{"pk":41445,"title":"Evaluating high-resolution computed tomography to study citrus tristeza virus-induced stem pitting","subtitle":null,"abstract":"Citrus tristeza virus (CTV) is the most important viral pathogen of citrus. CTV-induced stem pitting negatively impacts grapefruit and sweet orange production. The mechanisms of stem pitting development in CTV-infected citrus remain unclear. This study evaluated the utility of high-resolution CT scanning as a tool to study stem pitting in live citrus material. CT scans were used to easily identify pits based on differences in tissue density. Stem pits were also mapped and modelled three-dimensionally along the length of the stem. Nano-CT scanning proved to be a potentially valuable, non-destructive method for stem pitting characterization in citrus.","language":"en","license":{"name":"Creative Commons Attribution 4.0","short_name":"CC BY 4.0","text":"Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.\n\nNo additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.","url":"https://creativecommons.org/licenses/by/4.0"},"keywords":[{"word":"CTV"},{"word":"X-ray tomography"},{"word":"3D-modelling"},{"word":"Nano-CT"},{"word":"Micro-CT"}],"section":"Articles","is_remote":true,"remote_url":"https://escholarship.org/uc/item/4fw3c8pg","frozenauthors":[{"first_name":"Dirk","middle_name":"Jacobus","last_name":"Aldrich","name_suffix":"","institution":"Department of Genetics, Stellenbosch University, Private Bag X1, Matieland 7602, South Africa\n\nAgricultural Research Council, Infruitec-Nietvoorbij: Institute for Deciduous Fruit, Vines and Wine, Private Bag X5026, Stellenbosch 7599, South Africa","department":"None"},{"first_name":"Rachelle","middle_name":"","last_name":"Bester","name_suffix":"","institution":"Department of Genetics, Stellenbosch University, Private Bag X1, Matieland 7602, South Africa","department":"None"},{"first_name":"Glynnis","middle_name":"","last_name":"Cook","name_suffix":"","institution":"Citrus Research International, PO Box 28, Nelspruit 1200, Mpumalanga, South Africa","department":"None"},{"first_name":"Anton","middle_name":"","last_name":"du Plessis","name_suffix":"","institution":"Research group 3D Innovation, Stellenbosch University, Stellenbosch, South Africa","department":"None"},{"first_name":"Johan","middle_name":"Theodorus","last_name":"Burger","name_suffix":"","institution":"Department of Genetics, Stellenbosch University, Private Bag X1, Matieland 7602, South Africa","department":"None"},{"first_name":"Hans","middle_name":"Jacob","last_name":"Maree","name_suffix":"","institution":"Department of Genetics, Stellenbosch University, Private Bag X1, Matieland 7602, South Africa\n\nCitrus Research International, PO Box 2201, Matieland, 7602, South Africa","department":"None"}],"date_submitted":"2020-10-01T16:02:20Z","date_accepted":"2020-10-01T16:02:20Z","date_published":"2021-04-24T07:00:00Z","render_galley":null,"galleys":[{"label":"","type":"pdf","path":"https://journalpub.escholarship.org/iocv_journalcitruspathology/article/41445/galley/31026/download/"}]}