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{ "count": 39542, "next": "https://eartharxiv.org/api/articles/?format=api&limit=100&offset=26200", "previous": "https://eartharxiv.org/api/articles/?format=api&limit=100&offset=26000", "results": [ { "pk": 41280, "title": "A grower question: So we are controlling the Asian Citrus Psyllid, but are we doing it well enough?", "subtitle": null, "abstract": "Since Huanglongbing (HLB) was first found in Florida, growers have recognized the importance of controlling the Asian citrus psyllid (ACP) that vectors the disease. ACP management programs have been developed and applied over large acreages, yet in many instances, the incidence of the disease continues to increase. When asked, the growers invariably report that they are controlling ACP and it is obvious that the control programs are reducing the level of ACP in the groves. However there is an outstanding question as to whether the levels of ACP are being reduced enough to limit the spread of HLB. In most cases, growers have not implemented ACP scouting programs so the level of infestation is based on rough grove surveys and feelings. Even if scouting programs are in place, there are no established data-based thresholds to trigger additional applications of pesticides resulting in a “chasing” approach where applications are made after an infestation is found. Southern Gardens has had an ACP and HLB scouting program in place for many years. By comparing the annual ACP levels to resulting HLB infection succeeding years, it may be possible to elucidate thresholds and then design ACP control programs that effectively limit the spread of HLB. Data will be presented for three years of ACP scouting and four years of HLB scouting in an attempt to show the level of control that must be achieved in order to limit the spread of HLB. The bottom line is that control thresholds are very low and the level of ACP control that must be achieved in order to limit spread HLB is much lower than most growers realize.", "language": "en", "license": { "name": "Creative Commons Attribution 4.0", "short_name": "CC BY 4.0", "text": "Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.\n\nNo additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.", "url": "https://creativecommons.org/licenses/by/4.0" }, "keywords": [], "section": "Abstracts of Presentations at the 3rd International Research Conference on Huanglongbing", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/29j5p26z", "frozenauthors": [ { "first_name": "M.", "middle_name": "", "last_name": "Irey", "name_suffix": "", "institution": "United States Sugar Corporation, Clewiston, FL", "department": "None" }, { "first_name": "T.", "middle_name": "", "last_name": "Gast", "name_suffix": "", "institution": "Southern Gardens Citrus, Clewiston, FL", "department": "None" }, { "first_name": "H.", "middle_name": "", "last_name": "Hou", "name_suffix": "", "institution": "United States Sugar Corporation, Clewiston, FL", "department": "None" } ], "date_submitted": "2014-12-16T22:36:29Z", "date_accepted": "2014-12-16T22:36:29Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/iocv_journalcitruspathology/article/41280/galley/30879/download/" } ] }, { "pk": 41326, "title": "Analyses of proteomic expression profiles and nutrient status of citrus plants in response to Huanglongbing", "subtitle": null, "abstract": "Huanglongbing (HLB) is a highly devastating citrus disease and represents a major threat to the citrus industries in US. The etiology of HLB worldwide is associated with three insect-transmissible phloem-limited members of the bacterial group ‘\nCandidatus\n Liberibacter spp.’, prevalently ‘\nCandidatus\n Liberibacter asiaticus’ (Las). All citrus cultivars are susceptible to HLB. To better understand the physiological and molecular processes involved in host responses to Las, proteomic analyses via 2-DE and mass spectrometry as well as ICP spectroscopy analysis were employed to elucidate protein expression profiles in leaves of Las-infected grapefruit and lemon plants at presymptomatic and symptomatic stages of the disease. Las-mediated down-regulation of 56 proteins including those associated with photosynthesis, protein synthesis, and metabolism was correlated with significant reductions in the concentrations of Ca, Mg, Fe, Zn, Mn, and Cu, especially in symptomatic plants. Interestingly, Las-mediated up-regulation of 13 proteins including those associated with pathogen response, redox-homeostasis, and starch anabolism was correlated with an increase in K concentration in pre-symptomatic and symptomatic plants. Since starch synthase requires K for activation, this result highlights a coordinated accumulation of granule-bound starch synthase and K in Las-infected plants. Analysis of host responses to HLB provides new information concerning physiological and biochemical processes of citrus to HLB. Those proteins that are up regulated specifically in response to Las infection could be useful biomarkers to develop a host-based diagnostic tool for early detection of HLB-affected citrus plants.", "language": "en", "license": { "name": "Creative Commons Attribution 4.0", "short_name": "CC BY 4.0", "text": "Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.\n\nNo additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.", "url": "https://creativecommons.org/licenses/by/4.0" }, "keywords": [], "section": "Abstracts of Presentations at the 3rd International Research Conference on Huanglongbing", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/7tk069kj", "frozenauthors": [ { "first_name": "H.", "middle_name": "", "last_name": "Lin", "name_suffix": "", "institution": "USDA-ARS, Parlier, CA, USA", "department": "None" }, { "first_name": "C.", "middle_name": "C.", "last_name": "Nwugo", "name_suffix": "", "institution": "USDA-ARS, Parlier, CA, USA", "department": "None" }, { "first_name": "Y.", "middle_name": "", "last_name": "Duan", "name_suffix": "", "institution": "USDA-ARS, Fort Pierce, FL, USA", "department": "None" } ], "date_submitted": "2014-12-18T19:24:06Z", "date_accepted": "2014-12-18T19:24:06Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/iocv_journalcitruspathology/article/41326/galley/30925/download/" } ] }, { "pk": 41375, "title": "Analysis and evaluation of China-native citrus and citrus related germplasm on their susceptibility to the infestation by Diaphorina citri Kuwayama (Homotera: Psyllidae)", "subtitle": null, "abstract": "Huanglongbing (HLB) is the most devastating disease of citrus worldwide and vectored by the Asian citrus psyllid (ACP), \nDiaphorina citri\n Kuwayama (Hemiptera: Psyllidae). The pathogen associated with HLB maintains uncultural in vitro, and there are few effective options against HLB-affected plants. Identification and deployment of ACP- resistance traits of citrus and citrus related germplasm to suppress ACP populations may be a potential management strategy for the management of HLB. In the present study, the susceptibilities of 71 Chinese citrus genotypes to ACP infestation were evaluated and analyzed in a free-choice situation under field conditions by using the method of systematic clustering and dynamic clustering. The results showed that there was significant difference in susceptibility to ACP infestation among the genotypes of citrus. These genotypes can be graded according to the number of psyllids on the trees. Grade I: highly susceptible with a total of 8 genotypes. Grade II: moderately susceptible with a total of 18 genotypes. Grade III: lower susceptibility with a total of 45 genotypes. The 71 genotypes of citrus are used to be classified into 8 groups according to the Chinese classification system “Citrus Varieties in China” (Chinese Citrus Association). There existed significant differences among the 8 groups. Lemons and Pummelos were highly susceptible hosts to ACP, with no significant difference from \nMurraya panciculata\n L., which was the most suitable host to ACP. Mandarins, Hybrid citrus, Sweet oranges and Tangerines were moderately susceptible hosts. Kumquat and Sour oranges were lower susceptible hosts. Some genotypes of Kumquat, like \nCitrus medurenisi\n var. Variegated Calamondin, \nFortunella hindsii\n var. Chintou, and Sour orange, \nCitrus aurantium\n var. Variegated sour orange, had the lowest susceptiblility to ACP infection. Further experiment will be done to confirm the low ACP- susceptibility of genotypes from Kumquat and Sour orange.", "language": "en", "license": { "name": "Creative Commons Attribution 4.0", "short_name": "CC BY 4.0", "text": "Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.\n\nNo additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.", "url": "https://creativecommons.org/licenses/by/4.0" }, "keywords": [], "section": "Abstracts of Presentations at the 3rd International Research Conference on Huanglongbing", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/75t1m735", "frozenauthors": [ { "first_name": "Hanqing", "middle_name": "", "last_name": "Hu", "name_suffix": "", "institution": "Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China", "department": "None" }, { "first_name": "Chuanqing", "middle_name": "", "last_name": "Ruan", "name_suffix": "", "institution": "Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China", "department": "None" }, { "first_name": "Bo", "middle_name": "", "last_name": "Liu", "name_suffix": "", "institution": "Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China", "department": "None" }, { "first_name": "Zhenquan", "middle_name": "", "last_name": "Wu", "name_suffix": "", "institution": "Fujian Agriculture and Forestry University,Fuzhou, Fujian 350002, China", "department": "None" }, { "first_name": "Tao", "middle_name": "", "last_name": "Li", "name_suffix": "", "institution": "Fujian Agriculture and Forestry University,Fuzhou, Fujian 350002, China", "department": "None" }, { "first_name": "Guocheng", "middle_name": "", "last_name": "Fan", "name_suffix": "", "institution": "", "department": "None" }, { "first_name": "Yongping", "middle_name": "", "last_name": "Duan", "name_suffix": "", "institution": "U.S. Horticultural Research Laboratory (USDA/ARS), Fort Pierce, FL 34945, USA", "department": "None" }, { "first_name": "David", "middle_name": "G.", "last_name": "Hall", "name_suffix": "", "institution": "U.S. Horticultural Research Laboratory (USDA/ARS), Fort Pierce, FL 34945, USA", "department": "None" } ], "date_submitted": "2014-12-23T00:12:48Z", "date_accepted": "2014-12-23T00:12:48Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/iocv_journalcitruspathology/article/41375/galley/30974/download/" } ] }, { "pk": 41271, "title": "Analysis of Methods/Systems for Delivery of Volatile Repellent Compounds to Protect Young Citrus Plantings from HLB", "subtitle": null, "abstract": "Control of Asian citrus psyllids is critical for the citrus industry to survive. Citrus growers urgently need to be provided with new tools using recent technological developments for best control practices. One approach is the use of plant-based or natural volatile repellent compounds. Although there have been significant advances in laboratory studies, there is still no effective repellency system developed for field usage. Most importantly, even if the most effective psyllid repellent is utilized, its field performance may be less than desired if the most effective method of delivery is not employed. A system approach is required – the volatile repellent must be “matched” to the delivery methodology – to achieve the optimal overall repellency system, which also requires a physicochemical and engineering understanding of the repellency system employed to provide the sought-after control strategy. This presentation will examine both wax-based and vapor-based dispensing approaches. The volatile substances released from wax-based formulations are analogous to “contact repellents”, whereas those released from vapor-based systems function as “spatial repellents” – two different approaches for delivery of volatile repellent compounds. We present a break-through in the art of controlled delivery of volatile compounds using a system approach. The Auburn vapor-based invention has many advantages, one of which is the flexibility it offers in the design and engineering of vapor delivery systems for biocontrol strategies in the citrus industry.", "language": "en", "license": { "name": "Creative Commons Attribution 4.0", "short_name": "CC BY 4.0", "text": "Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.\n\nNo additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.", "url": "https://creativecommons.org/licenses/by/4.0" }, "keywords": [], "section": "Abstracts of Presentations at the 3rd International Research Conference on Huanglongbing", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/3sn9d5vj", "frozenauthors": [ { "first_name": "R.", "middle_name": "D.", "last_name": "Neuman", "name_suffix": "", "institution": "Auburn University, Auburn, AL, USA", "department": "None" }, { "first_name": "A.", "middle_name": "B.", "last_name": "Shelton", "name_suffix": "", "institution": "Auburn University, Auburn, AL, USA", "department": "None" }, { "first_name": "R.", "middle_name": "H.", "last_name": "Zee", "name_suffix": "", "institution": "Auburn University, Auburn, AL, USA", "department": "None" } ], "date_submitted": "2014-12-16T22:11:05Z", "date_accepted": "2014-12-16T22:11:05Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/iocv_journalcitruspathology/article/41271/galley/30870/download/" } ] }, { "pk": 59982, "title": "An Audio-Visual Notice Of Use Database: A Solution To The Orphan Works Problem In The Internet Age", "subtitle": null, "abstract": "[no abstract]", "language": "en", "license": { "name": "", "short_name": "", "text": null, "url": "" }, "keywords": [], "section": "Articles", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/7hp0m7fx", "frozenauthors": [ { "first_name": "Erez", "middle_name": "", "last_name": "Rosenberg", "name_suffix": "", "institution": "", "department": "" } ], "date_submitted": "2014-12-19T23:51:16Z", "date_accepted": "2014-12-19T23:51:16Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/uclalaw_elr/article/59982/galley/45940/download/" } ] }, { "pk": 41253, "title": "An Evaluation of Different Plant Species for Rearing Asian Citrus Psyllid, Diaphorina citri Kuwayama (Hemiptera: Psyllidae)", "subtitle": null, "abstract": "Many research projects concerning the Asian citrus psyllid (ACP) are dependent on a steady supply of ACP. ACP is not a difficult insect to rear in most respects, and basic information on rearing procedures has been published (Skelley and Hoy 2004). Skelley and Hoy (2004) reported on rearing procedures using the host plant \nMurraya\n \nexotica\n (=\npaniculata\n) L. USDA-ARS in Fort Pierce, Florida has reared ACP on \nM. exotica\n (Hall et al. 2007) and also on \nCitrus macrophylla\n Wester (Hall and Richardson 2012). ACP only lay eggs on newly emerging flush points, and nymphs only develop on flush – flushing characteristics of some plant species may be better than others for rearing ACP. A plant can be trimmed to stimulate development of flush. Intuitively, the more flush points a plant produces, the greater the ACP production potential.", "language": "en", "license": { "name": "Creative Commons Attribution 4.0", "short_name": "CC BY 4.0", "text": "Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.\n\nNo additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.", "url": "https://creativecommons.org/licenses/by/4.0" }, "keywords": [], "section": "Abstracts of Presentations at the 3rd International Research Conference on Huanglongbing", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/3d77r55p", "frozenauthors": [ { "first_name": "David", "middle_name": "G.", "last_name": "Hall", "name_suffix": "", "institution": "USDA-ARS, 2001 South Rock Road, Fort Pierce, FL 34945", "department": "None" }, { "first_name": "Joseph", "middle_name": "P.", "last_name": "Albano", "name_suffix": "", "institution": "USDA-ARS, 2001 South Rock Road, Fort Pierce, FL 34945", "department": "None" } ], "date_submitted": "2014-11-25T19:32:03Z", "date_accepted": "2014-11-25T19:32:03Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/iocv_journalcitruspathology/article/41253/galley/30852/download/" } ] }, { "pk": 41244, "title": "A new method for spatial analysis of irregularly spaced HLB data and biological implications", "subtitle": null, "abstract": "Field data on intensity of plant diseases is very often irregularly spaced (i.e., there are varying amounts of distance between rows, ponds, voids, roads, houses, or other land areas). Typically, this type of data is gridded and the average disease intensity of the plants within the grid is used instead of the original data on each separate plant. This is done because the underlying statistical assumptions in the analysis of spatial data usually require that data be equally spaced. However, a new method of analysis, sometimes called second-generation wavelet analysis, can be used on irregularly spaced spatial data. Wavelet analysis is a method used to analyze variations in scale and position of non-stationary spatial signals (non-stationary for our data means the statistical properties can vary based on location within the orchard), and the second-generation refers to an iterative process, called a lifting scheme (1), which allows for the irregular spacing. Irregular spacing is often found in citrus groves as spacing within and between rows is often not uniform, and on a larger spatial scale, distance between blocks and plantings are not necessarily simple multiples of distances between rows and trees. In addition, there are a number of other issues such as missing trees, the presence of irregular roads, ponds, staging areas, etc., that cause citrus groves to have irregular distances between trees when viewed at the plantation or regional scale. Therefore, to test this new method, we conducted a second-generation spatial wavelet analysis on a large irregularly spaced citrus planting (Southern Gardens) in Florida where over 260,000 trees were assessed for incidence of huanglongbing (HLB) over five sampling times.", "language": "en", "license": { "name": "Creative Commons Attribution 4.0", "short_name": "CC BY 4.0", "text": "Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.\n\nNo additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.", "url": "https://creativecommons.org/licenses/by/4.0" }, "keywords": [], "section": "Abstracts of Presentations at the 3rd International Research Conference on Huanglongbing", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/4f50v4mc", "frozenauthors": [ { "first_name": "Alissa", "middle_name": "B.", "last_name": "Kriss", "name_suffix": "", "institution": "USDA, ARS, US Horticultural Research Laboratory, Fort Pierce, Florida, USA", "department": "None" }, { "first_name": "Michael", "middle_name": "S.", "last_name": "Irey", "name_suffix": "", "institution": "Southern Gardens Citrus, US Sugar Corp., Clewiston, Florida, USA", "department": "None" }, { "first_name": "Tim", "middle_name": "R.", "last_name": "Gottwald", "name_suffix": "", "institution": "USDA, ARS, US Horticultural Research Laboratory, Fort Pierce, Florida, USA", "department": "None" } ], "date_submitted": "2014-11-25T18:43:35Z", "date_accepted": "2014-11-25T18:43:35Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/iocv_journalcitruspathology/article/41244/galley/30843/download/" } ] }, { "pk": 6024, "title": "An Exploration of the Perceptions and Utilizations of Networking Systems in Washington, D.C.", "subtitle": null, "abstract": "An Exploration of the Perceptions and Utilizations of NetworkingSystems in Washington, D.C.", "language": "en", "license": { "name": "All rights reserved", "short_name": "Copyright", "text": "© the author(s). All rights reserved.", "url": "https://creativecommons.org/licenses/authors" }, "keywords": [], "section": "Articles", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/9sz8c1p0", "frozenauthors": [ { "first_name": "Robynne", "middle_name": "", "last_name": "Lindsey", "name_suffix": "", "institution": "", "department": "None" } ], "date_submitted": "2014-11-23T05:05:22Z", "date_accepted": "2014-11-23T05:05:22Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "", "path": "https://journalpub.escholarship.org/our_buj/article/6024/galley/3662/download/" } ] }, { "pk": 59107, "title": "An Interview with Professor Jan Rabaey: Neural Prosthetics and Their Future Applications", "subtitle": null, "abstract": "", "language": "en", "license": null, "keywords": [], "section": "Interviews", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/3hm816zr", "frozenauthors": [ { "first_name": "Kuntal", "middle_name": "", "last_name": "Chowdhary", "name_suffix": "", "institution": "University of California, Berkeley", "department": "" }, { "first_name": "Jingyan", "middle_name": "", "last_name": "Wang", "name_suffix": "", "institution": "University of California, Berkeley", "department": "" }, { "first_name": "Saavan", "middle_name": "", "last_name": "Patel", "name_suffix": "", "institution": "University of California, Berkeley", "department": "" }, { "first_name": "Shruti", "middle_name": "", "last_name": "Koti", "name_suffix": "", "institution": "University of California, Berkeley", "department": "" } ], "date_submitted": "2014-04-29T02:48:09+01:00", "date_accepted": "2014-04-29T02:48:09+01:00", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/our_bsj/article/59107/galley/45135/download/" } ] }, { "pk": 60224, "title": "\"An Offer California Can't Refuse\": How an Efficient and Adaptable Framework Can Improve Remedies Under the Talent Agency Act and Correct the Issues With its Interpretation", "subtitle": null, "abstract": "California has a longstanding issue with the Talent Agency Act, which\n \nstates that only a licensed agent may seek out, or procure, employment\n \nfor an artist. The TAA has caused major headaches for Hollywood's\n \npersonal managers, who find their contracts with artists voided for engaging\n \nin even minor acts of procurement. Many commentators initially\n \nbelieved that \nMarathon Entertainment Inc. v. Blasi\n solved the dilemma.\n \nHowever, it turns out that the Labor Commissioner, who has\n \nexclusive jurisdiction to hear claims arising under the TAA, continues\n \nto void contracts between California's personal managers and their clients\n \nat an alarming rate. Personal managers disapprove of the Labor\n \nCommissioner's failure to employ the doctrine of severability, as advised\n \nby the Blasi court, to these contracts. In response, the personal\n \nmanagers recently filed a challenge to the constitutionality of the TAA.\n \nThe United States District Court for the Central District of California,\n \nhowever, dismissed the claim and upheld the constitutionality of the\n \ncontroversial Act. Because this debate spans over one hundred years,\n \nand the constitutional challenge was unsuccessful, the authors of this\n \ncomment advocate a two-fold approach to correcting the dilemma: (1)\n \nplace the burden of production in Labor Commissioner hearings on the\n \nartist to prove that the entire manager contract should be voided, and\n \n(2) assess statutory civil penalties to those personal managers who willfully\n \nviolate the TAA by procuring employment. The authors of this\n \nComment argue that the California legislature should consider applying\n \nthis approach because it is not only easily adaptable, but also in line\n \nwith the true purpose of the TAA.", "language": "en", "license": { "name": "", "short_name": "", "text": null, "url": "" }, "keywords": [], "section": "Articles", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/6f89g4q9", "frozenauthors": [ { "first_name": "Keith", "middle_name": "", "last_name": "Warren", "name_suffix": "", "institution": "", "department": "" }, { "first_name": "Ryan", "middle_name": "", "last_name": "Wechsler", "name_suffix": "", "institution": "", "department": "" } ], "date_submitted": "2015-04-25T17:39:34+01:00", "date_accepted": "2015-04-25T17:39:34+01:00", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/uclalaw_elr/article/60224/galley/46183/download/" } ] }, { "pk": 41305, "title": "Antimicrobial Compounds to Combat Citrus Huanglongbing", "subtitle": null, "abstract": "Citrus Huanglongbing (HLB) is associated with the fastidious bacterium,\nCandidatus\n Liberibacter, (Las) that is transmitted by a phloem-feeding insect (Citrus Psyllid). An ideal solution to combat citrus HLB is to completely eliminate the bacteria after a single course of the chemotherapy, either active directly on the bacteria or indirectly through induction of host defense compounds. Twenty-seven antimicrobial compounds were screened to test for\n in vivo \nactivities against HLB bacterium while assessing their phytotoxicity to citrus using the optimized graft-based chemotherapy approach (Zhang et al., 2012). The Las bacterial titers were quantified by qPCR from the leaf samples that were taken at 4-months and 6-months after inoculation, respectively. The Las-infected plants were considered as Las positive with threshold cycle (Ct) values less than 32.0. The efficiency against the HLB bacterium of the tested compound was evaluated by Ct values in the inoculated plants (both scions and rootstocks), scion infected percentage and HLB bacterial transmission percentage. The phytotoxicity was determined by the survival and growth of scions treated by antimicrobial compounds. The clustering results indicated that 27 antimicrobial compounds were divided into 3 groups. The first group including 12 compounds, such as Zineb, was not effective in eliminating the HLB bacteria, with high scion infection (67.9% ± 14.4% on average)， Las transmission percentage (83.4% ± 13.85 on average) as well as the high bacterial titers. The second group of only two compounds was also not effective against the HLB bacterium, but had high phytotoxicity to citrus (less than 40% of the scion survival and 15% of the scion growth). The third group including 13 compounds, such as nicotine, was effective in eliminating the Las bacteria and had no phytotoxicity to citrus. The effective compounds will be further tested in the container-potted plants and in the field.", "language": "en", "license": { "name": "Creative Commons Attribution 4.0", "short_name": "CC BY 4.0", "text": "Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.