{"pk":41346,"title":"Engineering Resistance Against Citrus Disease Using Candidate Genes","subtitle":null,"abstract":"Citrus canker is a devastating disease caused by \nXanthomonas axonopodis \npv\n. citri\n (\nXac\n).  The \nNPR1\n gene plays a pivotal role in systemic acquired resistance (SAR) in Arabidopsis.  We report the isolation and characterization of an \nNPR1\n homolog from citrus, namely \nCitrus NPR1 homolog 1 \n(\nCtNH1\n).  When over-expressed in citrus, \nCtNH1\n confers resistance to \nXac \nand leads to constitutive expression of the pathogenesis-related (\nPR\n) gene \nchitinase 1\n (\nChi1\n), suggesting that \nCtNH1\n is orthologous to \nNPR1\n.  In addition, we recently identified two closely-related citrus genes, named \nXbct31\n and \nXbct32\n.  Database searching and sequence analyses reveals that other plant species, including rice, Arabidopsis, tomato, and \nNicotiana benthamiana\n, contain homologs of these two citrus genes and they share high levels of sequence identity at the amino acid level.  When overexpressed in \nNicotiana benthamiana\n via Agrobacterium infiltration-mediated transient expression, the citrus genes as well as their closely-related homologs from rice and Arabidopsis all trigger (HR)-like cell death hypersensitive response.  Since HR is often associated with resistance (\nR\n) gene-mediated immunity, our data suggests \nXbct3s\n represent a family of evolutionarily conserved defense regulators and could be used to heighten defense against citrus diseases including greening and canker.","language":"en","license":{"name":"Creative Commons Attribution 4.0","short_name":"CC BY 4.0","text":"Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.\n\nNo additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.","url":"https://creativecommons.org/licenses/by/4.0"},"keywords":[],"section":"Abstracts of Presentations at the 3rd International Research Conference on Huanglongbing","is_remote":true,"remote_url":"https://escholarship.org/uc/item/2p34j1fm","frozenauthors":[{"first_name":"Xiuhua","middle_name":"","last_name":"Chen","name_suffix":"","institution":"Department of Plant Pathology, University of Florida, Gainesville, USA","department":"None"},{"first_name":"Jinyoung","middle_name":"Y.","last_name":"Barnaby","name_suffix":"","institution":"Department of Biology, Duke University, Durham, USA","department":"None"},{"first_name":"Xiaoen","middle_name":"","last_name":"Huang","name_suffix":"","institution":"Department of Plant Pathology, University of Florida, Gainesville, USA","department":"None"},{"first_name":"Aswathy","middle_name":"","last_name":"Sreedharan","name_suffix":"","institution":"Citrus Research and Education Center, University of Florida, Lake Alfred, USA","department":"None"},{"first_name":"Vladimir","middle_name":"","last_name":"Orbović","name_suffix":"","institution":"Citrus Research and Education Center, University of Florida, Lake Alfred, USA","department":"None"},{"first_name":"Jude","middle_name":"W.","last_name":"Grosser","name_suffix":"","institution":"Citrus Research and Education Center, University of Florida, Lake Alfred, USA","department":"None"},{"first_name":"Nian","middle_name":"","last_name":"Wang","name_suffix":"","institution":"Citrus Research and Education Center, University of Florida, Lake Alfred, USA","department":"None"},{"first_name":"Xinnian","middle_name":"","last_name":"Dong","name_suffix":"","institution":"Department of Biology, Duke University, Durham, USA","department":"None"},{"first_name":"Wen-Yuan","middle_name":"","last_name":"Song","name_suffix":"","institution":"Department of Plant Pathology, University of Florida, Gainesville, USA","department":"None"}],"date_submitted":"2014-12-19T00:48:08Z","date_accepted":"2014-12-19T00:48:08Z","date_published":"2014-01-01T00:00:00Z","render_galley":null,"galleys":[{"label":"","type":"pdf","path":"https://journalpub.escholarship.org/iocv_journalcitruspathology/article/41346/galley/30945/download/"}]}