{"pk":41398,"title":"Comparison of gene expression changes in susceptible, tolerant and resistant hosts in response to infection with Citrus tristeza virus and huanglongbing","subtitle":null,"abstract":"The pathogens \nCandidatus\n Liberibacter asiaticus (Las) and \nCitrus tristeza virus \n(CTV) are both phloem limited and have significant economic impact on citrus production wherever they are found. Studies of host resistance have indicated that \nPoncirus trifoliata \nhas tolerance or resistance to both pathogens, suggesting that there may be some common factors in the 2 kinds of resistance. We have conducted studies of host gene expression changes that occur in response to infection to gain further insight. Controlled inoculation by grafting infected budwood was used to infect potted greenhouse plants of Cleopatra mandarin (\nCitrus reticulata\n), US-897 (\nC. reticulata\n x \nP. trifoliata\n), and US-942 (C. reticulata x P. trifoliata) with CTV and with Las, the pathogen associated with the disease huanglongbing (HLB). Stem and leaf tissue was collected at 10, 20, and 30 weeks after inoculation, DNA and RNA were extracted and subjected to qPCR and RT-qPCR analysis. Few differences in gene expression were observed between mock-inoculated and CTV-inoculated plants. Differences between mock-inoculated and Las-inoculated plants were most pronounced in susceptible Cleopatra plants and at the later stages of infection. Notable was the higher expression of a gene for miraculin-like protein 2 and other defense-related genes in US-897 and US-942 plants independent of infection. It is hypothesized that tolerance or resistance of US-897 and US-942 is associated with a higher constitutive expression of defense-related or other genes associated with the \nP. trifoliata\n parentage, rather than with induced expression in response to bacterial infection.","language":"en","license":{"name":"Creative Commons Attribution 4.0","short_name":"CC BY 4.0","text":"Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.\n\nNo additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.","url":"https://creativecommons.org/licenses/by/4.0"},"keywords":[{"word":"citrus, Poncirus trifoliata, huanglongbing, Citrus tristeza virus, rootstock, gene expression, US-897, US-942"}],"section":"Articles","is_remote":true,"remote_url":"https://escholarship.org/uc/item/5qt4z9c0","frozenauthors":[{"first_name":"K","middle_name":"D","last_name":"Bowman","name_suffix":"","institution":"USDA, ARS, USHRL, 2001 South Rock Road, Fort Pierce, Florida, 34945, USA","department":"None"},{"first_name":"U","middle_name":"","last_name":"Albrecht","name_suffix":"","institution":"Southwest Florida Research and Education Center, University of Florida / Institute of Food and Agricultural Sciences, 2685 SR 29 N, Immokalee, Florida, 34142, USA","department":"None"}],"date_submitted":"2016-03-23T18:59:49Z","date_accepted":"2016-03-23T18:59:49Z","date_published":"2016-04-06T18:58:55Z","render_galley":null,"galleys":[{"label":"","type":"pdf","path":"https://journalpub.escholarship.org/iocv_journalcitruspathology/article/41398/galley/30996/download/"}]}