\n\nNo additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.", "url": "https://creativecommons.org/licenses/by/4.0" }, "keywords": [], "section": "Abstracts of Presentations at the 3rd International Research Conference on Huanglongbing", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/7h32r6jx", "frozenauthors": [ { "first_name": "Muqing", "middle_name": "", "last_name": "Zhang", "name_suffix": "", "institution": "Indian River Research and Education Center, IFAS-UF, Fort Pierce, FL 34945, USA; \nUSDA-ARS, US Horticultural Lab, Fort Pierce, FL34945,USA; \nState Key Lab for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi Univ.,Guangxi 530004", "department": "None" }, { "first_name": "Ying", "middle_name": "", "last_name": "Guo", "name_suffix": "", "institution": "Indian River Research and Education Center, IFAS-UF, Fort Pierce, FL 34945, USA", "department": "None" }, { "first_name": "Charles", "middle_name": "A.", "last_name": "Powell", "name_suffix": "", "institution": "Indian River Research and Education Center, IFAS-UF, Fort Pierce, FL 34945, USA", "department": "None" }, { "first_name": "Yongping", "middle_name": "", "last_name": "Duan", "name_suffix": "", "institution": "Indian River Research and Education Center, IFAS-UF, Fort Pierce, FL 34945, USA", "department": "None" } ], "date_submitted": "2014-12-17T19:54:31Z", "date_accepted": "2014-12-17T19:54:31Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/iocv_journalcitruspathology/article/41305/galley/30904/download/" } ] }, { "pk": 45180, "title": "A Path through the Woods: Remediating Affective Landscapes in Documentary Asylum Worlds", "subtitle": null, "abstract": "In his book \nLandscape and Memory\n, Simon Schama argues that an engagement with the legacies of German nationalism requires a track through the woods: throughout German history, forests have played a key role as origin myth to found a national identity (Schama 1995). In today’s forests, these entanglements between the forest landscape and the nation acquire a strange and unhomely twist: in former East German states, such as Brandenburg and Thuringia, asylum seekers—many of them from Africa—find themselves living on the ruins of abandoned military barracks in the forest. Their isolation in the forest is the outcome of post-unification asylum policies that responded to a heated nation-wide asylum debate and xenophobic attacks against urban refugee shelters in the 1990s, by relocating refugees to remote new ‘homes’ in rural East Germany—often on former military barracks in the forest.\n \nEngaging with \nForst\n, a documentary film produced in collaboration between refugees and European film-makers, this article asks about the possibilities of a mode of analysis that follows Schama’s call to embark on a track through the woods and shed light on contemporary entanglements between landscape, race and nationalism. \nForst\n breaks with documentary—and ethnographic—conventions of creating empathy via the portrayal of individual life stories, or “cases”. Instead, the film situates its story almost entirely in the claustrophobic environment of a gloomy forest in which refugees live. Doing so, it provokes affects and environments in order to invoke the visceral dimensions of borders and nationalism—a method, I argue, that can be mobilized for social inquiry of both the mediation of racialized exclusions in contemporary asylum worlds in Germany—as well as their material and visceral effects.", "language": "en", "license": null, "keywords": [ { "word": "Holzweg, film, documentary film, forests, ethnography, refugees, asylum, Forst, migration, racial exclusion, affect, affective landscapes, landscapes, remediation, remediations, race, ethnicity, vis.." } ], "section": "Articles", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/4qk4p516", "frozenauthors": [ { "first_name": "Bettina", "middle_name": "", "last_name": "Stoetzer", "name_suffix": "", "institution": "The University of Chicago", "department": "None" } ], "date_submitted": "2014-12-17T13:42:39Z", "date_accepted": "2014-12-17T13:42:39Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/transit/article/45180/galley/33970/download/" } ] }, { "pk": 63753, "title": "Appendix A: Publications from 1989 to 2004", "subtitle": null, "abstract": "This is a partial list of publications relevant to the topic of race and multiraciality, specifically books mostly released in English beginning with the foundational scholarship in the 1989 through 2004. The list was compiled by Paul Spickard and Steven F. Riley and edited by Jacqueline Heckman and G. Reginald Daniel.", "language": "en", "license": { "name": "", "short_name": "", "text": null, "url": "" }, "keywords": [ { "word": "racially mixed people" }, { "word": "multiracial identity" }, { "word": "mixed race identity" }, { "word": "mixed race studies" }, { "word": "critical mixed race studies" }, { "word": "multiracial studies" }, { "word": "critical multiracial studies." } ], "section": "Front Matter", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/9p68d37g", "frozenauthors": [ { "first_name": "Steven", "middle_name": "F.", "last_name": "Riley", "name_suffix": "", "institution": "", "department": "" } ], "date_submitted": "2014-01-27T02:36:30Z", "date_accepted": "2014-01-27T02:36:30Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/jcmrs/article/63753/galley/48957/download/" } ] }, { "pk": 63754, "title": "Appendix B: Publications from 2005 to 2013", "subtitle": null, "abstract": "This is a partial list of publications relevant to the topic of race and multiraciality, specifically books, mostly released in English from 2005 to 2013, including some publications that are forthcoming in 2014. The list was compiled by Steven F. Riley and edited by Jacqueline Heckman and G. Reginald Daniel.", "language": "en", "license": { "name": "", "short_name": "", "text": null, "url": "" }, "keywords": [ { "word": "racially mixed people" }, { "word": "multiracial identity" }, { "word": "mixed race identity" }, { "word": "mixed race studies" }, { "word": "critical mixed race studies" }, { "word": "multiracial studies" }, { "word": "critical multiracial studies." } ], "section": "Front Matter", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/6sx1g97g", "frozenauthors": [ { "first_name": "Steven", "middle_name": "F.", "last_name": "Riley", "name_suffix": "", "institution": "", "department": "" } ], "date_submitted": "2014-01-27T02:38:38Z", "date_accepted": "2014-01-27T02:38:38Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/jcmrs/article/63754/galley/48958/download/" } ] }, { "pk": 59100, "title": "Applications of Magnetoelectric Materials for Solid-State Devices", "subtitle": null, "abstract": "", "language": "en", "license": null, "keywords": [], "section": "Features", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/0tp3q8mf", "frozenauthors": [ { "first_name": "Karthik", "middle_name": "", "last_name": "Gururangan", "name_suffix": "", "institution": "University of California, Berkeley", "department": "" } ], "date_submitted": "2014-04-29T02:37:25+01:00", "date_accepted": "2014-04-29T02:37:25+01:00", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/our_bsj/article/59100/galley/45128/download/" } ] }, { "pk": 41372, "title": "A quick evaluation method of AtNPR1 transgenic plants for resistance to HLB", "subtitle": null, "abstract": "We have produced a number of 'Carrizo' citrange (\nCitrus sinensis x Poncirus trifoliata) \ntransformed with the \nArabidopsis thaliana\n NPR1, a transcriptional co-activator that is key in the regulation of systemic acquired resistance (SAR) and the expression of pathogenesis related (PR) genes. Over-expression of this gene has been shown to induce broad spectrum disease resistance in several species. One of the limitations in obtaining genetically resistant citrus plants to HLB is how lengthy it is to propagate and evaluate the transgenic plants. Using grafting with infected budwood takes several months, is labor intensive and normally requires specialized greenhouse space which can be limited. We have developed a system to quickly screen AtNPR1 transgenic lines and determine if they exhibit an enhanced defense response to \nCandidatus\n Liberibacter asiaticus PAMPs. First, we used a synthetic peptide of L-flg22 (22 amino acid flagellin epitope derived from CLas) capable of triggering immunity in citrus. Second, using real time PCR, we determined changes in the expression levels of a battery of genes associated with defense in citrus in a time course of up to 72 hours after infiltration with L-flg22 and compared it with the expression in wild type plants. Certain lines consistently showed an enhanced defense response when exposed to L-flg22 thus identifying the ones with the most potential. The advantage of this method as a first step in the screening process is that is quick, controlled and does not require specialized greenhouse space. The selected lines are being further evaluated through graft inoculation for their tolerance to HLB.", "language": "en", "license": { "name": "Creative Commons Attribution 4.0", "short_name": "CC BY 4.0", "text": "Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.\n\nNo additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.", "url": "https://creativecommons.org/licenses/by/4.0" }, "keywords": [], "section": "Abstracts of Presentations at the 3rd International Research Conference on Huanglongbing", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/8xs0h5d2", "frozenauthors": [ { "first_name": "Vicente", "middle_name": "J.", "last_name": "Febres", "name_suffix": "", "institution": "", "department": "None" }, { "first_name": "Fabiana", "middle_name": "", "last_name": "Rezende-Muniz", "name_suffix": "", "institution": "", "department": "None" }, { "first_name": "Gloria", "middle_name": "A.", "last_name": "Moore", "name_suffix": "", "institution": "", "department": "None" } ], "date_submitted": "2014-12-23T00:02:19Z", "date_accepted": "2014-12-23T00:02:19Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/iocv_journalcitruspathology/article/41372/galley/30971/download/" } ] }, { "pk": 54388, "title": "Are We the 99%? Student Attitudes Toward the Occupy Movements", "subtitle": null, "abstract": "[no abstract available]", "language": "en", "license": { "name": "", "short_name": "", "text": null, "url": "" }, "keywords": [], "section": "Articles", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/3nf5r0f6", "frozenauthors": [ { "first_name": "Tiffany", "middle_name": "", "last_name": "Neman", "name_suffix": "", "institution": "", "department": "" } ], "date_submitted": "2018-05-16T01:28:39+01:00", "date_accepted": "2018-05-16T01:28:39+01:00", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/alephucla/article/54388/galley/41070/download/" } ] }, { "pk": 6025, "title": "Articulating Race, Geography, and Nostalgia in Rural Localities of the Potomac River", "subtitle": null, "abstract": "Articulating Race, Geography, and Nostalgia in Rural Localities ofthe Potomac River", "language": "en", "license": { "name": "All rights reserved", "short_name": "Copyright", "text": "© the author(s). All rights reserved.", "url": "https://creativecommons.org/licenses/authors" }, "keywords": [], "section": "Articles", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/5x77w4fs", "frozenauthors": [ { "first_name": "Gabriel", "middle_name": "", "last_name": "Schwartzman", "name_suffix": "", "institution": "", "department": "None" } ], "date_submitted": "2014-11-23T05:06:38Z", "date_accepted": "2014-11-23T05:06:38Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "", "path": "https://journalpub.escholarship.org/our_buj/article/6025/galley/3663/download/" } ] }, { "pk": 41291, "title": "Asian Citrus Psyllid and Huanglongbing Management in California: How Psyllid Spread Will Affect Grower Costs", "subtitle": null, "abstract": "The spread of Asian Citrus Psyllid (ACP) in California is beginning to reach commercial production. As a vector of the pathogen associated with Huanglongbing (HLB), spread of this insect puts at risk the state's billion dollar citrus industry wherever ACP establishes. Management of ACP and infected tree removal are the only known methods of limiting the spread of HLB, but these methods come with their own set of costs and risks. To reduce economic losses, ACP management options for growers are being developed before ACP and HLB spread throughout commercial citrus growing areas in California. This study will present an ex-ante cost comparison of current pest control practices, the IPM best practices for ACP, and the least cost ACP management program for different citrus growing areas in California. Partial budgeting will be used to estimate the costs under each scenario. Partial budgeting compares the changes in income and expenses that would result from implementing a specific alternative; hence it provides an indicator as to how the treatment is likely to affect the profitability of an enterprise (Kay et al. 2004). Because ACP treatments are applied to reduce the risk of HLB infection and ACP causes far less significant direct damage to citrus, changes in revenues are equal to zero and only costs are calculated. To calculate costs, data on material and application costs were obtained from meetings with pest control advisors in California's citrus growing areas.", "language": "en", "license": { "name": "Creative Commons Attribution 4.0", "short_name": "CC BY 4.0", "text": "Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.\n\nNo additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.", "url": "https://creativecommons.org/licenses/by/4.0" }, "keywords": [], "section": "Abstracts of Presentations at the 3rd International Research Conference on Huanglongbing", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/8d78923p", "frozenauthors": [ { "first_name": "K.", "middle_name": "M.", "last_name": "Jetter", "name_suffix": "", "institution": "University of California Agricultural Issues Center, Davis, USA", "department": "None" }, { "first_name": "E.", "middle_name": "E.", "last_name": "Grafton-Cardwell", "name_suffix": "", "institution": "Dept. of Entomology, University of California Riverside, USA", "department": "None" }, { "first_name": "M.", "middle_name": "P.", "last_name": "Daugherty", "name_suffix": "", "institution": "Dept. of Entomology, University of California Riverside, USA", "department": "None" }, { "first_name": "K.", "middle_name": "", "last_name": "Lynn-Patterson", "name_suffix": "", "institution": "Kearney Agricultural Research and Extension Center, Parlier, USA", "department": "None" } ], "date_submitted": "2014-12-17T00:30:36Z", "date_accepted": "2014-12-17T00:30:36Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/iocv_journalcitruspathology/article/41291/galley/30890/download/" } ] }, { "pk": 41275, "title": "Asian Citrus Psyllid Management Strategies for California, 2012 and Beyond", "subtitle": null, "abstract": "Asian citrus psyllid (ACP) was first detected in southern California in September 2008 in the urban landscape. Since that time, the California Department of Food and Agriculture has conducted an eradication program utilizing systemic imidacloprid and foliar cyfluthrin wherever psyllids are detected. This program was halted in Los Angeles County in 2011 because of the size of the ACP infestation. Psyllids have continued to spread to the east and the south into San Bernardino, Riverside, and Orange Counties. In areas such as San Diego and Imperial counties where urban treatments have been continuous, detections of ACP in commercial citrus are rare and a single treatment of a combination of two broad spectrum insecticides reduces psyllids below detectible levels for many months. In contrast, in San Bernardino and Riverside counties where re-infestation from urban areas is a continuing problem and where the treatments are not well-coordinated in an area-wide fashion, psyllids have become established and commercial growers must treat multiple times per year. The University of California in collaboration with the California Citrus Pest and Disease Prevention Committee have developed strategies for managing ACP once it establishes in commercial citrus. In the initial phase of invasion, when ACP densities are low, aggressive applications of two broad spectrum insecticides are recommended. In areas where the psyllid has become established, area-wide treatment programs utilizing 3-5 insecticides/year are necessary directed at periods of flush and also at the late fall and early spring overwintering populations. Insecticide choices are based on considerations of efficacy, control of other pests, costs, preservation of natural enemies and resistance management.", "language": "en", "license": { "name": "Creative Commons Attribution 4.0", "short_name": "CC BY 4.0", "text": "Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.\n\nNo additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.", "url": "https://creativecommons.org/licenses/by/4.0" }, "keywords": [], "section": "Abstracts of Presentations at the 3rd International Research Conference on Huanglongbing", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/4z57k8km", "frozenauthors": [ { "first_name": "E.", "middle_name": "E.", "last_name": "Grafton-Cardwell", "name_suffix": "", "institution": "University of California, Riverside, CA, USA", "department": "None" }, { "first_name": "J.", "middle_name": "G.", "last_name": "Morse", "name_suffix": "", "institution": "University of California, Riverside, CA, USA", "department": "None" }, { "first_name": "B.", "middle_name": "J.", "last_name": "Taylor", "name_suffix": "", "institution": "Citrus Research Board, Visalia CA, USA", "department": "None" } ], "date_submitted": "2014-12-16T22:24:59Z", "date_accepted": "2014-12-16T22:24:59Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/iocv_journalcitruspathology/article/41275/galley/30874/download/" } ] }, { "pk": 41382, "title": "A Tomato Detached Leaf Assay for Chemical Genomics of an HLB Model System", "subtitle": null, "abstract": "To better understand plant-pathogen interactions in Huanglongbing disease and develop control strategies we investigated a novel approach known as chemical genomics with Tomato “Psyllid Yellows”, caused by \nCandidatus\n Liberibacter psyllaurous (CLps), as a model of HLB. Chemical genomics involves three key stages starting with designing and performing high-throughput chemical screening, identifying chemicals inducing desired effects and dissecting the genetic targets of the candidate chemical. Our study has been focused on developing a high throughput chemical screen assay using model plants such as tomato and Arabidopsis that can be infected by Candidatus \nLiberibacter psyllaurous \n(Clps). The key objective is to identify chemicals that induce plant defense against CLps infection or its transmission via psyllids. We evaluated \nArabidopsis thaliana\n and tomato in different media such as MS sterile media and hydroponic culture, however qPCR results indicated very low and inconsistent numbers of CLps positive plants. We designed a modified detached leaf assay for tomato based on the citrus detached leaf assay (Eldesouky Ammar, USDA-ARS) that has resulted in consistently high (80-85%) number of CLps-positive leaf petioles. We are currently evaluating application of the detached leaf assay in screening chemicals using a tomato CaBP22-GUS transgenic line. This line was developed by Dr. Isgouhi Kaloshian and Dr. Thomas Eulgem (University of California, Riverside) based on a transgenic Arabidopsis reporter line with the promoter \nCaBP22-333\n promoter fused to \nGUS\n (Knoth et.al. 2009). The \nArabidopsis\n transgenic line has been used successfully in many high-throughput chemical screens to identify chemicals inducing defense responses. In our study, we will test chemical uptake and its effect on the transgenic tomato line using GUS expression by RT-PCR. To develop methods to test responses to candidate chemicals in citrus, we are testing gene expression of sweet orange seedlings following exposure to four chemicals known to induce defense responses in other plants.", "language": "en", "license": { "name": "Creative Commons Attribution 4.0", "short_name": "CC BY 4.0", "text": "Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.\n\nNo additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.", "url": "https://creativecommons.org/licenses/by/4.0" }, "keywords": [], "section": "Abstracts of Presentations at the 3rd International Research Conference on Huanglongbing", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/74604423", "frozenauthors": [ { "first_name": "S.", "middle_name": "", "last_name": "Patne", "name_suffix": "", "institution": "Department of Botany and Plant Sciences, University of California, Riverside, CA, USA", "department": "None" }, { "first_name": "T.", "middle_name": "", "last_name": "Eulgem", "name_suffix": "", "institution": "Department of Botany and Plant Sciences, University of California, Riverside, CA, USA", "department": "None" }, { "first_name": "M.", "middle_name": "L.", "last_name": "Roose", "name_suffix": "", "institution": "Department of Botany and Plant Sciences, University of California, Riverside, CA, USA", "department": "None" } ], "date_submitted": "2014-12-23T19:30:11Z", "date_accepted": "2014-12-23T19:30:11Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/iocv_journalcitruspathology/article/41382/galley/30981/download/" } ] }, { "pk": 52620, "title": "Barricades in Berlin: Social Unrest, Constitutionalism, and Revolt in 1848", "subtitle": null, "abstract": "", "language": "en", "license": { "name": "Creative Commons Attribution 4.0", "short_name": "CC BY 4.0", "text": "<p>Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use. No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.</p>", "url": "https://creativecommons.org/licenses/by/4.0" }, "keywords": [ { "word": "History" }, { "word": "Prussia" }, { "word": "social unrest" }, { "word": "Constitutionalism" }, { "word": "Revolt" } ], "section": "Articles", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/9qp4v1bb", "frozenauthors": [ { "first_name": "Josh", "middle_name": "", "last_name": "Teixeira", "name_suffix": "", "institution": "UC Merced", "department": "" } ], "date_submitted": "2014-05-15T19:26:40+01:00", "date_accepted": "2014-05-15T19:26:40+01:00", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/ssha_uhj/article/52620/galley/39676/download/" } ] }, { "pk": 56479, "title": "Barry Gilder, \nSongs & Secrets: South Africa from Liberation to Governance", "subtitle": null, "abstract": "[no abstract]", "language": "en", "license": null, "keywords": [], "section": "Book Review", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/8ph2s97j", "frozenauthors": [ { "first_name": "Nana", "middle_name": "", "last_name": "Osei-Opare", "name_suffix": "", "institution": "UCLA", "department": "" } ], "date_submitted": "2014-12-14T04:19:18Z", "date_accepted": "2014-12-14T04:19:18Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/ufahamu/article/56479/galley/42887/download/" } ] }, { "pk": 41249, "title": "Behavioral, Ultrastructural, and Chemical Studies on the 'Honeydew' Excretions in Nymphs and Adults of the Asian Citrus Psyllid", "subtitle": null, "abstract": "The Asian citrus psyllid (ACP) \nDiaphorina citri\n (Hemiptera: Psyllidae) is the main vector of bacteria responsible for citrus huanglongbing (citrus greening), the most serious citrus disease worldwide. Behavioral and ultrastructural studies on ‘honeydew’ excretions by ACP indicated interesting differences between nymphs, males and females. The anal opening in ACP, near the posterior end of the abdomen, is on the ventral side in nymphs and on the dorsal side in adults. Video recordings showed that males produce clear sticky droplets of honeydew gently laid behind them on the leaf surface, whereas females powerfully expel whitish, different shaped pellets that travel away from the female, probably to get these sticky excretions away from eggs and newly-hatched nymphs. ACP nymphs produce long ribbons or tubes of honeydew excretions that frequently stay attached to the exuviae after molting. Honeydew excretions of both nymphs and adult females are covered with a thin layer of whitish wax-like material ultrastructurally composed of a convoluted network of thin filaments apparently produced by the “wax” glands underneath the anal ring, which is absent in males of this and other psyllid species. The chemical composition of these excretions is being investigated using infrared microscopy and gas chromatography/mass spectroscopy.", "language": "en", "license": { "name": "Creative Commons Attribution 4.0", "short_name": "CC BY 4.0", "text": "Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.