{"pk":59131,"title":"Decelluarization and Reseeding of Porcine Colon with Human CaCo-2 Cells for Whole-Scale Organ Engineering Applications","subtitle":null,"abstract":"A new approach to whole-scale organ engineering is the seeding of stem cells upon a decellularized scaffold of a desired organ. Current efforts seek to use this method for whole-scale organ engineering. Here, we report a novel decellularizationrecellularization approach to engineering a human colonic graft with human enterocytes and xenogeneic scaffold. Porcine colon tissue was decellularized with protein-solubilizing agents and recellularized by incubation with Caco-2. Immunohistochemistry, DNA staining, and SEM were used to study the effects upon the scaffold. Exposure to 2%SDS-0.5%Triton solution for four hours resulted in the best decellularization and preservation of microstructure, while tissues treated with 2%SDS-0.5%Triton and 0.05%Trypsin/EDTA supported the best recellularization. Our method provides a potential strategy for streamlined regeneration of a viable colonic lumen from xenogeneic and allogeneic sources while maintaining colon microstructure and potential for recellularization.","language":"en","license":null,"keywords":[],"section":"Research","is_remote":true,"remote_url":"https://escholarship.org/uc/item/3b0743s3","frozenauthors":[{"first_name":"Julie","middle_name":"","last_name":"Haduong","name_suffix":"","institution":"University of California, Berkeley","department":""},{"first_name":"Rahul","middle_name":"","last_name":"Nayak","name_suffix":"","institution":"University of California, Berkeley","department":""},{"first_name":"Yumi","middle_name":"","last_name":"Suh","name_suffix":"","institution":"University of California, Berkeley","department":""},{"first_name":"Vinay","middle_name":"","last_name":"Viswanadham","name_suffix":"","institution":"University of California, Berkeley","department":""}],"date_submitted":"2014-12-18T08:28:29Z","date_accepted":"2014-12-18T08:28:29Z","date_published":"2014-12-18T08:00:00Z","render_galley":null,"galleys":[{"label":"","type":"pdf","path":"https://journalpub.escholarship.org/our_bsj/article/59131/galley/45152/download/"}]}