HLB.  A major recent project concerns the generation and annotation of a draft genome sequence for the Wolbachia endosymbiont (wDi) of the\n \nAsian citrus psyllid, of particular interest given the potential for control of psyllid behavior through manipulation of its bacterial endosymbionts.   The Wolbachia draft genome was assembled and contigs aligned using the wPip strain from mosquito, its closest relative among completed Wolbachia genome sequences.  OrthoMCL analysis of the annotated draft genome sequence confirmed the presence of 670 genes common to all sequenced Wolbachia genomes. Candidate host interaction factors include 54 predicted ankyrin proteins hypothesized to play a role in host reproductive manipulation, a Type IV secretion system linked to ankyrin protein export, and a bacterioferritin linked to host iron homeostasis.  Several metabolic capabilities were identified in wDi that are absent from Liberibacter\n.\n FtsZ and Wsp phylogenies indicate that the Wolbachia strain in the Florida \nD. citri\n isolate falls into a sub-clade of supergroup B, distinct from Wolbachia present in Chinese \nD. citri\n isolates, supporting the hypothesis that the \nD. citri\n introduced into Florida did not originate from China. The Wolbachia sequence and annotation can be viewed on the CG-HLB Genome Resources Website (http://citrusgreening.org/), together with the sequences of publically available Liberibacter genomes sequenced to date. Future plans involve development of a searchable Liberibacter diagnostic sequence database using the over 1700 publically available \nCa\n. Liberibacter gene sequences.","language":"en","license":{"name":"Creative Commons Attribution 4.0","short_name":"CC BY 4.0","text":"Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.\n\nNo additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.","url":"https://creativecommons.org/licenses/by/4.0"},"keywords":[],"section":"Abstracts of Presentations at the 3rd International Research Conference on Huanglongbing","is_remote":true,"remote_url":"https://escholarship.org/uc/item/29589688","frozenauthors":[{"first_name":"Surya","middle_name":"","last_name":"Saha","name_suffix":"","institution":"Dept. of Plant Pathology and Plant-Microbe Interactions, Cornell University, Ithaca NY 14853","department":"None"},{"first_name":"Wayne","middle_name":"","last_name":"Hunter","name_suffix":"","institution":"USDA-ARS, 2001 South Rock Road, Fort Pierce, FL 34945","department":"None"},{"first_name":"Magdalen","middle_name":"","last_name":"Lindeberg","name_suffix":"","institution":"Dept. of Plant Pathology and Plant-Microbe Interactions, Cornell University, Ithaca NY 14853","department":"None"}],"date_submitted":"2014-11-25T11:26:28-08:00","date_accepted":"2014-11-25T11:26:28-08:00","date_published":"2013-12-31T16:00:00-08:00","render_galley":null,"galleys":[{"label":"","type":"pdf","path":"https://journalpub.escholarship.org/iocv_journalcitruspathology/article/41250/galley/30849/download/"}]}