\n\nNo additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.", "url": "https://creativecommons.org/licenses/by/4.0" }, "keywords": [], "section": "Abstracts of Presentations at the 3rd International Research Conference on Huanglongbing", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/21m3m60d", "frozenauthors": [ { "first_name": "E.", "middle_name": "D.", "last_name": "Ammar", "name_suffix": "", "institution": "USDA-ARS, 2001 South Rock Road, Fort Pierce, FL 34945", "department": "None" }, { "first_name": "R.", "middle_name": "", "last_name": "Alessandro", "name_suffix": "", "institution": "USDA-ARS, 2001 South Rock Road, Fort Pierce, FL 34945", "department": "None" }, { "first_name": "R.", "middle_name": "G.", "last_name": "Shatters", "name_suffix": "", "institution": "USDA-ARS, 2001 South Rock Road, Fort Pierce, FL 34945", "department": "None" }, { "first_name": "D.", "middle_name": "G.", "last_name": "Hall", "name_suffix": "", "institution": "USDA-ARS, 2001 South Rock Road, Fort Pierce, FL 34945", "department": "None" } ], "date_submitted": "2014-11-25T19:24:58Z", "date_accepted": "2014-11-25T19:24:58Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/iocv_journalcitruspathology/article/41249/galley/30848/download/" } ] }, { "pk": 41325, "title": "Benefit-cost analysis of Huanglongbing management in Sao Paulo, Brazil", "subtitle": null, "abstract": "The Brazilian citrus history is marked by several phytosanitary outbreaks due to entry of new pests, frequently with high potential to generate economic losses. Some of these pests have threatened even the viability of citrus in Sao Paulo State, like Huanglongbing (HLB) that was responsible for eradicating 18 million plants between January 2005 and July 2012. This requires development of new methods to control the disease and ensure the economic viability of the activity. This study aimed to analyze costs and benefits of a broad control of HLB in the state (66 % of orchards are inspected and have HLB-symptomatic trees eradicated) and compare this scenario to another one representing a lower level of control (reference scenario, considering that only 26% of orchards manage HLB and proceed the eradication). We chose the Benefit-Cost Analysis to evaluate the differences between scenarios. Losses caused by HLB comprised a reduction in productivity, elimination of infected plants and increase in production costs (due to inspections, insecticide applications, tree elimination and replanting). Costs and benefits were calculated according to state’s phytosanitary status in 2010. The epidemiological model proposed by Bassanezi & Bassanezi (2008) was used to project the disease progress, crop loss damage, the citrus orchard size, orange production and producers’ costs of production to control the disease over 20 years. The ratio B/C was estimated at 4.07 accumulated for the whole period. This result shows that each R$ 1.00 invested by producers to manage the HLB, prevents them from losing R$ 4.07 in gross revenues.", "language": "en", "license": { "name": "Creative Commons Attribution 4.0", "short_name": "CC BY 4.0", "text": "Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.\n\nNo additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.", "url": "https://creativecommons.org/licenses/by/4.0" }, "keywords": [], "section": "Abstracts of Presentations at the 3rd International Research Conference on Huanglongbing", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/0xg1h7gx", "frozenauthors": [ { "first_name": "A.", "middle_name": "C.O.", "last_name": "Adami", "name_suffix": "", "institution": "Cepea/ESALQ/ University of Sao Paulo, Piracicaba, Brazil", "department": "None" }, { "first_name": "S.", "middle_name": "H.G.", "last_name": "Miranda", "name_suffix": "", "institution": "ESALQ/University of Sao Paulo, Piracicaba, Brazil", "department": "None" }, { "first_name": "R.", "middle_name": "B.", "last_name": "Bassanezi", "name_suffix": "", "institution": "Fundecitrus, Araraquara, Brazil", "department": "None" } ], "date_submitted": "2014-12-17T23:12:40Z", "date_accepted": "2014-12-17T23:12:40Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/iocv_journalcitruspathology/article/41325/galley/30924/download/" } ] }, { "pk": 45186, "title": "BOOK REVIEW: The Counter-Cinema of the Berlin School, by Marco Abel; Christian Petzold, by Jaimey Fisher", "subtitle": null, "abstract": "Review of Marco Abel, \nThe Counter-Cinema of the Berlin School\n (Rochester: Camden House, 2013) and Jaimey Fisher, \nChristian Petzold\n (Urbana, IL: University of Illinois Press, 2013).", "language": "en", "license": null, "keywords": [ { "word": "film, Berlin School, Christian Petzold, realism, Post-Wende, book review" } ], "section": "Book Reviews", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/82x3n1f7", "frozenauthors": [ { "first_name": "Cara", "middle_name": "", "last_name": "Tovey", "name_suffix": "", "institution": "University of California, Berkeley", "department": "None" } ], "date_submitted": "2014-12-17T13:58:07Z", "date_accepted": "2014-12-17T13:58:07Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/transit/article/45186/galley/33976/download/" } ] }, { "pk": 41288, "title": "Brachygastra mellifica (Hymenoptera: Vespidae): Predation preference and feeding behavior on Diaphorina citri (Hemiptera: Psyllidae) in Mexico", "subtitle": null, "abstract": "In previous field studies in Northern Mexico, we found the wasp \nBrachygastra mellifica \n(Say 1837)\n \n(Hymenoptera: Vespidae) preying voraciously the Asian citrus psyllid (ACP), \nDiaphorina citri \nKuwayama\n \n(Hemiptera: Psyllidae), the vector of the bacteria\n Ca. \nLiberibacter spp.,\n \nthe putative agent of Huanglongbing,\n \none of the most devastating citrus disease in the world. As in Mexico, the ACP management considers the use of pest biological control, the availability of potential agents for the control of the vector remains as a priority. The objective of this study was to determine the predation preference of the wasp for the different developmental stages of \nD. citri.\n During 2011-2012, we performed experiments in field where we exposed manually to the predator new citrus flushes infested by eggs and diverse ACP nymphal instars. The predation behavior was recorded in videos and posteriorly analyzed in the lab. Other observations on its feeding attack were made directly on infested flushes in the trees, during the foraging activity of the wasp. Results indicated that \nB. mellifica\n preferred for predation 4th and 5th instar nymphs, and posteriorly 2nd and 3rd instars. Due to their size and position in the flush, eggs and first instar nymphs were practically discriminated by the wasp. Occasionally, \nB. mellifica\n consumed \nD. citri\n adults. This predator represents a potential agent for natural control of \nD. citri\n in the North of Mexico, and South of the USA, mainly through conservation strategies of beneficial insects.", "language": "en", "license": { "name": "Creative Commons Attribution 4.0", "short_name": "CC BY 4.0", "text": "Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.\n\nNo additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.", "url": "https://creativecommons.org/licenses/by/4.0" }, "keywords": [], "section": "Abstracts of Presentations at the 3rd International Research Conference on Huanglongbing", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/6d16q50h", "frozenauthors": [ { "first_name": "M.", "middle_name": "A.", "last_name": "Reyes-Rosas", "name_suffix": "", "institution": "Instituto Nacional Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP), Centro de Investigación Regional Noreste (CIRNE), Campo Experimental Río Bravo, Río Bravo, Tamaulipas, México", "department": "None" }, { "first_name": "J.", "middle_name": "", "last_name": "Loera-Gallardo", "name_suffix": "", "institution": "", "department": "None" }, { "first_name": "J.", "middle_name": "I.", "last_name": "López-Arroyo", "name_suffix": "", "institution": "INIFAP-CIRNE, Campo Experimental General Terán, Nuevo León, México", "department": "None" }, { "first_name": "M.", "middle_name": "", "last_name": "Buck", "name_suffix": "", "institution": "Invertebrate Zoology, Royal Alberta Museum, 12845-102nd Avenue, Edmonton, Alberta, Canada", "department": "None" } ], "date_submitted": "2014-12-17T00:24:49Z", "date_accepted": "2014-12-17T00:24:49Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/iocv_journalcitruspathology/article/41288/galley/30887/download/" } ] }, { "pk": 41379, "title": "Breaking citrus juvenility by modulating endogenous miR156 and miR172 levels", "subtitle": null, "abstract": "The ability to either transform mature citrus directly or to transform juvenile citrus and also induce it to flower and set fruit within a few years is critical for evaluation of fruit quality, quantity and of horticultural performance of any transgenic trees. In plants, the transition from juvenile to adult stage is regulated by the sequential and complementary action of microRNAs miR156 and miR172. miR156 suppresses the expression of specific transcriptional factors that would otherwise promote the juvenile to adult phase transition, including factors that activate miR172, which directly promotes the transition. Here, we created a target mimic for miR156 to attempt to sequester miR156 and reduce its levels in juvenile citrus. We cloned the nonprotein coding gene IPS1 from Arabidopsis and replaced its native microRNA target with the predicted citrus target of citrus miR156, resulting in a citrus miR156 mimic gene, cMIM156. Five sweet orange (Hamlin) seedling transgenic plants expressing cMIM156 were produced and the endogenous miR172 levels were monitored over time. All five transgenic plants showed enhanced expression levels of miR172 (two exhibited 10X higher levels) compared to non-transgenic control plants regenerated from explants at the same time as transformants. By 1 year after transformation, miR172 levels were still 5X less than that observed using mature sweet orange, and no flowering has yet been observed. The levels of miR172 expression have increased over time, and we are attempting to establish a timeline for flowering of 3 years after transformation.", "language": "en", "license": { "name": "Creative Commons Attribution 4.0", "short_name": "CC BY 4.0", "text": "Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.\n\nNo additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.", "url": "https://creativecommons.org/licenses/by/4.0" }, "keywords": [], "section": "Abstracts of Presentations at the 3rd International Research Conference on Huanglongbing", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/27n017qk", "frozenauthors": [ { "first_name": "Y.", "middle_name": "", "last_name": "Jiang", "name_suffix": "", "institution": "Integrated Plant Genetics, Inc., Alachua, FL, USA", "department": "None" }, { "first_name": "D.", "middle_name": "W.", "last_name": "Gabriel", "name_suffix": "", "institution": "Integrated Plant Genetics, Inc., Alachua, FL, USA;\nPlant Pathology Dept., University of Florida, Gainesville, FL, USA", "department": "None" } ], "date_submitted": "2014-12-23T19:24:46Z", "date_accepted": "2014-12-23T19:24:46Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/iocv_journalcitruspathology/article/41379/galley/30978/download/" } ] }, { "pk": 61110, "title": "Breaking Free from Patriarchy: A Comparative Study of Sex Selection Abortions in Korea and the United States", "subtitle": null, "abstract": "", "language": "en", "license": { "name": "", "short_name": "", "text": null, "url": "" }, "keywords": [], "section": "Notes", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/6nr439zx", "frozenauthors": [ { "first_name": "Naryung", "middle_name": "", "last_name": "Kim", "name_suffix": "", "institution": "", "department": "" } ], "date_submitted": "2014-03-30T18:49:42+01:00", "date_accepted": "2014-03-30T18:49:42+01:00", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/uclalaw_pblj/article/61110/galley/47079/download/" } ] }, { "pk": 34751, "title": "BREATHING DIFFERENCE, SHARING EMPOWERMENT", "subtitle": null, "abstract": "We celebrate Margaret E. Montoya’s \nMáscaras, Trenzas, y Greñas\n as a canonical article in critical race theory because its deft interweaving and unbraiding of stories helps us consider the marginalizing assumptions of the legal world, the way normativity translates into authority, and the means by which the mainstream is disguised as unbiased. She does these things effectively through an exercise of courageous candor that lays bare the kind of feelings and thoughts that we usually keep to ourselves or only share with intimates.", "language": "en", "license": { "name": "", "short_name": "", "text": null, "url": "" }, "keywords": [ { "word": "Tehama Lopez Bunyasi, George Mason University, political science, Mascaras, CLLR, Chicana/o-Latina/o Law Review, Latina, Latino, Chicano Studies, Chicano, Margaret Montoya, Race, equality, social ju.." } ], "section": "Articles", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/57n3183d", "frozenauthors": [ { "first_name": "Tehama", "middle_name": "", "last_name": "Lopez Bunyasi", "name_suffix": "", "institution": "", "department": "None" } ], "date_submitted": "2014-05-26T22:13:51+01:00", "date_accepted": "2014-05-26T22:13:51+01:00", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/uclalaw_cllr/article/34751/galley/25895/download/" } ] }, { "pk": 60727, "title": "Breathing Life into a Dormant Statute: Using the Case of the Pink Dolphins to Forge a Path Forward for Environmental Legal Protections in Hong Kong", "subtitle": null, "abstract": "Breathing Life into a Dormant Statute: Using the Case of the Pink Dolphins to Forge a Path Forward for Environmental Legal Protections in Hong Kong", "language": "en", "license": { "name": "", "short_name": "", "text": null, "url": "" }, "keywords": [ { "word": "dolphins, environmental law, hong kong, environmental protection, wildlife, habitat protection, wild animal protection ordinance" } ], "section": "Comments", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/38p412ck", "frozenauthors": [ { "first_name": "Lara", "middle_name": "", "last_name": "Leitner", "name_suffix": "", "institution": "UCLA School of Law, J.D. Candidate, 2014", "department": "" } ], "date_submitted": "2014-05-14T18:36:47+01:00", "date_accepted": "2014-05-14T18:36:47+01:00", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/uclalaw_jelp/article/60727/galley/46691/download/" } ] }, { "pk": 59103, "title": "Bright Ideas in Solar Energy", "subtitle": null, "abstract": "", "language": "en", "license": null, "keywords": [], "section": "Features", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/3zc6w8c2", "frozenauthors": [ { "first_name": "Jo", "middle_name": "", "last_name": "Melville", "name_suffix": "", "institution": "University of California, Berkeley", "department": "" } ], "date_submitted": "2014-04-29T02:41:40+01:00", "date_accepted": "2014-04-29T02:41:40+01:00", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/our_bsj/article/59103/galley/45131/download/" } ] }, { "pk": 41369, "title": "Candidatus Liberibacter americanus induces significant reprogramming of the transcriptome of the susceptible citrus genotype", "subtitle": null, "abstract": "In Brazil, \nHuanglongbing\n (HLB) is caused by \nCandidatus \nLiberibacter americanus (CaLam) and \nCa. \nL. asiaticus (CaLas). Both species are vectored by the Asian citrus psyllid and are restricted to the phloem of infected citrus, where they promote a severe imbalance in the translocation of nutrients and other important substances along the plant. Several studies of the transcriptional response of citrus to HLB have been reported, but only for infection caused by CaLas. This study evaluated the transcriptional reprogramming of a susceptible genotype (Pera sweet orange) challenged with CaLam, using a customized 385K microarray chip. The analyses showed that a large number of genes and biological processes were significantly altered upon CaLam infection. Among the changes we highlight induction of zinc transporters, modulation of enzymes related to sugar metabolism, decreased photosynthesis, induction of several defense-related genes and modulation of enzymes regulating ROS production. Several biological processes reported as differentially modulated upon infection with CaLas responded similarly to CaLam. The large number of receptor-like proteins, PR genes, NBS-LRR and transcription factors (such as WRKY and MYB) found showed that even a susceptible citrus genotype is able to actively respond to infection by CaLam, as reported for CaLas. Twenty candidate genes were selected for validation in symptomatic and asymptomatic PCR-positive leaves of Hamlin sweet orange infected with CaLas or CaLam. Finally, using \nin\n \nsilico\n approaches, we compared our results with all published studies using CaLas to hypothesize a global feature of the defense/susceptibility mechanisms of citrus in response to the bacteria. These results have been explored in selection of target genes for genetic engineering to control HLB. Also, further transcriptome (RNAseq) experiments using tolerant and susceptible citrus genotypes infected with CaLam or CaLas using different time points are in progress to investigate the dynamics of expression of these genes during early stages of infection.", "language": "en", "license": { "name": "Creative Commons Attribution 4.0", "short_name": "CC BY 4.0", "text": "Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.\n\nNo additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.", "url": "https://creativecommons.org/licenses/by/4.0" }, "keywords": [], "section": "Abstracts of Presentations at the 3rd International Research Conference on Huanglongbing", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/90z7j2hm", "frozenauthors": [ { "first_name": "V.", "middle_name": "", "last_name": "Mafra", "name_suffix": "", "institution": "Centro de Citricultura Sylvio Moreira, Cordeirópolis, São Paulo", "department": "None" }, { "first_name": "P.", "middle_name": "K.", "last_name": "Martins", "name_suffix": "", "institution": "Centro de Citricultura Sylvio Moreira, Cordeirópolis, São Paulo", "department": "None" }, { "first_name": "C.", "middle_name": "S.", "last_name": "Franscisco", "name_suffix": "", "institution": "Centro de Citricultura Sylvio Moreira, Cordeirópolis, São Paulo", "department": "None" }, { "first_name": "M.", "middle_name": "", "last_name": "Ribeiro-Alves", "name_suffix": "", "institution": "Fundação Osvaldo Cruz, Rio de Janeiro", "department": "None" }, { "first_name": "J.", "middle_name": "", "last_name": "Freitas-Astúa", "name_suffix": "", "institution": "Centro de Citricultura Sylvio Moreira, Cordeirópolis, São Paulo;\nEMBRAPA Cassava and Fruits, Cruz das Almas, Bahia, Brazil", "department": "None" }, { "first_name": "M.", "middle_name": "A.", "last_name": "Machado", "name_suffix": "", "institution": "Centro de Citricultura Sylvio Moreira, Cordeirópolis, São Paulo", "department": "None" } ], "date_submitted": "2014-12-22T23:57:58Z", "date_accepted": "2014-12-22T23:57:58Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/iocv_journalcitruspathology/article/41369/galley/30968/download/" } ] }, { "pk": 41370, "title": "Candidatus Liberibacter americanus induces significant reprogramming of the transcriptome of the susceptible citrus genotype", "subtitle": null, "abstract": "In Brazil, \nHuanglongbing\n (HLB) is caused by \nCandidatus \nLiberibacter americanus (CaLam) and \nCa. \nL. asiaticus (CaLas). Both species are vectored by the Asian citrus psyllid and are restricted to the phloem of infected citrus, where they promote a severe imbalance in the translocation of nutrients and other important substances along the plant. Several studies of the transcriptional response of citrus to HLB have been reported, but only for infection caused by CaLas. This study evaluated the transcriptional reprogramming of a susceptible genotype (Pera sweet orange) challenged with CaLam, using a customized 385K microarray chip. The analyses showed that a large number of genes and biological processes were significantly altered upon CaLam infection. Among the changes we highlight induction of zinc transporters, modulation of enzymes related to sugar metabolism, decreased photosynthesis, induction of several defense-related genes and modulation of enzymes regulating ROS production. Several biological processes reported as differentially modulated upon infection with CaLas responded similarly to CaLam. The large number of receptor-like proteins, PR genes, NBS-LRR and transcription factors (such as WRKY and MYB) found showed that even a susceptible citrus genotype is able to actively respond to infection by CaLam, as reported for CaLas. Twenty candidate genes were selected for validation in symptomatic and asymptomatic PCR-positive leaves of Hamlin sweet orange infected with CaLas or CaLam. Finally, using \nin\n \nsilico\n approaches, we compared our results with all published studies using CaLas to hypothesize a global feature of the defense/susceptibility mechanisms of citrus in response to the bacteria. These results have been explored in selection of target genes for genetic engineering to control HLB. Also, further transcriptome (RNAseq) experiments using tolerant and susceptible citrus genotypes infected with CaLam or CaLas using different time points are in progress to investigate the dynamics of expression of these genes during early stages of infection.", "language": "en", "license": { "name": "Creative Commons Attribution 4.0", "short_name": "CC BY 4.0", "text": "Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.\n\nNo additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.", "url": "https://creativecommons.org/licenses/by/4.0" }, "keywords": [], "section": "Abstracts of Presentations at the 3rd International Research Conference on Huanglongbing", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/6hj4m66d", "frozenauthors": [ { "first_name": "V.", "middle_name": "", "last_name": "Mafra", "name_suffix": "", "institution": "Centro de Citricultura Sylvio Moreira, Cordeirópolis, São Paulo", "department": "None" }, { "first_name": "P.", "middle_name": "K.", "last_name": "Martins", "name_suffix": "", "institution": "Centro de Citricultura Sylvio Moreira, Cordeirópolis, São Paulo", "department": "None" }, { "first_name": "C.", "middle_name": "S.", "last_name": "Franscisco", "name_suffix": "", "institution": "Centro de Citricultura Sylvio Moreira, Cordeirópolis, São Paulo", "department": "None" }, { "first_name": "M.", "middle_name": "", "last_name": "Ribeiro-Alves", "name_suffix": "", "institution": "Fundação Osvaldo Cruz, Rio de Janeiro", "department": "None" }, { "first_name": "J.", "middle_name": "", "last_name": "Freitas-Astúa", "name_suffix": "", "institution": "Centro de Citricultura Sylvio Moreira, Cordeirópolis, São Paulo;\nEMBRAPA Cassava and Fruits, Cruz das Almas, Bahia, Brazil", "department": "None" }, { "first_name": "M.", "middle_name": "A.", "last_name": "Machado", "name_suffix": "", "institution": "Centro de Citricultura Sylvio Moreira, Cordeirópolis, São Paulo", "department": "None" } ], "date_submitted": "2014-12-22T23:58:12Z", "date_accepted": "2014-12-22T23:58:12Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/iocv_journalcitruspathology/article/41370/galley/30969/download/" } ] }, { "pk": 41301, "title": "Candidatus Liberibacter asiaticus causes damage to citrus fibrous roots before visual decline of Huanglongbing-infected citrus trees", "subtitle": null, "abstract": "Huanglongbing (HLB), associated with \nCandidatus\n Liberibacter asiaticus (\nLas\n), was first detected in Florida in late 2005 and is now widely distributed throughout the commercial citrus-growing regions. In recent seasons, concurrent with freeze and drought episodes, symptomatic HLB-infected trees were much more affected by the extremes of temperature and moisture than trees without HLB. Symptoms exhibited by the stressed trees were excessive leaf loss and premature fruit drop even when HLB-infected trees were managed with good nutritional and irrigation practices recommended to support health of HLB-affected trees. This stress intolerance may be due to a loss of fibrous roots. To assess root status of HLB-infected trees on Swingle citrumelo rootstock (\nCitrus paradisi\n × \nPoncirus trifoliata\n), blocks of 2,307 three-yr-old Hamlin orange trees and 2,693 four-yr-old Valencia orange trees were surveyed visually and with a real time polymerase chain reaction (PCR) assay to determine \nLas\n infection status. The incidence of \nLas\n-infected trees (pre-symptomatic: \nLas\n+, visually negative, and symptomatic: \nLas\n+, visually positive) trees was 89% for the Hamlin block and 88% for the Valencia block. \nLas\n+ trees had 30 and 37% lower fibrous root mass density for pre-symptomatic and symptomatic trees, respectively, compared to \nLas\n- trees. In a second survey, 10- to 25-yr-old Valencia trees on ‘Swingle’ citrumelo or ‘Carrizo’ citrange (\nC. sinensis\n (L.) × \nP. trifoliata\n) rootstock were sampled within 3-6 months after identification of visual HLB status as symptomatic (\nLas\n+, visually positive) or non-symptomatic (\nLas\n-, visually negative) in orchards located in the central ridge, south-central and southwest flatwoods. Pairs of HLB symptomatic and non-symptomatic trees were evaluated for PCR status, fibrous root mass density and \nPhytophthora nicotianae\n progagules in the rhizosphere soil. \nLas\n+ trees had 27-40% lower fibrous root mass density and in one location higher \nP. nicotianae\n per root but Phytophthora populations per cm3 soil were high on both \nLas\n+ and \nLas\n- trees. Fibrous root loss from HLB damage interacted with \nP. nicotianae\n depending on orchard location and time of year.", "language": "en", "license": { "name": "Creative Commons Attribution 4.0", "short_name": "CC BY 4.0", "text": "Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.\n\nNo additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.", "url": "https://creativecommons.org/licenses/by/4.0" }, "keywords": [], "section": "Abstracts of Presentations at the 3rd International Research Conference on Huanglongbing", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/5jn2v2hd", "frozenauthors": [ { "first_name": "J.", "middle_name": "H.", "last_name": "Graham", "name_suffix": "", "institution": "Univ. Florida, CREC, Lake Alfred, FL, USA", "department": "None" }, { "first_name": "E.", "middle_name": "G.", "last_name": "Johnson", "name_suffix": "", "institution": "Univ. Florida, CREC, Lake Alfred, FL, USA", "department": "None" }, { "first_name": "T.", "middle_name": "R.", "last_name": "Gottwald", "name_suffix": "", "institution": "USDA-ARS, Fort Pierce, FL, USA", "department": "None" }, { "first_name": "M.", "middle_name": "S.", "last_name": "Irey", "name_suffix": "", "institution": "US Sugar Corp., Clewiston FL, USA", "department": "None" } ], "date_submitted": "2014-12-17T19:26:42Z", "date_accepted": "2014-12-17T19:26:42Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/iocv_journalcitruspathology/article/41301/galley/30900/download/" } ] }, { "pk": 41354, "title": "‘Candidatus Liberibacter asiaticus’ Encodes Two Novel Autotransporters that Target to Mitochondria", "subtitle": null, "abstract": "As a phloem-limited, intracellular bacterial pathogen, ‘\nCandidatus\n Liberibacter asiaticus’\n \n(Las) has a significantly reduced genome and causes huanglongbing (HLB), a devastating disease of citrus worldwide. In this study, we characterized two novel autotransporter proteins of Las, and redesignated them as LasAI and LasAII in lieu of the previous names HyvI and HyvII. Proteins secreted by the type V secretion system (T5SS), known as autotransporters, are large extracellular virulence proteins localized to the bacterial poles. Bioinformatic analyses revealed that LasAI and LasAII share the structural features of an autotransporter family containing large repeats of a passenger domain and a unique C-terminal translocator domain. When fused to the GFP gene and expressed in \nE. coli\n, the LasAI C-terminus and the full length LasAII were localized to the bacterial poles, similar to other members of autotransporter family. Despite the absence of the signal peptide, LasAI was found to localize at the cell surface by immuno-dot blot using a monoclonal antibody against the partial LasAI protein. Its surface localization was also confirmed by the removal of the LasAI antigen using a proteinase K treatment of the intact bacterial cells. When co-inoculated with a P19 gene silencing suppressor and transiently expressed in tobacco leaves, the GFP-LasAI translocator targeted to the mitochondria. This is the first report that Las encodes novel autotransporters that target to mitochondria. These findings may lead to a better understanding of the pathogenesis of this intracellular “energy parasitic” bacterium, and to more efficient characterizing new molecular targets for HLB control.", "language": "en", "license": { "name": "Creative Commons Attribution 4.0", "short_name": "CC BY 4.0", "text": "Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.\n\nNo additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.", "url": "https://creativecommons.org/licenses/by/4.0" }, "keywords": [], "section": "Abstracts of Presentations at the 3rd International Research Conference on Huanglongbing", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/79c2350x", "frozenauthors": [ { "first_name": "Guixia", "middle_name": "", "last_name": "Hao", "name_suffix": "", "institution": "USDA-ARS-USHRL, Fort Pierce, Florida 34945", "department": "None" }, { "first_name": "Michael", "middle_name": "", "last_name": "Boyle", "name_suffix": "", "institution": "Smithsonian Marine Station, 701 Seaway Drive, Fort Pierce, FL 34949", "department": "None" }, { "first_name": "Lijuan", "middle_name": "", "last_name": "Zhou", "name_suffix": "", "institution": "USDA-ARS-USHRL, Fort Pierce, Florida 34945", "department": "None" }, { "first_name": "Yongping", "middle_name": "", "last_name": "Duan", "name_suffix": "", "institution": "USDA-ARS-USHRL, Fort Pierce, Florida 34945", "department": "None" } ], "date_submitted": "2014-12-20T02:38:34Z", "date_accepted": "2014-12-20T02:38:34Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/iocv_journalcitruspathology/article/41354/galley/30953/download/" } ] }, { "pk": 41332, "title": "Candidatus Liberibacter asiaticus titers in citrus cultivars in the field and in Asian Citrus Psyllid-inoculated greenhouse trees", "subtitle": null, "abstract": "A survey of seven citrus cultivars (\nCitrus\n sinensis, \nC.\n paradisi, ‘Temple’ tangor, ‘Minneola’ and ‘Orlando’ tangelos, and ‘Fallglo’ and ‘Sunburst’ mandarin hybrids) growing in commercial citrus orchards in Florida revealed a strong correlation between HLB incidence and severity and \nCandidatus\n Liberibacter asiaticus (CLas) titer (Stover and McCollum, 2011). Temple tangor and grapefruit consistently exhibited the least severe HLB symptoms and lowest CLas titers, followed in increasing order of HLB symptoms by ‘Fallglo’ and C. sinensis.\n \nIn the orchard, \nCandidatus\n Liberibacter asiaticus (CLas) is transmitted to citrus hosts via the Asian citrus psyllid (ACP); however, in most greenhouse studies CLas inoculation of citrus has been conducted by grafting. In an attempt to more closely mimic the natural process of CLas transmission by ACP to citrus we conducted a greenhouse study that included CLas-infected citron (\nCitrus medica\n) to serve as a source of inoculum, free-ranging ACP to vector the pathogen, and 16 citrus genotypes (\nC. medica\n,\n C. reticulata\n,\n C. grandis\n,\n C. sinensis\n,\n C. paradisi\n, \nPoncirus trifoliata\n, and nine citrus hybrids) as hosts. Our objectives were to determine the incidence of CLas infection and titer among the 16 genotypes over time. The experiment was conducted three times. Leaf samples were collected at regular intervals over a period of ca. 300 days, and each sample was assayed for the presence of CLas. In each experiment, CLas titer remained at less than 101 copies 16S rDNA g-1 fwt until 150 to 175 days after placing CLas negative trees into the greenhouse, and thereafter increased steadily for the remainder of the experiments. After 300 to 350 days in the greenhouse grand means for CLas titer ranged from 103 to 105 g-1 fwt, although HLB symptoms were not apparent. Significant differences in CLas titer among the cultivars were first detected at approximately 125 days and throughout the remainder of the experiment. Our results have significant implications for studies involving transmission of CLas by ACP and subsequent disease development.", "language": "en", "license": { "name": "Creative Commons Attribution 4.0", "short_name": "CC BY 4.0", "text": "Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.\n\nNo additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.", "url": "https://creativecommons.org/licenses/by/4.0" }, "keywords": [], "section": "Abstracts of Presentations at the 3rd International Research Conference on Huanglongbing", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/36s4n2t7", "frozenauthors": [ { "first_name": "Greg", "middle_name": "", "last_name": "McCollum", "name_suffix": "", "institution": "USDA-ARS, Fort Pierce, USA", "department": "None" }, { "first_name": "Mark", "middle_name": "", "last_name": "Hilf", "name_suffix": "", "institution": "USDA-ARS, Fort Pierce, USA", "department": "None" }, { "first_name": "Mike", "middle_name": "", "last_name": "Irey", "name_suffix": "", "institution": "US Sugar Corp., Clewiston, USA", "department": "None" } ], "date_submitted": "2014-12-18T19:53:16Z", "date_accepted": "2014-12-18T19:53:16Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/iocv_journalcitruspathology/article/41332/galley/30931/download/" } ] }, { "pk": 41238, "title": "Candidatus Liberibacter asisticus detection in the leaves, roots from infected trees and leaves of new shoots from the stumps of the infected sweet orange trees in Texas", "subtitle": null, "abstract": "A total of 108 root and corresponding symptomatic leaf samples from four different quadrants were collected from 27 6 year old sweet orange trees in which the presence of \nCandidatus \nLiberibacter asiaticus (CLas) was previously confirmed by quantitative polymerase chain reaction (qPCR). There was no significant difference (p>0.05) in the level of infection as determined by threshold cycle (Ct) values between different types of tissue tested. Analysis of variance (ANOVA) showed that there is no significant difference (p>0.05) in test results among different distances from the trunk or quandrants where the root samples were collected. The stumps of the infected trees were covered in a psyllid-proof cage and leaves from the new shoots emerged from these stumps did not show the presence of CLas where as the roots showed the presence of CLas. Moreover, there was no significant difference between roots from infected trees and roots from infected stumps. Additionally, there was a significant difference with an average Ct value difference of 2.97 cycles between the DNA samples extracted from roots using two different commercially available kits.", "language": "en", "license": { "name": "Creative Commons Attribution 4.0", "short_name": "CC BY 4.0", "text": "Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.\n\nNo additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.", "url": "https://creativecommons.org/licenses/by/4.0" }, "keywords": [], "section": "Abstracts of Presentations at the 3rd International Research Conference on Huanglongbing", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/4pr5g785", "frozenauthors": [ { "first_name": "Madhurababu", "middle_name": "", "last_name": "Kunta", "name_suffix": "", "institution": "Texas A&M University-Kingsville Citrus Center, 312 N. International Blvd, Weslaco, TX 78596, USA", "department": "None" }, { "first_name": "Carolina", "middle_name": "", "last_name": "de La Garza", "name_suffix": "", "institution": "Texas A&M University-Kingsville Citrus Center, 312 N. International Blvd, Weslaco, TX 78596, USA", "department": "None" }, { "first_name": "John", "middle_name": "V.", "last_name": "da Graça", "name_suffix": "", "institution": "Texas A&M University-Kingsville Citrus Center, 312 N. International Blvd, Weslaco, TX 78596, USA", "department": "None" }, { "first_name": "Mamoudou", "middle_name": "", "last_name": "Sétamou", "name_suffix": "", "institution": "Texas A&M University-Kingsville Citrus Center, 312 N. International Blvd, Weslaco, TX 78596, USA", "department": "None" }, { "first_name": "Eliezer", "middle_name": "S.", "last_name": "Louzada", "name_suffix": "", "institution": "Texas A&M University-Kingsville Citrus Center, 312 N. International Blvd, Weslaco, TX 78596, USA", "department": "None" } ], "date_submitted": "2014-11-20T00:07:52Z", "date_accepted": "2014-11-20T00:07:52Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/iocv_journalcitruspathology/article/41238/galley/30837/download/" } ] }, { "pk": 41360, "title": "‘Candidatus Liberibacter’ in four indigenous Rutaceous species from South Africa", "subtitle": null, "abstract": "‘\nCandidatus \nLiberibacter africanus’ (Laf), a phloem restricted, gram negative bacteria of the α-proteobacteria is the agent associated with Citrus greening disease in South Africa. A related bacterium \n‘Candidatus \nLiberibacter africanus spp. capensis’ (LafC) was previously described from an indigenous Rutaceae tree, \nCalodendrum capense. \n This led to the hypothesis that other indigenous Rutaceous trees may also be infected with Liberibacters related to either Laf or LafC. Samples from 289 \nVepris lanceolata, \n231 \nZanthoxylum capense, \nand 234 \nClausena anisata \nwere collected from within the natural distribution of these trees in South Africa. Total DNA was extracted and tested for the presence of a Liberibacter using a generic Liberibacter real-time PCR. ‘\nCandidatus \nLiberibacters’ present in positive samples were characterised by amplifying and sequencing the β-operon, 16S and \nomp \ngene regions. The percentage of Liberibacter positive samples differed per tree species with 6% \nV. lanceolata, \n4% \nZ. capense \nand 11% \nC. anisata \nrespectively, being infected. Phylogenetic analysis of the β-operon and \nomp \ngene regions, revealed unique phylogenetic clusters for Liberibacters associated with each tree species. Phylogenetic analysis from the 16S gene region however indicated that sequences obtained from \nV. lanceolata \nand \nC. anisata \nwere similar to 16S sequences for LafC, whereas that obtained from \nZ. capense \ngrouped on its own. Laf has not been identified from HLB-infected orchards from other Citrus producing countries other than Africa and the Mascarene Islands. The presence of related Liberibacters from indigenous Rutaceae species in South Africa may therefore suggest that Laf originated on the African continent.", "language": "en", "license": { "name": "Creative Commons Attribution 4.0", "short_name": "CC BY 4.0", "text": "Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.\n\nNo additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.", "url": "https://creativecommons.org/licenses/by/4.0" }, "keywords": [], "section": "Abstracts of Presentations at the 3rd International Research Conference on Huanglongbing", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/89j7441b", "frozenauthors": [ { "first_name": "R.", "middle_name": "", "last_name": "Viljoen", "name_suffix": "", "institution": "Department of Microbiology and Plant Pathology, University of Pretoria, Pretoria, RSA; \nForest and Agricultural Biotechnology Institute, University of Pretoria, Pretoria, RSA", "department": "None" }, { "first_name": "E.", "middle_name": "T.", "last_name": "Steenkamp", "name_suffix": "", "institution": "Department of Microbiology and Plant Pathology, University of Pretoria, Pretoria, RSA; \nForest and Agricultural Biotechnology Institute, University of Pretoria, Pretoria, RSA", "department": "None" }, { "first_name": "G.", "middle_name": "", "last_name": "Pietersen", "name_suffix": "", "institution": "Department of Microbiology and Plant Pathology, University of Pretoria, Pretoria, RSA;\nForest and Agricultural Biotechnology Institute, University of Pretoria, Pretoria, RSA;\nAgricultural Research Council - Plant Protection Research Institute, Pretoria, RSA", "department": "None" } ], "date_submitted": "2014-12-20T02:58:49Z", "date_accepted": "2014-12-20T02:58:49Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/iocv_journalcitruspathology/article/41360/galley/30959/download/" } ] }, { "pk": 37746, "title": "Carreño Bolívar, Rubí. Av. Independencia: Literatura, música e ideas de Chile disidente", "subtitle": null, "abstract": "Review of Av. Independencia", "language": "es", "license": { "name": "Copyright", "short_name": "Copyright", "text": "", "url": "https://escholarship.org/terms" }, "keywords": [], "section": "Reviews", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/327526fw", "frozenauthors": [ { "first_name": "Catalina", "middle_name": "", "last_name": "Forttes Zalaquett", "name_suffix": "", "institution": "Pontificia Universidad Católica de Valparaíso", "department": "None" } ], "date_submitted": "2015-04-28T03:11:44+01:00", "date_accepted": "2015-04-28T03:11:44+01:00", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/mester/article/37746/galley/28464/download/" } ] }, { "pk": 54892, "title": "Catullus and the Lyric Voice", "subtitle": null, "abstract": "The Lyric Voice can be explored to show the nexus of interlocutors clamouring to be heard in Catullus’ poetry, but ultimately, it is Catullus himself who frames and controls all interaction. In addressing his poems to specific people at specific times, Catullus attempts to be constantly present with the reader. He invites the reader to live the poem, to allow it to transcend the petty constraints of time and space, then elsewhere reminds the reader of the literary artifice which is innate in writing about writing. He points outside the poem, both to bring the readers into his world, but also to force them to recognise that it is fake and created. Playing with Sappho, Catullus recognises how liminal translation is, and questions the locus of the voice in that dialogue. Voice is most investigated through silence however, and Catullus explores everyone’s silences; those he addresses, the readers and even his own. Ultimately though, Catullus comes out on top, these are his poems, he is never silent, and to engage with him is to have your mouth filled with his words. Just as he silences those who can speak, he breathes life into a variety of personae loquentes that litter his poem, such as his phaselus, whose epigraphic tone helps Catullus capture and freeze a moment in time. The Lyric Voice exerts immense influence over how we interact with these poems, and if we listen closely, we can appreciate the voices Catullus does and does not allow us to hear.", "language": "en", "license": { "name": "", "short_name": "", "text": null, "url": "" }, "keywords": [ { "word": "Catullus" }, { "word": "Lyric Voice" }, { "word": "Poetic Perspective" }, { "word": "Sappho" }, { "word": "Metapoetry" }, { "word": "Epigraphs" }, { "word": "Literary Artifice" } ], "section": "Articles", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/5x98q959", "frozenauthors": [ { "first_name": "Qasim", "middle_name": "Zulfekar", "last_name": "Alli", "name_suffix": "", "institution": "", "department": "" } ], "date_submitted": "2014-02-07T20:19:04Z", "date_accepted": "2014-02-07T20:19:04Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/ucbclassics_bujc/article/54892/galley/41414/download/" } ] }, { "pk": 41378, "title": "Cell Penetrating Peptides as an Alternative Transformation Method in Citrus", "subtitle": null, "abstract": "Huanlongbing (HLB) has caused the loss of thousands of trees in Florida’s multi-billion dollar citrus industry. An effective, long-term strategy to controlling this disease will be by the incorporation of genetic resistance into commercial genotypes. Because conventional breeding is limited by the lack of natural resistance in citrus to HLB, genetic engineering is now considered a significant alternative to incorporating such characteristics. In fact, despite general concerns from the public against genetically modified organisms (GMOs), one National Academy report1 stated that genetic engineering will be the way to fully exterminate HLB, while growers’ support of a transgenic approach for disease resistant traits also continues to rise. The primary transformation method of citrus typically uses \nAgrobacterium\n, in which explants are suspended with the bacterium and subsequently placed on selection media. After treatment, the explants produce shoots that can ultimately lead to stable transgenic plants. Due to the slow growth and lengthy maturation, this\n \nprocess takes several years to produce reproductive trees and must be optimized for each cultivar. Consequently, transformation efficiency is substantially less than other model systems. The commercialization of transgenic disease resistant cultivars is even slower due to regulations limiting GMOs worldwide. In order to decrease the dependence upon bacterial vectors and increase transformation efficiency, we have researched an alternative method for introducing nucleic acids into plants that does not involve \nAgrobacterium \nand instead uses cell penetrating peptides (CPPs). CPPs are short, positively charged amino acid sequences that bind to negatively charged molecules and subsequently translocate across cellular membranes. Most surprisingly plant cell walls can also be bypassed, as CPPs are currently used in plants in transient expression and gene silencing assays. Until now, CPPs have not been examined in citrus or other woody crops for stable transformation protocols. We have developed a method for the transient expression of reporter genes (GUS and GFP) using plasmid DNA and CPPs. Our data indicate that up to 50% of treated explants express GUS when CPPs are used alone. Several optimization steps have been tested and the expression efficiency can be increased up to 100% when CPPs are used in conjunction with a lipid transfection reagent. We have also produced hypocotyl segments which survived kanamycin selection. Some produced shoots that rooted and were planted in soil and have been maintained in a growth chamber. PCR and reporter gene analysis will confirm if stable integration has occurred. Our novel protocol could have far reaching effects for the successful integration of disease resistant GMOs in global markets by limiting the perceived negative effect of bacterial vectors.", "language": "en", "license": { "name": "Creative Commons Attribution 4.0", "short_name": "CC BY 4.0", "text": "Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.\n\nNo additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.", "url": "https://creativecommons.org/licenses/by/4.0" }, "keywords": [], "section": "Abstracts of Presentations at the 3rd International Research Conference on Huanglongbing", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/8hs6r4zj", "frozenauthors": [ { "first_name": "Shaun", "middle_name": "P.", "last_name": "Jensen", "name_suffix": "", "institution": "", "department": "None" }, { "first_name": "Vicente", "middle_name": "J.", "last_name": "Febres", "name_suffix": "", "institution": "", "department": "None" }, { "first_name": "Gloria", "middle_name": "A.", "last_name": "Moore", "name_suffix": "", "institution": "", "department": "None" } ], "date_submitted": "2014-12-23T19:23:35Z", "date_accepted": "2014-12-23T19:23:35Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/iocv_journalcitruspathology/article/41378/galley/30977/download/" } ] }, { "pk": 41352, "title": "Characterization of putative virulent factors of Candidatus Liberibacter asiaticus", "subtitle": null, "abstract": "Citrus greening or huanglongbing (HLB) is a devastating disease of citrus, and poses a major threat to the citrus industry in the United States (1, 2). \nCandidatus\n Liberibacter asiaticus has been known to be associated with HLB in the United States (3, 4). Unsuccessful attempts to culture \nCa\n. L. asiaticus have notably hampered efforts to understand its biology and pathogenesis mechanism despite some limited progresses in culturing. In order to characterize the putative virulence factors, we expressed putative virulent factors in \nNicotiana benthamiana\n. Totally 24 putative virulent factors are being tested with most of them containing signal peptides. By transient expression of the candidates using TMV vector in \nN. benthamiana\n, we can screen the genes influencing plant development and morphology. Meanwhile, transformation of candidate genes into \nN. benthamiana\n driven by 35S promoter and phloem specific promoter respectively will further verify the function of putative virulence factors. Identification and characterization of the various virulence factors in \nCa\n. L. asiaticus will advance the understanding of the biology and pathogenicity of the pathogen.", "language": "en", "license": { "name": "Creative Commons Attribution 4.0", "short_name": "CC BY 4.0", "text": "Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.\n\nNo additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.", "url": "https://creativecommons.org/licenses/by/4.0" }, "keywords": [], "section": "Abstracts of Presentations at the 3rd International Research Conference on Huanglongbing", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/3d65p4ht", "frozenauthors": [ { "first_name": "Xiaobao", "middle_name": "", "last_name": "Ying", "name_suffix": "", "institution": "Citrus Research and Education Center, Department of Microbiology and Cell Science, University of Florida, 700 Experiment Station Road, Lake Alfred 33850, U.S.A.", "department": "None" }, { "first_name": "Nian", "middle_name": "", "last_name": "Wang", "name_suffix": "", "institution": "Citrus Research and Education Center, Department of Microbiology and Cell Science, University of Florida, 700 Experiment Station Road, Lake Alfred 33850, U.S.A.", "department": "None" } ], "date_submitted": "2014-12-20T02:31:15Z", "date_accepted": "2014-12-20T02:31:15Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/iocv_journalcitruspathology/article/41352/galley/30951/download/" } ] }, { "pk": 41339, "title": "Characterization of the microbial community structure in ‘Candidatus Liberibacter asiaticus’-infected citrus plants treated with antibiotics", "subtitle": null, "abstract": "The updated PhyloChip\nTM\n G3 were used to explore the differences in the relative abundance and phylogenetic diversity of the bacterial communities associated with HLB-affected citrus plants in the field over a growing season and those treated with antibiotic combinations of AG (Ampicillin at 1000 mg/L and Gentamicin at 100 mg/L) and PS (Penicillin at 1000 mg/L and Streptomycin at 100 mg/L). Both antibiotic treatments resulted in significantly lower Las bacterial titers (\nPr\n<0.05) and their hybridization scores. Of the 50,000+ available operational taxonomic units (OTUs) on PhyloChip™ G3, 7,028 known OTUs in 58 phyla were detected from the field plants, and 7,407 OTUs in 53 phyla from the inoculated potted plants. Proteobacteria\n \nwas the constantly dominant phylum of bacteria (38.7%~44.1%) vying for prevalence based on the season, followed by Firmicutes (23.5%~29.0%), Actinobacteria (12.4%~16.1%), Bacteroidetes (6.2%~6.6%) and Cyanobacteria (2.3%~3.2%). Circular tree comparing the Las-free and the Las-infected samples indicated that only 17 families present in the Las-free plants, such as \nCyanobacteriaceae\n; but more than 137 families detected in the Las-infected plants, such as \nStaphylococcaceae\n and \nPseudonocardiaceae.\n \nCyanobacteria\n are believed to be responsible for introducing oxygen into the atmosphere and fixing nitrogen and phosphorus assimilation for plant growth. Both \nStaphylococcaceae\n and \nPseudonocardiaceae\n were \nrecognized as an emerging opportunistic pathogen of plant and animals. When compared to the bacterial populations in the leaves of citrus trees receiving the water control treatment, the \nBacteroidete \npopulation decreased (\nPr\n<0.05) by 59.6% and 51.8% in the plants receiving AG and PS treatments, respectively. The overall diversity of bacteria also decreased with the antibiotic treatments. Bacterial cells in close proximity may be able to modify their microenvironment; thus, making the composition of the microbial community an important factor in the ability of Las to cause HLB progression. A low Las level was seen as a both a seasonal fluctuation, part of the bacterial population dynamics, and as a response to the antibiotic treatments.", "language": "en", "license": { "name": "Creative Commons Attribution 4.0", "short_name": "CC BY 4.0", "text": "Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.\n\nNo additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.", "url": "https://creativecommons.org/licenses/by/4.0" }, "keywords": [], "section": "Abstracts of Presentations at the 3rd International Research Conference on Huanglongbing", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/78n6q2rw", "frozenauthors": [ { "first_name": "M.", "middle_name": "", "last_name": "Zhang", "name_suffix": "", "institution": "Indian River Research and Education Center, IFAS-UF, Fort Pierce, FL34945, USA;\nUSDA-ARS, US Horticultural Lab, Fort Pierce, FL34945, USA;\nState Key Lab for Conservation and Utilization of Subtroical Agro-bioresources, Guangxi Univ., Nanning, Guangxi, CHINA 530004", "department": "None" }, { "first_name": "Charles", "middle_name": "A.", "last_name": "Powell", "name_suffix": "", "institution": "Indian River Research and Education Center, IFAS-UF, Fort Pierce, FL34945, USA", "department": "None" }, { "first_name": "Yu", "middle_name": "", "last_name": "Chuan", "name_suffix": "", "institution": "Indian River Research and Education Center, IFAS-UF, Fort Pierce, FL34945, USA", "department": "None" }, { "first_name": "Yongping", "middle_name": "", "last_name": "Duan", "name_suffix": "", "institution": "USDA-ARS, US Horticultural Lab, Fort Pierce, FL34945, USA", "department": "None" } ], "date_submitted": "2014-12-19T00:22:12Z", "date_accepted": "2014-12-19T00:22:12Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/iocv_journalcitruspathology/article/41339/galley/30938/download/" } ] }, { "pk": 41261, "title": "Characterization of the RNA Interference Response in the Asian Citrus Psyllid", "subtitle": null, "abstract": "The Asian citrus psyllid (ACP), \nDiaphorina citri\n, is a major pest of citrus since it is the only known vector of ‘\nCandidatus \nLiberibactor’ species, the bacterium associated with citrus greening disease. Since control of the psyllid is the only effective defense so far against citrus greening, and heavy reliance upon pesticides is not sustainable, an RNA interference (RNAi) strategy for ACP control was investigated. RNA interference is an innate immune response triggered by the cellular uptake of double stranded RNA (dsRNA) and studies were conducted to determine if ACP could be killed by oral uptake of dsRNA that target essential ACP transcripts. An artificial diet system was designed that facilitated the ingestion of dsRNA complementary to genes involved in digestion. Using this system, ACP mortality was observed as a result of oral uptake of dsRNAs targeting an apparent essential ACP gene and this mortality was shown to be dose responsive, reaching a maximum of 37% at high dsRNA concentrations. There was also what appeared to be sequence independent ACP toxicity of large doses of dsRNA, concentrations above 48 ng/uL in the diet. However, this sequence independent mortality was not as high as that observed for the targeted ACP gene, never rising above 17%. These results provide support for the concept that RNAi could be adapted for use as a control strategy for the ACP.", "language": "en", "license": { "name": "Creative Commons Attribution 4.0", "short_name": "CC BY 4.0", "text": "Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.\n\nNo additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.", "url": "https://creativecommons.org/licenses/by/4.0" }, "keywords": [], "section": "Abstracts of Presentations at the 3rd International Research Conference on Huanglongbing", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/3pz039x9", "frozenauthors": [ { "first_name": "Lindsay", "middle_name": "", "last_name": "Shaffer", "name_suffix": "", "institution": "", "department": "None" }, { "first_name": "R.", "middle_name": "G.", "last_name": "Shatters, Jr.", "name_suffix": "", "institution": "", "department": "None" }, { "first_name": "C.", "middle_name": "", "last_name": "Powell", "name_suffix": "", "institution": "", "department": "None" }, { "first_name": "R.", "middle_name": "", "last_name": "Cave", "name_suffix": "", "institution": "", "department": "None" }, { "first_name": "D.", "middle_name": "", "last_name": "Borovsky", "name_suffix": "", "institution": "", "department": "None" } ], "date_submitted": "2014-11-25T20:41:23Z", "date_accepted": "2014-11-25T20:41:23Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/iocv_journalcitruspathology/article/41261/galley/30860/download/" } ] }, { "pk": 37755, "title": "[Cher ami, Je suis en retard avec toi…]", "subtitle": null, "abstract": "Carta surrealista/poema visual dirigido al poeta peruano Emilio Adolfo Westphalen por César Moro, fechada en México, D.F. el 25 de setiembre de 1941", "language": "fr", "license": { "name": "Copyright", "short_name": "Copyright", "text": "", "url": "https://escholarship.org/terms" }, "keywords": [ { "word": "Cesar Moro" }, { "word": "Peruvian Poetry, Poesia Peruana, Surrealism, Surrealismo" } ], "section": "Translation", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/6v9348d1", "frozenauthors": [ { "first_name": "César", "middle_name": "", "last_name": "Moro", "name_suffix": "", "institution": "", "department": "None" } ], "date_submitted": "2015-12-07T00:29:23Z", "date_accepted": "2015-12-07T00:29:23Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/mester/article/37755/galley/28471/download/" } ] }, { "pk": 37758, "title": "“Cher ami, Je suis en retard avec toi…”: Una carta-poema surrealista de César Moro (1941)", "subtitle": null, "abstract": "Carta-poema surrealista de César Moro a Emilio Adolfo Westphalen escrita en 1941", "language": "es", "license": { "name": "Copyright", "short_name": "Copyright", "text": "", "url": "https://escholarship.org/terms" }, "keywords": [ { "word": "Cesar Moro" }, { "word": "Peruvian Poetry, Poesia Peruana, Surrealism, Surrealismo" } ], "section": "Translation", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/1sj05666", "frozenauthors": [ { "first_name": "Rafael", "middle_name": "", "last_name": "Ramírez Mendoza", "name_suffix": "", "institution": "UCLA", "department": "None" } ], "date_submitted": "2015-12-11T07:22:10Z", "date_accepted": "2015-12-11T07:22:10Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/mester/article/37758/galley/28473/download/" } ] }, { "pk": 52627, "title": "Chinatown: The Semi-Permeable Construction of Space and Time", "subtitle": null, "abstract": "", "language": "en", "license": { "name": "Creative Commons Attribution 4.0", "short_name": "CC BY 4.0", "text": "<p>Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use. No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.</p>", "url": "https://creativecommons.org/licenses/by/4.0" }, "keywords": [ { "word": "History" }, { "word": "Chinatown" }, { "word": "San Francisco" }, { "word": "Race" }, { "word": "Ethnicity" }, { "word": "Space" }, { "word": "newspapers" } ], "section": "Articles", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/5c0596fm", "frozenauthors": [ { "first_name": "Mario", "middle_name": "", "last_name": "Pulido", "name_suffix": "", "institution": "UC Merced", "department": "" } ], "date_submitted": "2014-05-15T19:39:55+01:00", "date_accepted": "2014-05-15T19:39:55+01:00", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/ssha_uhj/article/52627/galley/39683/download/" } ] }, { "pk": 6045, "title": "Ch'wichip in South Korean Female Identity: Untangling the Contradictions of Finding a Job and Getting Married", "subtitle": null, "abstract": "Ch'wichip in South Korean Female Identity: Untangling the Contradictions of Finding a Job and Getting Married", "language": "en", "license": { "name": "All rights reserved", "short_name": "Copyright", "text": "© the author(s). All rights reserved.", "url": "https://creativecommons.org/licenses/authors" }, "keywords": [], "section": "Articles", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/4bw5c3v9", "frozenauthors": [ { "first_name": "Yunhee", "middle_name": "", "last_name": "Roh", "name_suffix": "", "institution": "", "department": "None" } ], "date_submitted": "2014-11-23T05:34:15Z", "date_accepted": "2014-11-23T05:34:15Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "", "path": "https://journalpub.escholarship.org/our_buj/article/6045/galley/3683/download/" } ] }, { "pk": 41329, "title": "Citrus leaf volatiles response to ‘Candidatus Liberibacter asiaticus’ and to its insect vector Asian citrus psyllid", "subtitle": null, "abstract": "Plant volatiles play an important role in defending plants against insects and pathogens attack. Released volatiles from insect-damaged plants may result in direct or indirect defense against insect, and volatile accumulation in pathogen-infected plants may inhibit the movement of the pathogens within plant tissues. However, available information about the response of citrus leaf volatiles to Asian citrus psyllids (ACP) feeding and ‘\nCandidatus \nLiberibacter asiaticus’ (Clas) infection is limited. Here we investigate the effect of ACP feeding, Clas infection, and simultaneous attack by ACP and Clas on the volatile content of Valencia leaves. Leaf volatiles were extracted using hexane and analyzed with gas chromatography-mass spectrometry (GC-MS). Eighteen out of twenty-seven detected volatiles were induced (2 to 10-folds) in ACP-infested plants. On the other hand, only four volatiles were induced in Clas-infected plants (d-limonene, β-phelandrene, citronellal, and undecanl). The abundance of latter compounds was induced 4-folds in Clas-infected plants, except for limonene, which was induced more than 20-folds. In addition, citrus plants that were subjected to a simultaneous attack by ACP and Clas reduced their volatiles production compared to those that experience an attack only by ACP. Our results suggested that insect and pathogen attack not only changes the profile of plant released volatiles, but also influences the volatile contents of the attacked plants. The result of this study might contribute to better understanding of citrus response to ACP and Clas attacks and provide more information about the relation between stored and released volatiles.", "language": "en", "license": { "name": "Creative Commons Attribution 4.0", "short_name": "CC BY 4.0", "text": "Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.\n\nNo additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.", "url": "https://creativecommons.org/licenses/by/4.0" }, "keywords": [], "section": "Abstracts of Presentations at the 3rd International Research Conference on Huanglongbing", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/6kh9r7xb", "frozenauthors": [ { "first_name": "Faraj", "middle_name": "", "last_name": "Hijaz", "name_suffix": "", "institution": "", "department": "None" }, { "first_name": "Nabil", "middle_name": "", "last_name": "Killiny", "name_suffix": "", "institution": "University of Florida, Citrus Research and Education Center, FL, USA", "department": "None" } ], "date_submitted": "2014-12-18T19:40:12Z", "date_accepted": "2014-12-18T19:40:12Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/iocv_journalcitruspathology/article/41329/galley/30928/download/" } ] }, { "pk": 41300, "title": "Citrus tristeza virus-based RNA-interference (RNAi) vector and its potential in combating citrus Huanglongbing (HLB)", "subtitle": null, "abstract": "Citrus tristeza virus\n (CTV), a plus-sense ssRNA virus, is member of the genus Closterovirus, family \nClosteroviridae\n. RNA viruses are inducers as-well-as targets of gene silencing defense mechanism of host plants and this has been exploited as a tool in functional genomics. CTV was developed into virus-induced gene silencing (VIGS) or RNA-interference (RNAi) vector, which interferes with expression of endogenous genes in citrus or GFP-transgene in \nNicotiana benthamiana \n(16c) in a sequence specific manner. Photobleaching phenotype indicative of silencing of endogenous gene, phytoene desaturase in citrus, and red color under UV indicative of silencing of transgene GFP in \nN. benthamiana\n (16c) was observed using CTV-RNAi vector. CTV-RNAi vector has great potentials in combating huanglongbing (HLB) disease through (1) enhancing basal defense of citrus by silencing of auxin signaling F-Box receptor genes while simultaneously overexpressing microRNAs; (2) down-regulation of overexpressed genes, callose synthase and phloem protein-2, responsible for phloem-plugging in citrus by HLB; (3) expressing dsRNA specific to essential genes of insect vector psyllid (\nDiaphorina citri\n) to disable transmission of '\nCandidatus\n' Liberibacter asiaticus pathogen. Simultaneous silencing of multiple endogenous genes of a metabolic pathway is possible through tandem engineering of potential siRNA eliciting regions in CTV-RNAi vector.", "language": "en", "license": { "name": "Creative Commons Attribution 4.0", "short_name": "CC BY 4.0", "text": "Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.\n\nNo additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.", "url": "https://creativecommons.org/licenses/by/4.0" }, "keywords": [], "section": "Abstracts of Presentations at the 3rd International Research Conference on Huanglongbing", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/52d5x1fx", "frozenauthors": [ { "first_name": "Shubash", "middle_name": "", "last_name": "Hajeri", "name_suffix": "", "institution": "Citrus Research and Education Center, University of Florida, 700 Experiment Station Road,\nLake Alfred, FL 33850 USA", "department": "None" }, { "first_name": "Choaa", "middle_name": "", "last_name": "El-Mohtar", "name_suffix": "", "institution": "Citrus Research and Education Center, University of Florida, 700 Experiment Station Road,\nLake Alfred, FL 33850 USA", "department": "None" }, { "first_name": "William", "middle_name": "O.", "last_name": "Dawson", "name_suffix": "", "institution": "Citrus Research and Education Center, University of Florida, 700 Experiment Station Road,\nLake Alfred, FL 33850 USA", "department": "None" }, { "first_name": "Siddarame", "middle_name": "", "last_name": "Gowda", "name_suffix": "", "institution": "Citrus Research and Education Center, University of Florida, 700 Experiment Station Road,\nLake Alfred, FL 33850 USA", "department": "None" } ], "date_submitted": "2014-12-17T19:24:55Z", "date_accepted": "2014-12-17T19:24:55Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/iocv_journalcitruspathology/article/41300/galley/30899/download/" } ] }, { "pk": 41349, "title": "Clues into the metagenome of Huanglongbing infected Citrus by analysis of ancillary sequences from Ion Torrent whole genome Candidatus Liberibacter asiaticus sequencing", "subtitle": null, "abstract": "Huanglongbing (HLB) is a globally devastating disease of citrus. Presently, three etiological agents are associated with HLB and include; \nCandidatus\n Liberibacter asiaticus (CLas), \nCandidatus\n Liberibacter americanus; and \nCandidatus\n Liberibacter africanus. Attempts to determine alternate (non-Liberibacter) associated etiological agents of HLB have been performed, namely by metagenomic analyses with HLB phenotypic citrus of phloem tissue isolated from bark [1] and whole leaf midribs [2]. These reports indicated a strong correlation for Liberibacter species associated with HLB etiology, but they do not indicate the presence of other significant associated etiological agents. Utilizing both PCR and non-PCR based metagenomic strategies; these previous reports present an undersized view (relating to inherent technique limits, restricted sample scope, and/or bacterial bias) into the etiology of HLB. Here we report ancillary contiguous metagenomic sequences contained amongst whole CLas genome amplification contiguous sequences that were sequenced on the next generation Ion Torrent PGM sequencing system. Within these ancillary sequences, a diverse metagenomic community is present, indicating a rich diversity of both prokaryotic and eukaryotic organisms that are unique and in common within these globally isolated HLB citrus samples. Although this report does not represent a complete metagenomic study of HLB diseased citrus, it does suggest that the use of Ion Torrent PGM sequencing system can be employed for metagenomic analysis. These findings justify a more complete analysis of the metagenome of HLB etiology, which may help further elucidate the HLB disease complex.", "language": "en", "license": { "name": "Creative Commons Attribution 4.0", "short_name": "CC BY 4.0", "text": "Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.\n\nNo additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.", "url": "https://creativecommons.org/licenses/by/4.0" }, "keywords": [], "section": "Abstracts of Presentations at the 3rd International Research Conference on Huanglongbing", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/4b22q45m", "frozenauthors": [ { "first_name": "J.", "middle_name": "K.", "last_name": "Morgan", "name_suffix": "", "institution": "USDA-ARS USHRL, Fort Pierce, FL", "department": "None" }, { "first_name": "R.", "middle_name": "G.", "last_name": "Shatters", "name_suffix": "", "institution": "USDA-ARS USHRL, Fort Pierce, FL", "department": "None" }, { "first_name": "E.", "middle_name": "", "last_name": "Stover", "name_suffix": "", "institution": "USDA-ARS USHRL, Fort Pierce, FL", "department": "None" }, { "first_name": "Y.", "middle_name": "P.", "last_name": "Duan", "name_suffix": "", "institution": "USDA-ARS USHRL, Fort Pierce, FL", "department": "None" }, { "first_name": "G.", "middle_name": "A.", "last_name": "Moore", "name_suffix": "", "institution": "UF IFAS, Gainesville, FL", "department": "None" }, { "first_name": "C.", "middle_name": "A.", "last_name": "Powell", "name_suffix": "", "institution": "UF IRREC, Fort Pierce, FL", "department": "None" }, { "first_name": "A.", "middle_name": "", "last_name": "Jarra-Cavieres", "name_suffix": "", "institution": "UF IRREC, Fort Pierce, FL", "department": "None" }, { "first_name": "S.", "middle_name": "", "last_name": "Clark", "name_suffix": "", "institution": "UF IRREC, Fort Pierce, FL", "department": "None" } ], "date_submitted": "2014-12-20T01:47:51Z", "date_accepted": "2014-12-20T01:47:51Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/iocv_journalcitruspathology/article/41349/galley/30948/download/" } ] }, { "pk": 41335, "title": "Colonization and distribution patterns of Candidatus Liberibacter asiaticus in distinct citrus rootstocks", "subtitle": null, "abstract": "A study was conducted to assess (i) patterns of Las colonization in graft-inoculated 1-y-old seedlings of Cravo Rangpur lime, Sunki mandarin, and Swingle citrumelo and (ii) patterns of Las distribution in naturally infected 4 and 10-y-old trees of sweet oranges ‘Pera’ on Sunki and ‘Folha murcha’ on 11 rootstocks. The seedlings were inoculated at the trunk, 40 cm above the substrate level. Samples of bark were collected at the inoculation site, 10, 20, and 30 cm below it, and from the root 45, 75, 105, and 135 days after inoculation (dai). Samples from trees included symptomatic leaves and bark from the trunk (10 cm above or below the grafting line) and roots. The samples were analyzed through qPCR to estimate log Las genome/gram tissue. In seedlings Las colonization pattern was similar for all rootstocks. Las was detected in the root 45 dai or 3 months before the symptoms developed on leaves. In Swingle maxima titers were lower but reached a plateau faster (45 to 75 dai) than in Sunki or Cravo (75 to 135 dai) for all sampled sites. In the field the distribution pattern of Las in the trees was also similar for all rootstocks, with higher titers detected in the leaves (5.29±0.22) than in trunks (4.38±0.49; 3.78±0.27) or roots (3.38±0.28). No correlation existed between the amount of symptom on the canopy and Las titer in the root. This lack of correlation plus the fast movement of Las from the inoculation site down to the root may explain the failure of pruning to control HLB.", "language": "en", "license": { "name": "Creative Commons Attribution 4.0", "short_name": "CC BY 4.0", "text": "Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.\n\nNo additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.", "url": "https://creativecommons.org/licenses/by/4.0" }, "keywords": [], "section": "Abstracts of Presentations at the 3rd International Research Conference on Huanglongbing", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/6cg093tw", "frozenauthors": [ { "first_name": "S.", "middle_name": "A.", "last_name": "Lopes", "name_suffix": "", "institution": "Fundecitrus, Araraquara", "department": "None" }, { "first_name": "F.", "middle_name": "Q.B.F.", "last_name": "Luiz", "name_suffix": "", "institution": "EMBRAPA, Cruz das Alamas, Brazil", "department": "None" }, { "first_name": "E.", "middle_name": "S.", "last_name": "Stuchi", "name_suffix": "", "institution": "EMBRAPA, Cruz das Alamas, Brazil", "department": "None" } ], "date_submitted": "2014-12-19T00:07:16Z", "date_accepted": "2014-12-19T00:07:16Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/iocv_journalcitruspathology/article/41335/galley/30934/download/" } ] }, { "pk": 41343, "title": "Colonization of Seeds of Citrus Rootstock Varieties by ‘Candidatus Liberibacter asiaticus’", "subtitle": null, "abstract": "Huanglongbing (HLB) is a disease of citrus associated with a systemic infection by the α-proteobacterium ‘\nCandidatus\n Liberibacter asiaticus’. Infection of an individual tree can occur via psyllids (\nDiaphorina citri\n Kuwayama) carrying the bacterium or if the tree is propagated from infected budwood. Seed transmission is another possible mode of dissemination of the pathogen. Rootstock varieties are propagated from seed so we assessed the seed transmission among eighteen rootstock varieties using seeds from mature fruit collected in late winter and immature fruit collected in late summer. In dissected seeds real-time PCR detected pathogen DNA in seed coats at an incidence of 0-100%, whereas no pathogen DNA was detected in cotyledons or embryos from any variety. Seeds collected in late winter were germinated in a greenhouse and no pathogen DNA was detected in extracts of shoots of 425 seedlings harvested at 7-10 days post-germination whereas a small amount of pathogen DNA was detected in extracts from 6 of 425 roots. All six positive samples were the same rootstock variety, which had 100% colonization of seed coats. The positive samples likely are a result of remnant seed coat, which was not completely removed from cotyledons of the harvested seedlings. The data from this study suggest the pathogen can colonize the seed coat but it does not colonize embryos, which makes seed transmission unlikely.", "language": "en", "license": { "name": "Creative Commons Attribution 4.0", "short_name": "CC BY 4.0", "text": "Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.\n\nNo additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.", "url": "https://creativecommons.org/licenses/by/4.0" }, "keywords": [], "section": "Abstracts of Presentations at the 3rd International Research Conference on Huanglongbing", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/9488v15v", "frozenauthors": [ { "first_name": "Mark", "middle_name": "E.", "last_name": "Hilf", "name_suffix": "", "institution": "USDA-ARS, Fort Pierce, FL USA", "department": "None" } ], "date_submitted": "2014-12-19T00:34:04Z", "date_accepted": "2014-12-19T00:34:04Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/iocv_journalcitruspathology/article/41343/galley/30942/download/" } ] }, { "pk": 60719, "title": "Combined Heat and Power: A Technology Whose Time Has Come", "subtitle": null, "abstract": "On October 29, 2012, Hurricane Sandy blew through the largely populated areas of New Jersey, New York, and Connecticut. It was, at the time, the largest storm in the region’s history. While many areas lost electricity from the electrical grid, the few buildings equipped with Combined Heat and Power (“CHP”) remained lit and heated. For example, many residential and commercial facilities lost power for days after the storm, but natural gas powered CHP systems at the Co-Op City apartment complex and New York University, Fairfield University, and Princeton University kept their buildings functioning. According to Ross Tomlin, an employee of Gulf Coast Clean Energy Application Center of the Department of Energy, “because CHP relies on natural gas delivered through underground pipelines, [the systems] can weather just about any storm.” But minimizing the effects of natural disasters is only one of CHP’s many benefits. \nCHP is the process of capturing heat from existing heat sources, such as boilers, and using the heat to power energy sources, such as steam-powered turbines, to create electricity, hot water, and heat. The technology not only reduces energy costs through efficiency—at least twenty to thirty percent more efficient than separate heat and power systems—but it also protects the environment by burning less fuel, and thus reducing greenhouse gas emissions and air pollution. While this technology has seen continued barriers over the years, one company, Recycled Energy Development (“RED”), recognized that “the US lags far behind the world leaders when it comes to producing energy through [CHP]” and has taken steps to utilize the technology.\n \nThe average increase in energy costs for households between 2001 and 2012 was forty-three percent. This increase in energy costs affects consumers and businesses alike, with electricity costs topping many businesses’ lists of expenses. America undeniably faces a severe energy crisis both in the private sector, due to rising energy costs, and in the public sector, due to gridlock in government. Among the many green energy technologies currently available, CHP is “the least sexy” and considered “a ‘homeless’ suite of technologies” when compared to solar, wind, and hydropower energy systems. Recently, however, the federal government gave CHP a second look as it attempted to educate state governments and companies about the benefits of the CHP technology. The severe lack of effective and efficient state government incentive programs is hindering the CHP technology from reaching its full potential of providing cheap, sustainable power to businesses.\n \nThis Comment will argue that, given the policy benefits of the CHP technology, the federal government should create an organization to establish and monitor a CHP legislative blueprint with three financial incentive program options; states should establish two of those three financial incentive programs; and states should include CHP in their Renewable Portfolio Standards. This Comment will analyze the financial barriers hindering effective widespread use of CHP among private sector companies, examine current effective and ineffective state financial incentive programs, and determine which financial incentive regulations the federal government should include in the legislative blueprint. Because this is mainly a state law issue, no “one size fits all” approach will suffice; however, a legislative blueprint can educate state legislatures about the financial incentives that can be put in place to allow for effective and widespread use of the CHP technology. Such a legislative blueprint must include: 1) a rebate for installed costs of the technology, 2) a feed-in tariff to entice companies to re-funnel excess power through the grid system, and 3) a provision of grants to companies who successfully complete CHP installations.\n \nSection II includes a background discussion of the CHP technology that will help facilitate an understanding of how the technology works and what financial incentives previously existed. Section III includes an analysis of financial incentives, illustrated by state examples that will help analyze how the incentives operate. Section IV includes an analysis of states with little financial incentive programs and demonstrates why it hinders the CHP technology. Section V discusses the proposed solution to this problem, as introduced above.", "language": "en", "license": { "name": "", "short_name": "", "text": null, "url": "" }, "keywords": [ { "word": "Combined Heat and Power, CHP, co-generation, environment, environmental policy, environmental law" } ], "section": "Comments", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/74f9w21p", "frozenauthors": [ { "first_name": "Steven", "middle_name": "", "last_name": "Ferraina", "name_suffix": "", "institution": "", "department": "" } ], "date_submitted": "2014-01-04T16:30:41Z", "date_accepted": "2014-01-04T16:30:41Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/uclalaw_jelp/article/60719/galley/46683/download/" } ] }, { "pk": 41241, "title": "Commercial risk-based survey for HLB and implications for efficacy of Citrus Health Management Areas (CHMAs)", "subtitle": null, "abstract": "The USDA, APHIS, PPQ has been conducting a risk-based multi-pest survey (MPS) for the past two years. The initial 6 statewide survey cycles were conducted on a 6 week basis, whereas the subsequent 20 cycles have been conducted on a 3-week basis. HLB and ACP are the two main pests addressed by the MPS but other citrus pests and diseases, i.e., Asiatic Citrus Canker (ACC), Citrus Leprosis Virus (CLiV), Citrus Black Spot (CBS) and Citrus Variegated Chlorosis (CVC) were also given lesser emphasis during the survey.", "language": "en", "license": { "name": "Creative Commons Attribution 4.0", "short_name": "CC BY 4.0", "text": "Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.\n\nNo additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.", "url": "https://creativecommons.org/licenses/by/4.0" }, "keywords": [], "section": "Abstracts of Presentations at the 3rd International Research Conference on Huanglongbing", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/28w7r84b", "frozenauthors": [ { "first_name": "T.", "middle_name": "", "last_name": "Gottwald", "name_suffix": "", "institution": "USDA, ARS, US Horticultural Research Laboratory, Fort Pierce, Florida, USA", "department": "None" }, { "first_name": "W.", "middle_name": "", "last_name": "Luo", "name_suffix": "", "institution": "USDA, ARS, US Horticultural Research Laboratory, Fort Pierce, Florida, USA;\nCIPM, NC State University, Raleigh, North Carolina, USA", "department": "None" }, { "first_name": "T.", "middle_name": "", "last_name": "Riley", "name_suffix": "", "institution": "USDA, APHIS PPQ, Orlando, Florida, USA", "department": "None" }, { "first_name": "S.", "middle_name": "", "last_name": "Parnell", "name_suffix": "", "institution": "Rothamstead Research, Harpenden, UK", "department": "None" } ], "date_submitted": "2014-11-25T18:34:16Z", "date_accepted": "2014-11-25T18:34:16Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/iocv_journalcitruspathology/article/41241/galley/30840/download/" } ] }, { "pk": 56469, "title": "Communism and the Tutelage of African Agency: Revisiting Mandela’s Communist Ties", "subtitle": null, "abstract": "African liberation movements and Communist parties often collaborated in their efforts to remove the yoke of colonialism and imperialism from the African continent. This cooperation is not evidence of Communist parties dictating the affairs and decisions of these liberation movements. This inference may be applied to the African National Congress (ANC), Nelson Mandela, and the South African Communist Party (SACP). While the ANC and Mandela worked with Communists and the SACP, Mandela himself was not a Communist, nor did the SACP manipulate or dictate his actions. Mandela worked with Communists, and Communists joined the ANC, where they assumed high positions within that party. Both the ANC and SACP came to realize that their goals of removing colonialism and imperialism were identical and that they thus needed to work together. This essay looks at the relationship and symbioses between Mandela and the SACP, as well as popular media’s perceptions of this relationship.", "language": "en", "license": null, "keywords": [], "section": "Essays / Articles Part I: Re-understanding Biko, Mandela and Memory", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/43d050xv", "frozenauthors": [ { "first_name": "Nana", "middle_name": "", "last_name": "Osei-Opare", "name_suffix": "", "institution": "UCLA", "department": "" } ], "date_submitted": "2014-12-14T03:47:47Z", "date_accepted": "2014-12-14T03:47:47Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/ufahamu/article/56469/galley/42877/download/" } ] }, { "pk": 41347, "title": "Comparative genomics analysis of Liberibacter species to elucidate pathogenesis and culturability", "subtitle": null, "abstract": "Liberibacter crescens\n BT-1, a gram negative, rod-shaped bacterial isolate, was previously recovered from mountain papaya and sequenced. As L. crescens is culturable and is not a pathogen of citrus, comparative genomics of this strain with other uncultured, pathogenic \nLiberibacter\n should suggest genes involved in both phenotypes. Genomic comparison to \nLiberibacter \nsp\n.\n revealed differences in metabolic pathways and virulence genes. Specific differences in metabolism include the inability of \nL. asiaticus\n and \nL. solanacearum\n to synthesize histidine, tryptophan, and thiamine, as well as a reduced ability to produce other amino acids. \nL. asiaticus\n and \nL. solanacearum\n possess a thiamine ABC transporter not found in \nL. crescens\n, which may compensate for the inability to synthesize thiamine. Diversity in the flp pilus operon and metal ABC transporters were observed that may contribute to the pathogenesis of the uncultured species. These differences may contribute to variation in virulence and culturability among species. Finally, two putative prophage regions were found in \nL. crescens\n that share moderate functional similarity to the phage regions of \nL. asiaticus\n, but sequence similarity is not conserved. Additional genomic comparisons will expand our understanding of virulence and vector-interactions of the described\n Liberibacter \nsp.", "language": "en", "license": { "name": "Creative Commons Attribution 4.0", "short_name": "CC BY 4.0", "text": "Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.\n\nNo additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.", "url": "https://creativecommons.org/licenses/by/4.0" }, "keywords": [], "section": "Abstracts of Presentations at the 3rd International Research Conference on Huanglongbing", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/079419w7", "frozenauthors": [ { "first_name": "Michael", "middle_name": "T.", "last_name": "Leonard", "name_suffix": "", "institution": "Microbiology and Cell Science Department", "department": "None" }, { "first_name": "Jennie", "middle_name": "R.", "last_name": "Fagen", "name_suffix": "", "institution": "Microbiology and Cell Science Department", "department": "None" }, { "first_name": "Connor", "middle_name": "M.", "last_name": "McCullough", "name_suffix": "", "institution": "Microbiology and Cell Science Department", "department": "None" }, { "first_name": "Austin", "middle_name": "G.", "last_name": "Davis-Richardson", "name_suffix": "", "institution": "Microbiology and Cell Science Department", "department": "None" }, { "first_name": "Michael", "middle_name": "J.", "last_name": "Davis", "name_suffix": "", "institution": "Citrus Research and Education Center;\nPlant Pathology Department, Institute of Food and Agricultural Sciences, University of Florida", "department": "None" }, { "first_name": "Eric", "middle_name": "W.", "last_name": "Triplett", "name_suffix": "", "institution": "Microbiology and Cell Science Department", "department": "None" } ], "date_submitted": "2014-12-20T01:39:00Z", "date_accepted": "2014-12-20T01:39:00Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/iocv_journalcitruspathology/article/41347/galley/30946/download/" } ] }, { "pk": 41328, "title": "Comparison of micro RNA (miRNA) Profiles and Some miRNA Target Gene Expression levels in Roots of Non-infected and Huanglongbing-infected Tangerine (Citrus reticulata Blanco cv.‘Sanhu’ ) Trees", "subtitle": null, "abstract": "Solexa sequencing was used to reveal the changes in small RNAome profile in roots of mock-inoculated (CK) and Huanglongbing bacteria-inoculated (HLB) ‘Sanhu’ tangerine (\nCitrus reticulata \nBlanco) trees. Results showed that the number of reads of both unique and total sRNAs decreased apparently in roots following infection with HLB. Distribution in length of sRNAs changed also remarkably, showing an increase in 22 nt and 21 nt small RNAs (sRNAs) and a decrease in 24 nt sRNAs in HLB-infected samples. A total of 42 known micro RNAs (miRNAs) belonging to 27 highly conserved miRNA families were identified. Comparisons showed that 33 known miRNAs exhibited a significant expression difference between CK and HLB-inoculated samples. In addition, 34 novel miRNAs, among which 24 were differentially expressed, were also identified, and their expression levels were analyzed by qRT-PCR. Three hundred and eighty five potential target genes were predicted for most of the 57 differentially expressed miRNAs. GO and KEGG annotation analysis revealed that most miRNA-target genes were those implicated in developmental process, response to stress and stimulus, transcription and protein metabolism. The characterization of the miRNAomes between the healthy and HLB infected Sanhu tangerine roots provided new insight into the involvement of miRNAs in HLB infection of citrus.", "language": "en", "license": { "name": "Creative Commons Attribution 4.0", "short_name": "CC BY 4.0", "text": "Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.\n\nNo additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.", "url": "https://creativecommons.org/licenses/by/4.0" }, "keywords": [], "section": "Abstracts of Presentations at the 3rd International Research Conference on Huanglongbing", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/9k8846mf", "frozenauthors": [ { "first_name": "Yun", "middle_name": "", "last_name": "Zhong", "name_suffix": "", "institution": "Institution of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, China", "department": "None" }, { "first_name": "Chunzhen", "middle_name": "", "last_name": "Cheng", "name_suffix": "", "institution": "Institution of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, China; \nCitrus Research Institute, Southwest University, China", "department": "None" }, { "first_name": "Bo", "middle_name": "", "last_name": "Wu", "name_suffix": "", "institution": "Institution of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, China; \nCitrus Research Institute, Southwest University, China", "department": "None" }, { "first_name": "Xuejun", "middle_name": "", "last_name": "Bei", "name_suffix": "", "institution": "Institution of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, China;\nCitrus Research Institute, Southwest University, China", "department": "None" }, { "first_name": "Bo", "middle_name": "", "last_name": "Jiang", "name_suffix": "", "institution": "Institution of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, China", "department": "None" }, { "first_name": "Jiwu", "middle_name": "", "last_name": "Zeng", "name_suffix": "", "institution": "Institution of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, China", "department": "None" }, { "first_name": "Guangyan", "middle_name": "", "last_name": "Zhong", "name_suffix": "", "institution": "Institution of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, China;\nCitrus Research Institute, Southwest University, China", "department": "None" } ], "date_submitted": "2014-12-18T19:37:57Z", "date_accepted": "2014-12-18T19:37:57Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/iocv_journalcitruspathology/article/41328/galley/30927/download/" } ] }, { "pk": 41231, "title": "Comparison of optical sensing techniques for detecting citrus diseases", "subtitle": null, "abstract": "Citrus diseases such as Huanglongbing (HLB) and citrus canker result in major production and economic losses in Florida. Therefore, there is an urgent need for a sensing technique that can be used to detect these diseases and apply suitable management practices. An ideal sensing technique should be rapid, accurate and reliable to provide economic, production and agricultural benefits. Optical sensing techniques such as visible-near infrared spectroscopy offer rapid sensing of plant diseases. Although optical sensing methods have few limitations, they offer unique benefits that can greatly aid in citrus disease detection. We have worked on several spectroscopic and imaging techniques to detect citrus diseases, especially HLB. This work presents the comparative performance of these techniques and discusses the benefits and limitations of each of these methods. The spectral techniques that will be discussed in this paper are: visible-near infrared spectroscopy, mid-infrared spectroscopy, fluorescence spectroscopy, multiband imaging, and thermal imaging. We collected spectral data representing healthy and diseased leaves from the citrus trees of different cultivar, followed by pre- and post-processing offsite using mathematical models. The classification studies using Naïve-Bayes classifier, Bagged Decision trees and Support Vector Machine were performed before or after principal component analysis, depending on the dataset. Our results indicated that most of these techniques showed a classification accuracy of about 90% and higher. However, the suitability of a technique would depend on its application. This paper summarizes the major findings from different spectroscopic techniques and compares their performance in relation to their applications.", "language": "en", "license": { "name": "Creative Commons Attribution 4.0", "short_name": "CC BY 4.0", "text": "Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.\n\nNo additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.", "url": "https://creativecommons.org/licenses/by/4.0" }, "keywords": [], "section": "Abstracts of Presentations at the 3rd International Research Conference on Huanglongbing", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/6fq9p7wp", "frozenauthors": [ { "first_name": "Sindhuja", "middle_name": "", "last_name": "Sankaran", "name_suffix": "", "institution": "Citrus Research and Education Center, Institute of Food and Agricultural Science, University of Florida, 700 Experiment Station Road, Lake Alfred, FL 33880", "department": "None" }, { "first_name": "Reza", "middle_name": "", "last_name": "Ehsani", "name_suffix": "", "institution": "Citrus Research and Education Center, Institute of Food and Agricultural Science, University of Florida, 700 Experiment Station Road, Lake Alfred, FL 33880", "department": "None" } ], "date_submitted": "2014-11-19T23:47:14Z", "date_accepted": "2014-11-19T23:47:14Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/iocv_journalcitruspathology/article/41231/galley/30830/download/" } ] }, { "pk": 41269, "title": "Composition of citrus phloem sap and honeydew produced by the citrus phloem sap feeder, the Asian citrus psyllid, Diaphorina citri (Homopetera: Psyllidae)", "subtitle": null, "abstract": "The honeydew composition of Asian citrus psyllids (ACP), the vector of citrus Huanglongbing (HLB), was studied using gas chromatography-mass spectrometry (GC-MS). Honeydew samples were collected from healthy ACP that were reared on one-year Valencia trees inside an insectary at a University of Florida’s Citrus Research and Education Center (CREC) grove in Lake Alfred, FL, USA. Dried samples (1 mg) were mixed with 30 μL of methoxyamine hydrochloride solution in pyridine (2%) and allowed to react for 17 h at room temperature. After methoximation, silylation reactions were induced by adding 80 μL of N-methyl-N-trimethylsilyl) trifluoracetamide (MSTFA) for 2 h at room temperature and 0.5 μL of derivatized sample was injected into the GC-MS running in the full scan mode. To check for amino acids, a 10 mg of alkaline honeydew sample was reacted with methylchloroformate (MCF) in a mixture of pyridine and methanol. The MCF derivatives were extracted with chloroform and analyzed with GC-MS. The moisure content was determined by drying the honeydew samples to a constant weight at a 100 °C. The major honeydew composition was as follows: 74.5 ± 2.8 sucrose, 12.4 ± 0.5 D-fructose, 6.4 ± 3.0 mannose, 1.8 ± 0.6 trehalose, myo-inositol 2.8 ± 0.8, ribitol 0.5 ± 1, galactose 0.4 ± 0.2, quinic acid 0.4± 0.3, and malic acid 0.3 ± 0.1. The moisture content of honeydew was 22.6 ± 2.6 No amino acids were detected as TMS or as MCF derivatives.", "language": "en", "license": { "name": "Creative Commons Attribution 4.0", "short_name": "CC BY 4.0", "text": "Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.\n\nNo additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.", "url": "https://creativecommons.org/licenses/by/4.0" }, "keywords": [], "section": "Abstracts of Presentations at the 3rd International Research Conference on Huanglongbing", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/3h66p1tv", "frozenauthors": [ { "first_name": "Faraj", "middle_name": "", "last_name": "Hijaz", "name_suffix": "", "institution": "", "department": "None" }, { "first_name": "Nabil", "middle_name": "", "last_name": "Killiny", "name_suffix": "", "institution": "", "department": "None" } ], "date_submitted": "2014-11-25T20:56:20Z", "date_accepted": "2014-11-25T20:56:20Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/iocv_journalcitruspathology/article/41269/galley/30868/download/" } ] }, { "pk": 45178, "title": "Contemporary Remediations of Race and Ethnicity in German Visual Cultures", "subtitle": null, "abstract": "Introduction to TRANSIT, Volume 9, Issue 2, Special Topic: “Contemporary Remediations of Race and Ethnicity in German Visual Cultures,” guest edited by Angelica Fenner and Uli Linke.", "language": "en", "license": null, "keywords": [ { "word": "remediation, remediations, race, ethnicity, visual culture, new media, digital age, global memory culture, anthropology, globalization, special topic, representations" } ], "section": "Articles", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/012264q5", "frozenauthors": [ { "first_name": "Angelica", "middle_name": "", "last_name": "Fenner", "name_suffix": "", "institution": "University of Toronto", "department": "None" }, { "first_name": "Uli", "middle_name": "", "last_name": "Linke", "name_suffix": "", "institution": "Rochester Institute of Technology", "department": "None" } ], "date_submitted": "2014-12-17T13:38:26Z", "date_accepted": "2014-12-17T13:38:26Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/transit/article/45178/galley/33968/download/" } ] }, { "pk": 61111, "title": "Contents", "subtitle": null, "abstract": "", "language": "en", "license": { "name": "", "short_name": "", "text": null, "url": "" }, "keywords": [], "section": "Front Matter", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/9qh6f77k", "frozenauthors": [ { "first_name": "[No author]", "middle_name": "", "last_name": "PBLJ", "name_suffix": "", "institution": "", "department": "" } ], "date_submitted": "2014-03-30T18:52:32+01:00", "date_accepted": "2014-03-30T18:52:32+01:00", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/uclalaw_pblj/article/61111/galley/47080/download/" } ] }, { "pk": 56466, "title": "Contributors", "subtitle": null, "abstract": "[no abstract]", "language": "en", "license": null, "keywords": [], "section": "Contributors", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/76k89363", "frozenauthors": [ { "first_name": "Editorial Board", "middle_name": "", "last_name": "Ufahamu: A Journal of African Studies", "name_suffix": "", "institution": "UCLA", "department": "" } ], "date_submitted": "2014-12-14T03:38:37Z", "date_accepted": "2014-12-14T03:38:37Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/ufahamu/article/56466/galley/42874/download/" } ] }, { "pk": 59108, "title": "Copper Catalyzed Oceanic Methyl Halide Production", "subtitle": null, "abstract": "Methyl halides are found in all of Earth’s biomes, produced naturally or through manmade means. Their presence in the atmosphere is problematic, as they catalyze depletion of stratospheric ozone. To understand the full environmental impact of these compounds, it is important to identify their chemical cycling processes. Iron increases methyl halide production in soils and oceans, yet copper’s influence remains unknown despite its similar chemical oxidation properties to iron. I experimentally tested the effect of copper sulfate and sunlight on methyl halide fluxes in San Francisco Bay seawater. Samples exposed to copper sulfate and sunlight averaged higher positive flux rates than other treatments. Copper sulfate also increased carbon dioxide production and acidification of the water. The interaction of copper sulfate and sunlight in seawater suggests a new mechanism for methyl halide production, most likely via a photochemical reaction or through suppression of normal uptake processes causing overabundant concentrations.", "language": "en", "license": null, "keywords": [ { "word": "San Francisco Bay, ocean-atmosphere exchange, photochemical reaction, oxidation reaction, metals" } ], "section": "Research", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/3fd989mn", "frozenauthors": [ { "first_name": "Jae Yun", "middle_name": "Robin", "last_name": "Kim", "name_suffix": "", "institution": "University of California, Berkeley", "department": "" }, { "first_name": "Robert", "middle_name": "", "last_name": "Rhew", "name_suffix": "", "institution": "University of California, Berkeley", "department": "" } ], "date_submitted": "2014-04-29T02:50:58+01:00", "date_accepted": "2014-04-29T02:50:58+01:00", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/our_bsj/article/59108/galley/45136/download/" } ] }, { "pk": 61109, "title": "Copyright Law in the Republic of Korea", "subtitle": null, "abstract": "", "language": "en", "license": { "name": "", "short_name": "", "text": null, "url": "" }, "keywords": [], "section": "Articles", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/8b9199zm", "frozenauthors": [ { "first_name": "Kyu", "middle_name": "Ho", "last_name": "Youm", "name_suffix": "", "institution": "", "department": "" } ], "date_submitted": "2014-03-30T18:48:39+01:00", "date_accepted": "2014-03-30T18:48:39+01:00", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/uclalaw_pblj/article/61109/galley/47078/download/" } ] }, { "pk": 37747, "title": "Cornejo Polar, Antonio. Writing in the Air: Heterogeneity and the Persistence of Oral Tradition in Andean Literatures", "subtitle": null, "abstract": "Review of Writing in the Air by Antonio Cornejo Polar", "language": "en", "license": { "name": "Copyright", "short_name": "Copyright", "text": "", "url": "https://escholarship.org/terms" }, "keywords": [], "section": "Reviews", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/2kk7c414", "frozenauthors": [ { "first_name": "Stephen", "middle_name": "", "last_name": "Hart", "name_suffix": "", "institution": "University College London", "department": "None" } ], "date_submitted": "2015-04-28T05:31:52+01:00", "date_accepted": "2015-04-28T05:31:52+01:00", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/mester/article/37747/galley/28465/download/" } ] }, { "pk": 61236, "title": "Corruption in the Procurement of Pharmaceuticals and Medical Equipment in China: The Incentives Facing Multinationals, Domestic Firms and Hospital Officials", "subtitle": null, "abstract": "Calls for reform of the Chinese healthcare system are voiced at the highest levels of the Chinese government, but reform cannot succeed unless policymakers confront the incentives for corruption built into the institutional structure of the healthcare system. Focusing on the markets for pharmaceuticals and medical equipment, this article isolates the special features of the Chinese healthcare system that are conducive to corruption. Without denying the responsibility of individual corporate representatives (both domestic and foreign), middlemen, and healthcare professionals, this article looks beyond the individual deals to document the underlying incentives for corruption by hospitals, physicians, and companies and explains how corrupt practices are currently organized. Finally, we argue that curbing the prevalent corruption requires efforts from both private companies and from the government.", "language": "en", "license": { "name": "", "short_name": "", "text": null, "url": "" }, "keywords": [ { "word": "China" }, { "word": "healthcare" }, { "word": "hospitals" }, { "word": "pharmaceuticals" }, { "word": "medical equipment" }, { "word": "multinationals" }, { "word": "corruption" }, { "word": "kickbacks" } ], "section": "Articles", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/5742d68k", "frozenauthors": [ { "first_name": "Susan", "middle_name": "", "last_name": "Rose-Ackerman", "name_suffix": "", "institution": "", "department": "" }, { "first_name": "Yingqi", "middle_name": "", "last_name": "Tan", "name_suffix": "", "institution": "", "department": "" } ], "date_submitted": "2015-07-06T18:29:11+01:00", "date_accepted": "2015-07-06T18:29:11+01:00", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/uclalaw_pblj/article/61236/galley/47275/download/" } ] }, { "pk": 61233, "title": "Corruption or \nGuanxi\n? Differentiating Between the Legitimate, Unethical, and Corrupt Activities of Chinese Government Officials", "subtitle": null, "abstract": "China has a well-documented corruption problem that has continued for decades, evolving concurrently with China’s economy and various institutional structures. In analyzing China’s corruption problem, the current definitions of corruption are inadequate to account for China’s guanxi culture, which requires gift giving in order to facilitate relationship building. By some definitions, the behaviors that \nguanxi\n culture mandates for Chinese society are corrupt when government officials engage in them, perhaps even implying that Chinese culture itself is corrupt. This is a mistake because it distracts from the actual causes of corruption. China’s corruption problem is caused by institutional and structural flaws that provide opportunities and incentives for corruption that would be exploited regardless of \nguanxi\n culture. Thus, it is important to explicitly exclude legitimate \nguanxi\n practices from the definition of corruption in order to bring into better focus the institutional, structural and procedural flaws that cause corruption and to provide workable solutions.", "language": "en", "license": { "name": "", "short_name": "", "text": null, "url": "" }, "keywords": [], "section": "Articles", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/0p8650mm", "frozenauthors": [ { "first_name": "Jacob", "middle_name": "", "last_name": "Harding", "name_suffix": "", "institution": "", "department": "" } ], "date_submitted": "2014-12-18T22:03:42Z", "date_accepted": "2014-12-18T22:03:42Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/uclalaw_pblj/article/61233/galley/47272/download/" } ] }, { "pk": 20976, "title": "Costa Del Sol: Living Between the Lines", "subtitle": null, "abstract": "Our research has culminated in an experimental documentary that takes a close look at Costa del Sol and its voices. The film denies the linearity of the N-340 and depicts it as a cloud of vaguely located points, perceived and experienced by the area’s inhabitants. The film is divided into twelve episodes, apparently unrelated, that depict a divergent social landscape whose coherence is achieved through the collective sense of belonging. This urban imaginary brings to light a pronounced and invigorating diversity, a Costa del Sol that constructs itself according to the ways in which citizens relate with their environment.\n \nThe film can be seen in its entirety at: http://vimeo.com/84060889.", "language": "en", "license": { "name": "none", "short_name": "none", "text": "", "url": "https://escholarship.org/terms" }, "keywords": [], "section": "Article", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/69z3c544", "frozenauthors": [ { "first_name": "Chema", "middle_name": "", "last_name": "Segovia Collado", "name_suffix": "", "institution": "La Ciudad Construida (Valencia, Spain)", "department": "None" }, { "first_name": "Jesus", "middle_name": "", "last_name": "Quintanilla Azzarelli", "name_suffix": "", "institution": "La Ciudad Construida (Valencia, Spain)", "department": "None" } ], "date_submitted": "2014-11-24T00:10:47Z", "date_accepted": "2014-11-24T00:10:47Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/criticalplanning/article/20976/galley/10678/download/" } ] }, { "pk": 54887, "title": "Cover", "subtitle": null, "abstract": "", "language": "en", "license": { "name": "", "short_name": "", "text": null, "url": "" }, "keywords": [], "section": "Forematter", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/6dj2d79x", "frozenauthors": [ { "first_name": "BUJC", "middle_name": "", "last_name": "Editors", "name_suffix": "", "institution": "", "department": "" } ], "date_submitted": "2014-02-06T23:17:40Z", "date_accepted": "2014-02-06T23:17:40Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/ucbclassics_bujc/article/54887/galley/41409/download/" } ] }, { "pk": 54911, "title": "Cover", "subtitle": null, "abstract": "", "language": "en", "license": { "name": "", "short_name": "", "text": null, "url": "" }, "keywords": [], "section": "Forematter", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/1qc0j9g6", "frozenauthors": [ { "first_name": "BUJC", "middle_name": "", "last_name": "Editors", "name_suffix": "", "institution": "", "department": "" } ], "date_submitted": "2014-09-05T04:07:33+01:00", "date_accepted": "2014-09-05T04:07:33+01:00", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/ucbclassics_bujc/article/54911/galley/41421/download/" } ] }, { "pk": 63765, "title": "Cover Art", "subtitle": null, "abstract": "Saya Woolfalk, video still from \"The Empathics,\" 2012.", "language": "en", "license": { "name": "", "short_name": "", "text": null, "url": "" }, "keywords": [], "section": "Front Matter", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/0q20j11m", "frozenauthors": [ { "first_name": "Reginald", "middle_name": "", "last_name": "Daniel", "name_suffix": "", "institution": "", "department": "" } ], "date_submitted": "2014-01-30T23:33:27Z", "date_accepted": "2014-01-30T23:33:27Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/jcmrs/article/63765/galley/48968/download/" } ] }, { "pk": 63761, "title": "Critical Mixed Race Studies: New Directions in the Politics of Race and Representation", "subtitle": null, "abstract": "\"Critical Mixed Race Studies: New Directions in the Politics of Race and Representation” was Andrew J. Jolivétte’s keynote address at the inaugural Critical Mixed Race Studies Conference, November 5, 2010, at DePaul University. Jolivétte posits critical mixed-race pedagogy as a model for developingintersectional coalitions across various categories of difference composed of a \"new American majority\" (people of color, queers, women, immigrants, and youth), which was in fact President Barack Obama’s 2012 winning coalition. This shifts racial formation and social change from binary constructions to more multivalent approaches to achieving human rights and social justice. Taken to a logical conclusion, mixed-race pedagogy could also serve as a similar organizing principle for international movements for equity and social justice.", "language": "en", "license": { "name": "", "short_name": "", "text": null, "url": "" }, "keywords": [ { "word": "miscegenation" }, { "word": "intermarriage" }, { "word": "racially mixed people" }, { "word": "mixed heritage" }, { "word": "multiracial identity" }, { "word": "mixed race identity" }, { "word": "mixed race studies" }, { "word": "critical mixed race studies" }, { "word": "multiracial studies" }, { "word": "critical multiracial studies" }, { "word": "postracial" }, { "word": "coalitions." } ], "section": "Article", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/62p3p25p", "frozenauthors": [ { "first_name": "Andrew", "middle_name": "J.", "last_name": "Jolivétte", "name_suffix": "", "institution": "San Francisco State University", "department": "" } ], "date_submitted": "2014-01-27T06:45:17Z", "date_accepted": "2014-01-27T06:45:17Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/jcmrs/article/63761/galley/48964/download/" } ] }, { "pk": 37741, "title": "De Lima, Paolo. Poesía y guerra interna en el Perú (1980-1992): A Study of Poets and Civil War in Peru", "subtitle": null, "abstract": "Review of Poesia y guerra interna en el Peru (2013) by Paolo de Lima", "language": "es", "license": { "name": "Copyright", "short_name": "Copyright", "text": "", "url": "https://escholarship.org/terms" }, "keywords": [], "section": "Reviews", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/4v922342", "frozenauthors": [ { "first_name": "Richard", "middle_name": "", "last_name": "Leonardo", "name_suffix": "", "institution": "Universidad Nacional Mayor de San Marcos", "department": "None" } ], "date_submitted": "2015-04-24T04:05:24+01:00", "date_accepted": "2015-04-24T04:05:24+01:00", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/mester/article/37741/galley/28459/download/" } ] }, { "pk": 41358, "title": "Detection of 16Sr IX phytoplasma (HLB phytoplasma) in Sunn Hemp (Crotalaria juncea) in São Paulo State, Brazil", "subtitle": null, "abstract": "In São Paulo State, besides the occurrence of \nCandidatus\n Liberibacter americanus and \nCa\n. L. asiaticus, a 16Sr IX group phytoplasma was associated with HLB symptoms, indistinguishable from those caused by liberibacters. This phytoplasma is called HLB phytoplasma and was found widespread in citrus orchards, although at low incidence. The same phytoplasma was found in Sunn Hemp (\nCrotalaria juncea\n) in 2008 and witches’-broom was commonly found associated with 16Sr group IX detection. The aim of this work was to assess the phytoplasma diversity in Sunn Hemp with emphasis at the detection of group 16Sr IX phytoplasma and to establish an association between the occurrence of HLB phytoplasma and symptoms. Sunn Hemp samples were harvested close to the blooming period. Plants were selected in the field when showing symptoms common to phytoplasma infection. We employed universal primers do amplify phytoplasmas in general and group specific primers for 16Sr group IX. PCR products were sequenced to allow grouping of phytoplasmas. We identified five phytoplasmas groups in 48 out of 99 Sunn Hemp plants, belonging to phytoplasma groups 16Sr I, III, VII, IX and XV. The most abundant phytoplasma was the group 16Sr IX, present in 70% of the samples, found in central and north São Paulo State. The occurrence of HLB phytoplasma in Sunn Hemp samples, showing 100% of similarity to the citrus phytoplasma, was highly related to virescence and the second most conspicuous symptom for this infection was witches’-broom.", "language": "en", "license": { "name": "Creative Commons Attribution 4.0", "short_name": "CC BY 4.0", "text": "Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.\n\nNo additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.", "url": "https://creativecommons.org/licenses/by/4.0" }, "keywords": [], "section": "Abstracts of Presentations at the 3rd International Research Conference on Huanglongbing", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/04f8q2t4", "frozenauthors": [ { "first_name": "L.", "middle_name": "F.", "last_name": "Bianco", "name_suffix": "", "institution": "Fundecitrus, Araraquara, Brazil", "department": "None" }, { "first_name": "E.", "middle_name": "C.", "last_name": "Martins", "name_suffix": "", "institution": "Fundecitrus, Araraquara, Brazil", "department": "None" }, { "first_name": "D.", "middle_name": "A.B.", "last_name": "Coletti", "name_suffix": "", "institution": "Fundecitrus, Araraquara, Brazil", "department": "None" }, { "first_name": "R.", "middle_name": "S.", "last_name": "Toloy", "name_suffix": "", "institution": "Fundecitrus, Araraquara, Brazil", "department": "None" }, { "first_name": "N.", "middle_name": "A.", "last_name": "Wulff", "name_suffix": "", "institution": "Fundecitrus, Araraquara, Brazil", "department": "None" } ], "date_submitted": "2014-12-20T02:48:17Z", "date_accepted": "2014-12-20T02:48:17Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/iocv_journalcitruspathology/article/41358/galley/30957/download/" } ] }, { "pk": 41239, "title": "Detection of Candidatus Liberibacter asiaticus in Diaphorina citri caught on yellow sticky traps during the winter and summer of Sao Paulo State Brazil", "subtitle": null, "abstract": "The assessment of bacterialiferous Asian citrus psyllid (ACP) frequency is important in epidemiological and management studies because it can be related with the abundance of inoculum sources and with putative new HLB infections. For that, ACP can be collected directly or on yellow sticky traps (YST) commonly used by Brazilian growers to monitor psyllid population. The YST are usually left in the field for 2 weeks after which time YST are visually evaluated for the ACP presence, and if present, the psyllids are removed from the YST and tested by real-time PCR (qPCR) for liberibacter presence. Previous studies in Florida showed that the incidence of Las-positive ACP declined with increasing time on the YST (Irey et al., 2011). Thus, the objective of this work was to determine if time ACP is keep on YST affects qPCR results for Las and if it was related to weather conditions during winter and summer of Araraquara-SP (Brazil). ACP adults from nymphs reared on Las infected trees were placed on YST (BUG-Agentes Biológicos) in the field and 20 samples with 3 individuals were tested after 0, 1, 3, 9, 12 and 15 days. The results were compared with samples directly collected without trap glue. Experiments were done in June, July and August (winter) and in January, February and March (summer). In contrast with previous report in Florida, no difference on the incidence of Las-positive ACP samples was observed up to 15 days on the YST in both seasons.", "language": "en", "license": { "name": "Creative Commons Attribution 4.0", "short_name": "CC BY 4.0", "text": "Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.\n\nNo additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.", "url": "https://creativecommons.org/licenses/by/4.0" }, "keywords": [], "section": "Abstracts of Presentations at the 3rd International Research Conference on Huanglongbing", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/62p7x279", "frozenauthors": [ { "first_name": "I.", "middle_name": "", "last_name": "Sala", "name_suffix": "", "institution": "Fundecitrus, Araraquara, Brazil", "department": "None" }, { "first_name": "E.", "middle_name": "C.", "last_name": "Martins", "name_suffix": "", "institution": "Fundecitrus, Araraquara, Brazil", "department": "None" }, { "first_name": "D.", "middle_name": "A.B.", "last_name": "Coletti", "name_suffix": "", "institution": "Fundecitrus, Araraquara, Brazil", "department": "None" }, { "first_name": "L.", "middle_name": "H.", "last_name": "Montesino", "name_suffix": "", "institution": "Fundecitrus, Araraquara, Brazil", "department": "None" }, { "first_name": "R.", "middle_name": "B.", "last_name": "Bassanezi", "name_suffix": "", "institution": "Fundecitrus, Araraquara, Brazil", "department": "None" }, { "first_name": "N.", "middle_name": "A.", "last_name": "Wulff", "name_suffix": "", "institution": "Fundecitrus, Araraquara, Brazil", "department": "None" }, { "first_name": "D.", "middle_name": "C.", "last_name": "Texeira", "name_suffix": "", "institution": "Fundecitrus, Araraquara, Brazil", "department": "None" } ], "date_submitted": "2014-11-20T00:12:36Z", "date_accepted": "2014-11-20T00:12:36Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/iocv_journalcitruspathology/article/41239/galley/30838/download/" } ] }, { "pk": 41330, "title": "Development of Symptom Expression and Presence of Candidatus Liberibacter asiaticus in Recently Infected, Mature Orange Trees", "subtitle": null, "abstract": "There have been limited studies and documentation of how long a mature Florida orange tree can remain commercially viable after expressing initial symptoms of the HLB disease. This study focuses on understanding the distribution and spread of HLB symptoms on newly symptomatic, mature trees and the association of these symptoms with the presence of the \nCandidatus \nLiberibacter asisticus (Las) bacterium in the various sectors of a tree. The investigation of this process will lead to a better understanding of how long HLB-infected trees can survive under conventional grove practices and, possibly, to better management decisions on tree elimination.", "language": "en", "license": { "name": "Creative Commons Attribution 4.0", "short_name": "CC BY 4.0", "text": "Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.\n\nNo additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.", "url": "https://creativecommons.org/licenses/by/4.0" }, "keywords": [], "section": "Abstracts of Presentations at the 3rd International Research Conference on Huanglongbing", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/98n8m9ts", "frozenauthors": [ { "first_name": "Timothy", "middle_name": "", "last_name": "Gast", "name_suffix": "", "institution": "Southern Gardens Citrus", "department": "None" }, { "first_name": "April", "middle_name": "", "last_name": "Russo", "name_suffix": "", "institution": "Southern Gardens Citrus", "department": "None" } ], "date_submitted": "2014-12-18T19:44:10Z", "date_accepted": "2014-12-18T19:44:10Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/iocv_journalcitruspathology/article/41330/galley/30929/download/" } ] }, { "pk": 41353, "title": "Differentiation of “Candidatus Liberibacter asiaticus” isolates from Brazil, China, and the United States", "subtitle": null, "abstract": "“\nCandidatus \nLiberibacter asiaticus” is associated with citrus Huanglongbing (HLB, yellow shoot disease), a highly destructive disease currently threatening world citrus production. HLB has a long history in China and was found in Brazil in 2004 and U.S.A. in 2005. There is an urgent need to differentiate isolates of “\nCa. \nL. asiaticus” from different geographical regions for effective control of HLB. In this study, isolates of “\nCa. \nL. asiaticus” collected from Brazil, China and the United States were evaluated based on two previously characterized genomic loci, one locus (\ntrn\n1) with variable tandem repeat numbers (TRNs), and the other locus (\nsnp\n1) is characteristic in single nucleotide polymorphisms (SNPs). A total of 299 strains (84 Brazil, 132 China and 83 U.S.) were analyzed. At the \ntrn\n1 locus, “\nCa. \nL. asiaticus” strains were divided into TRN-A and TRN-B groups. TRN-A isolates dominated the China and U.S. populations but were not detected in the Brazil isolates. In contrast, TRN-B dominated the Brazil isolates but occurred at low frequencies in China (3%) and U.S. (6%). SNP Analyses at the \nsnp\n1 locus established Term-A and Term-G groups. Term-A group included all Brazil and China isolates, along with 6% U. S. isolates which were also TRN-B isolates. The remaining (94%) U. S. isolates were in Term-G group. By combining data from the analyses of the two genomic loci, it is shown that the TRN-A:Term A genotype was unique to China, TRN-A:Term-G genotype was unique to U.S., and the TRN-B:Term-A genotype dominated the Brazil isolates. No TRN-B:Term-B isolates were found.", "language": "en", "license": { "name": "Creative Commons Attribution 4.0", "short_name": "CC BY 4.0", "text": "Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.\n\nNo additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.", "url": "https://creativecommons.org/licenses/by/4.0" }, "keywords": [], "section": "Abstracts of Presentations at the 3rd International Research Conference on Huanglongbing", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/78t0x6n6", "frozenauthors": [ { "first_name": "X.", "middle_name": "", "last_name": "Deng", "name_suffix": "", "institution": "Laboratory of Citrus Huanglongbing Research, South China Agricultural University, Guangzhou, Guangdong, People’s Republic of China", "department": "None" }, { "first_name": "J.", "middle_name": "", "last_name": "Chen", "name_suffix": "", "institution": "United States Department of Agriculture, Agricultural Research Services, San Joaquin Valley Agricultural Sciences Center, Parlier, California, U.S.A.", "department": "None" }, { "first_name": "S.", "middle_name": "", "last_name": "Lopes", "name_suffix": "", "institution": "Fundecitrus, São Paulo, Brazil", "department": "None" }, { "first_name": "X.", "middle_name": "", "last_name": "Wang", "name_suffix": "", "institution": "South-West University, Beibei, Chongqing, People’s Republic of China", "department": "None" } ], "date_submitted": "2014-12-20T02:35:44Z", "date_accepted": "2014-12-20T02:35:44Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/iocv_journalcitruspathology/article/41353/galley/30952/download/" } ] }, { "pk": 41254, "title": "Dispersal Behavior of Diaphorina citri Kuwayama (Hemiptera: Psyllidae) Under Laboratory Conditions", "subtitle": null, "abstract": "Asian citrus psyllid (ACP), \nDiaphorina citri\n Kuwayama, is the vector of huanglongbing (HLB), the most devastating disease of citrus worldwide. Knowledge of ACP dispersal behavior in locating host plants may contribute to our understanding of the spread of HLB within and between citrus trees. We conducted research in laboratory to evaluate ACP host plant finding behavior. In a free-choice situation, ACP adults initially settled at equal rates among seedlings of \nRhododendron simsii\n (non host plant for ACP), \nMurraya panciculata\n and \n“\nLugan”\nCitrus reticulata\n. However, at 18 and 42 h after ACP were released, the mean number of adults per plant on \nR. simsii\n was significantly lower than on \nC. reticulata\n and \nM. paniculata\n, respectively. Numbers of adults on \nC. reticulata\n and \nM. paniculata\n remained statistically equivalenty until 90 h after releases. Within a plant, the ratio of ACP adults on new flush shoots did not significantly increased in comparison to other parts of the tree during 1-4 d after the psyllids were released. Even on 7th day after ACP release, about 30 % adults were observed on plant locations other than flushing shoots. The results indicated that ACP adults differentiated between host and non-host plants faster than they differentiated between different parts of a host plant.", "language": "en", "license": { "name": "Creative Commons Attribution 4.0", "short_name": "CC BY 4.0", "text": "Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.\n\nNo additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.", "url": "https://creativecommons.org/licenses/by/4.0" }, "keywords": [], "section": "Abstracts of Presentations at the 3rd International Research Conference on Huanglongbing", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/4q36c4zv", "frozenauthors": [ { "first_name": "Chuanqing", "middle_name": "", "last_name": "Ruan", "name_suffix": "", "institution": "Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China", "department": "None" }, { "first_name": "Bo", "middle_name": "", "last_name": "Liu", "name_suffix": "", "institution": "Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China", "department": "None" }, { "first_name": "Zhenquan", "middle_name": "", "last_name": "Wu", "name_suffix": "", "institution": "Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China", "department": "None" }, { "first_name": "Tao", "middle_name": "", "last_name": "Li", "name_suffix": "", "institution": "Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China", "department": "None" }, { "first_name": "Hanqing", "middle_name": "", "last_name": "Hu", "name_suffix": "", "institution": "Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China", "department": "None" }, { "first_name": "Guocheng", "middle_name": "", "last_name": "Fan", "name_suffix": "", "institution": "Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China", "department": "None" }, { "first_name": "Yongping", "middle_name": "", "last_name": "Duan", "name_suffix": "", "institution": "USDA-ARS, 2001 South Rock Road, Fort Pierce, FL 34945 USA", "department": "None" }, { "first_name": "David", "middle_name": "G.", "last_name": "Hall", "name_suffix": "", "institution": "USDA-ARS, 2001 South Rock Road, Fort Pierce, FL 34945 USA", "department": "None" } ], "date_submitted": "2014-11-25T19:34:29Z", "date_accepted": "2014-11-25T19:34:29Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/iocv_journalcitruspathology/article/41254/galley/30853/download/" } ] }, { "pk": 41259, "title": "Disrupt the bacterial growth in the insect vector to block the transmission of Candidatus Liberibacter asiaticus to citrus, the causal agent of citrus greening disease", "subtitle": null, "abstract": "The genome of \nCandidatus\n Liberibacter asiaticus (CLas) reveals the presence of luxR, encodingLuxR protein, one of a two component cell-to-cell communication system. However, the genome lacks the second component, luxl, that produces acyl-homoserine lactones (AHLs), suggesting that CLas has a solo LuxR system. Interestingly, we detected compounds that may act as AHLs in the insect vector (psyllids) that are healthy or infected with CLas, but not in the citrus plants. This finding suggests that the insect is the AHL source. The fact that CLas forms a biofilm on the surface of the insect gut indicates the presence of a cell-cell communication system. Here the system is solo LuxR. Moreover, we have confirmed the activity of CLas-LuxR by its expression in \nE. coli\n and detection of LuxR-AHL complex. In order to block the vector transmission of CLas, we produced plants that express LuxR. Insects will acquire CLas and luxR. LuxR will compete with the bacteria for binding to AHL and consequently, CLas will not be able to colonize the insect or form biofilm and fails in the transmission. We aim to provide an environmental friendly solution for the most destructive disease in citrus (Huanglongbing) by producing specific LuxR in citrus to interfere with the vector transmission. As an alternative, we also aim to use synthetic molecules that mimic the specific AHL as an application to disrupt CLas transmission from plant to plant by its vector. More AHL in the insect may confuse the bacteria and induce a strong sticky biofilm that hardly releases cells to plants during insect feeding. Accordingly, the transmission from plant to plant will be diminished or blocked.", "language": "en", "license": { "name": "Creative Commons Attribution 4.0", "short_name": "CC BY 4.0", "text": "Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.\n\nNo additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.", "url": "https://creativecommons.org/licenses/by/4.0" }, "keywords": [], "section": "Abstracts of Presentations at the 3rd International Research Conference on Huanglongbing", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/1053363x", "frozenauthors": [ { "first_name": "N.", "middle_name": "", "last_name": "Killiny", "name_suffix": "", "institution": "Citrus research and Education Center, IFAS, University of Florida, Florida, USA", "department": "None" }, { "first_name": "S.", "middle_name": "", "last_name": "Hajeri", "name_suffix": "", "institution": "Citrus research and Education Center, IFAS, University of Florida, Florida, USA", "department": "None" }, { "first_name": "S.", "middle_name": "", "last_name": "Gowda", "name_suffix": "", "institution": "Citrus research and Education Center, IFAS, University of Florida, Florida, USA", "department": "None" }, { "first_name": "M.", "middle_name": "J.", "last_name": "Davis", "name_suffix": "", "institution": "Citrus research and Education Center, IFAS, University of Florida, Florida, USA", "department": "None" } ], "date_submitted": "2014-11-25T20:36:58Z", "date_accepted": "2014-11-25T20:36:58Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/iocv_journalcitruspathology/article/41259/galley/30858/download/" } ] }, { "pk": 59101, "title": "DNA: Building Blocks of Nanotechnology", "subtitle": null, "abstract": "", "language": "en", "license": null, "keywords": [], "section": "Features", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/3jj458tz", "frozenauthors": [ { "first_name": "Alexander", "middle_name": "", "last_name": "Powers", "name_suffix": "", "institution": "University of California, Berkeley", "department": "" } ], "date_submitted": "2014-04-29T02:38:20+01:00", "date_accepted": "2014-04-29T02:38:20+01:00", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/our_bsj/article/59101/galley/45129/download/" } ] }, { "pk": 19644, "title": "Donoso, Isaac y Andrea Gallo, eds. Literatura hispanofilipina actual. Madrid: Verbum, 2011. Impreso. 177 pp.", "subtitle": null, "abstract": "Donoso, Isaac y Andrea Gallo, eds. \nLiteratura hispanofilipina actual\n. Madrid: Verbum, 2011. Impreso. 177 pp.", "language": "en", "license": { "name": "none", "short_name": "none", "text": "", "url": "https://escholarship.org/terms" }, "keywords": [], "section": "Book Reviews", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/7qz9s5c9", "frozenauthors": [ { "first_name": "Paula", "middle_name": "", "last_name": "Park", "name_suffix": "", "institution": "", "department": "None" } ], "date_submitted": "2014-11-05T01:13:29Z", "date_accepted": "2014-11-05T01:13:29Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/transmodernity/article/19644/galley/9731/download/" } ] }, { "pk": 41248, "title": "Early detection surveillance for Huanglongbing in a plantation; from theory to practice", "subtitle": null, "abstract": "The detection of a new HLB epidemic in a planting often occurs when the disease has already reached high incidence. This is problematic and once the epidemic has become established it is difficult to recover the economic productivity of the grove. However, if new epidemics can be detected early enough then more can be done to control the disease. Early detection requires that surveillance surveys be in place before the disease arrives; that is, a number of trees within a healthy grove should be inspected at regular intervals for symptoms of HLB. Exactly how many trees should be surveyed and how frequently this should be done is a non-trivial problem and one that has not previously been addressed in plant pathology. We present a theoretical method that relates the dynamics of an invading epidemic to the dynamics of a monitoring program. The method determines exactly how an early detection survey should be designed in order to achieve a high probability of detecting an epidemic whilst it is at an early stage. We compare the theoretical method to a complex simulation model which replicates the spatial and temporal dynamics of HLB in the field. By running the model thousands of times we can make probabilistic predictions on early detection survey design that can be directly compared with the theoretical method. We find striking similarities between the simple theoretical and more complicated simulation approach that enables us to make valuable new insights as well as deliver methods for transfer into practice.", "language": "en", "license": { "name": "Creative Commons Attribution 4.0", "short_name": "CC BY 4.0", "text": "Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.\n\nNo additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.", "url": "https://creativecommons.org/licenses/by/4.0" }, "keywords": [], "section": "Abstracts of Presentations at the 3rd International Research Conference on Huanglongbing", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/68g7k5sk", "frozenauthors": [ { "first_name": "S.", "middle_name": "", "last_name": "Parnell", "name_suffix": "", "institution": "Rothamsted Research, Harpenden, United Kingdom", "department": "None" }, { "first_name": "T.", "middle_name": "R.", "last_name": "Gottwald", "name_suffix": "", "institution": "USDA, ARS, U.S. Horticultural Research Laboratory, Fort Pierce, FL 34945, USA", "department": "None" }, { "first_name": "N.", "middle_name": "J.", "last_name": "Cunniffe", "name_suffix": "", "institution": "University of Cambridge, Department of Plant Sciences, Cambridge, United Kingdom", "department": "None" }, { "first_name": "F.", "middle_name": "", "last_name": "van den Bosch", "name_suffix": "", "institution": "Rothamsted Research, Harpenden, United Kingdom", "department": "None" } ], "date_submitted": "2014-11-25T19:11:47Z", "date_accepted": "2014-11-25T19:11:47Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/iocv_journalcitruspathology/article/41248/galley/30847/download/" } ] }, { "pk": 41334, "title": "Early root infection and damage in Huanglongbing disease development", "subtitle": null, "abstract": "Huanglongbing in grove trees is initially identified by foliar symptoms, most commonly blotchy mottle. Detection of \nCandidatus\n Liberibacter asiaticus (Las) in leaf tissue by qPCR early in disease development is usually limited to symptomatic leaves and proximal young leaves. Over multiple years, disease symptoms spread to the rest of the canopy. Although Las has been detected in root tissue, the decline of roots has been assumed to happen later in disease development when photosynthate production and transport have been significantly diminished in the tree canopy. Observations of initial spread of Las from the bud-inoculation site in the trunk of 1-yr-old potted trees have revealed that Las is frequently detectable in roots months before detection of Las in leaves and foliar symptom development. Even after symptom development Las is more evenly distributed in root tissue than in the canopy. Preliminary evidence suggests that Las is also more evenly distributed in roots of grove trees. Asymptomatic 9 year old grove trees with root Las infection had 26-41% lower root density than asymptomatic trees without detectable root Las. The loss of root density was independent of Las detection in leaves. Root loss precedes carbohydrate starvation as evidenced by root starch concentrations, suggesting the bacteria may play a more active role in root loss than phloem plugging. These results suggest that early invasion of roots by Las leads to root decline before the appearance of foliar symptoms and is likely the cause of larger than expected yield reduction on trees with limited foliar symptoms.", "language": "en", "license": { "name": "Creative Commons Attribution 4.0", "short_name": "CC BY 4.0", "text": "Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.\n\nNo additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.", "url": "https://creativecommons.org/licenses/by/4.0" }, "keywords": [], "section": "Abstracts of Presentations at the 3rd International Research Conference on Huanglongbing", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/9g12g03c", "frozenauthors": [ { "first_name": "E.", "middle_name": "G.", "last_name": "Johnson", "name_suffix": "", "institution": "UF-CREC, Lake Alfred, Florida, USA", "department": "None" }, { "first_name": "J.", "middle_name": "", "last_name": "Wu", "name_suffix": "", "institution": "UF-CREC, Lake Alfred, Florida, USA", "department": "None" }, { "first_name": "D.", "middle_name": "B.", "last_name": "Bright", "name_suffix": "", "institution": "UF-CREC, Lake Alfred, Florida, USA", "department": "None" }, { "first_name": "J.", "middle_name": "H.", "last_name": "Graham", "name_suffix": "", "institution": "UF-CREC, Lake Alfred, Florida, USA", "department": "None" } ], "date_submitted": "2014-12-19T00:05:30Z", "date_accepted": "2014-12-19T00:05:30Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/iocv_journalcitruspathology/article/41334/galley/30933/download/" } ] }, { "pk": 20974, "title": "Eco-Certification of Natural Rubber: Demand, Supply, and Potential Implications of Private Global Environmental Governance", "subtitle": null, "abstract": "In recent years, concern over the environmental impacts of natural rubber culti- vation has generated considerable interest in eco-certification, a form of private environ- mental regulation designed to encourage more sustainable land-use practices. This paper explores the emergence and potential sovereignty implications of this approach to envi- ronmental control with an emphasis on the natural rubber industry. I argue that although eco-certification is advocated as a form of networked governance representing a range of political interests, the way certification programs position themselves as transparent and accountable alternatives to state-based regulation potentially serves to delegitimize the role of the state in the arena of environmental regulation.", "language": "en", "license": { "name": "none", "short_name": "none", "text": "", "url": "https://escholarship.org/terms" }, "keywords": [], "section": "Article", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/02d3918r", "frozenauthors": [ { "first_name": "Sean", "middle_name": "", "last_name": "Kennedy", "name_suffix": "", "institution": "UCLA", "department": "None" } ], "date_submitted": "2014-11-23T23:57:00Z", "date_accepted": "2014-11-23T23:57:00Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/criticalplanning/article/20974/galley/10676/download/" } ] }, { "pk": 19633, "title": "Ecos de poetas hispánicos en los versos de José Rizal", "subtitle": null, "abstract": "Ecos de poetas hispánicos en los versos de José Rizal", "language": "en", "license": { "name": "none", "short_name": "none", "text": "", "url": "https://escholarship.org/terms" }, "keywords": [], "section": "Article", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/40j9h77j", "frozenauthors": [ { "first_name": "Brooke F.", "middle_name": "", "last_name": "Cadwallader Dóndiz y Clavería", "name_suffix": "", "institution": "", "department": "None" } ], "date_submitted": "2014-11-04T18:45:12Z", "date_accepted": "2014-11-04T18:45:12Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/transmodernity/article/19633/galley/9720/download/" } ] }, { "pk": 41246, "title": "Edge Effects and Huanglongbing", "subtitle": null, "abstract": "Huanglongbing (HLB), spread by a psyllid vector, is globally considered a major threat to commercial and sustainable citrus production. Better understanding of the vector-mediated patterns of HLB spread is essential to inform and maximize disease management. From previous studies, edge effects are a significant characteristic of the HLB pathosystem and have been observed predominately in larger plantings. In this study, we investigated 1) the impact of different edge classes and orientations, 2) the quantitative influence of distance from edges, and 3) the temporal dynamics of each edge effect. Spatial analyses of edge effects were conducted on two years of HLB incidence data from the Southern Gardens plantation in south Florida.", "language": "en", "license": { "name": "Creative Commons Attribution 4.0", "short_name": "CC BY 4.0", "text": "Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.\n\nNo additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.", "url": "https://creativecommons.org/licenses/by/4.0" }, "keywords": [], "section": "Abstracts of Presentations at the 3rd International Research Conference on Huanglongbing", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/5hf0d0s3", "frozenauthors": [ { "first_name": "W.", "middle_name": "", "last_name": "Luo", "name_suffix": "", "institution": "USDA, ARS, US Horticultural Research Laboratory, Fort Pierce, Florida, USA;\nCIPM, NC State University, Raleigh, North Carolina, USA", "department": "None" }, { "first_name": "D.", "middle_name": "J.", "last_name": "Anco", "name_suffix": "", "institution": "USDA, ARS, US Horticultural Research Laboratory, Fort Pierce, Florida, USA;\nCIPM, NC State University, Raleigh, North Carolina, USA", "department": "None" }, { "first_name": "T.", "middle_name": "R.", "last_name": "Gottwald", "name_suffix": "", "institution": "USDA, ARS, US Horticultural Research Laboratory, Fort Pierce, Florida, USA;", "department": "None" }, { "first_name": "M.", "middle_name": "S.", "last_name": "Irey", "name_suffix": "", "institution": "Southern Gardens Citrus, US Sugar Corp., Clewiston, Florida, USA", "department": "None" } ], "date_submitted": "2014-11-25T18:48:40Z", "date_accepted": "2014-11-25T18:48:40Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/iocv_journalcitruspathology/article/41246/galley/30845/download/" } ] }, { "pk": 56464, "title": "Editorial", "subtitle": null, "abstract": "[no abstract]", "language": "en", "license": null, "keywords": [], "section": "Editorial", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/0r14j843", "frozenauthors": [ { "first_name": "Nana", "middle_name": "", "last_name": "Osei-Opare", "name_suffix": "", "institution": "UCLA", "department": "" }, { "first_name": "Jeremy", "middle_name": "Jacob", "last_name": "Peretz", "name_suffix": "", "institution": "UCLA", "department": "" } ], "date_submitted": "2014-12-14T03:32:30Z", "date_accepted": "2014-12-14T03:32:30Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/ufahamu/article/56464/galley/42872/download/" } ] }, { "pk": 63211, "title": "Editors' Introduction", "subtitle": null, "abstract": "Introduction to Volume 4, Issue 2.", "language": "en", "license": null, "keywords": [ { "word": "Education" } ], "section": "Editors' Introduction", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/0g93m2qd", "frozenauthors": [ { "first_name": "BRE", "middle_name": "", "last_name": "Editors", "name_suffix": "", "institution": "University of California, Berkeley", "department": "" } ], "date_submitted": "2014-02-28T00:35:19Z", "date_accepted": "2014-02-28T00:35:19Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/bre/article/63211/galley/48785/download/" } ] }, { "pk": 63752, "title": "Editor's Note", "subtitle": null, "abstract": "Journal of Critical Mixed Race Studies \n(JCMRS) is a new scholarly outlet that seeks to bring together innovative work on the topic of mixed race in the United States and abroad.", "language": "en", "license": { "name": "", "short_name": "", "text": null, "url": "" }, "keywords": [], "section": "Front Matter", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/5sg4b35k", "frozenauthors": [ { "first_name": "G.", "middle_name": "Reginald", "last_name": "Daniel", "name_suffix": "", "institution": "University of California, Santa Barbara", "department": "" } ], "date_submitted": "2014-01-27T01:35:35Z", "date_accepted": "2014-01-27T01:35:35Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/jcmrs/article/63752/galley/48956/download/" } ] }, { "pk": 6044, "title": "Education & Incarceration: How Their Intersection Affects a Latino/a Household", "subtitle": null, "abstract": "Education & Incarceration: How Their Intersection Affects a Latino/a Household", "language": "en", "license": { "name": "All rights reserved", "short_name": "Copyright", "text": "© the author(s). All rights reserved.", "url": "https://creativecommons.org/licenses/authors" }, "keywords": [], "section": "Articles", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/6f79486k", "frozenauthors": [ { "first_name": "Wendy", "middle_name": "", "last_name": "Hernandez", "name_suffix": "", "institution": "", "department": "None" } ], "date_submitted": "2014-11-23T05:33:02Z", "date_accepted": "2014-11-23T05:33:02Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "", "path": "https://journalpub.escholarship.org/our_buj/article/6044/galley/3682/download/" } ] }, { "pk": 41318, "title": "Effect of beneficial bacterial isolates from citrus roots in Florida on citrus Huanglongbing disease development", "subtitle": null, "abstract": "Huanglongbing (HLB) is the most devastating disease of citrus in Florida. HLB is caused by the phloem-inhabiting bacterium ‘\nCandidatus \nLiberibacter asiaticus’ (Las), which is transmitted by psyllid vector \nDiaphorina citri\n. The current management strategies of HLB are to control psyllids and eradicate infected plants. However, these management practices have not been able to stop the spreading of HLB (Duan et al. 2009). Alternative approaches are needed to control HLB. In previous studies we isolated multiple bacterial strains from roots of healthy citrus plants in HLB infected groves in Florida, which have the potential to enhance plant growth and suppress diseases (Trivedi et al. 2011). Recently, early infection of roots by Las leading to root decline was suggested to be important in HLB disease development (Johnson et al. 2012). We hypothesize that introduction of beneficial bacteria to roots of citrus plants could decrease root damage by promoting root growth and reducing Las infection and thus improve HLB management. To test this hypothesis, six beneficial bacteria were assessed for their plant growth-promotion ability; and three were found to be able to promote growth of both grapefruit and \nArabidopsis\n (Col-0 ecotype) in greenhouse experiments, with increases in root length and weight, compared to mock treated plants. Thus, the three isolates were selected to evaluate the effect on Las infection in greenhouse assays. Details of the beneficial bacterial isolates, plant growth-promotion activity, and effect on Las infection will be discussed.", "language": "en", "license": { "name": "Creative Commons Attribution 4.0", "short_name": "CC BY 4.0", "text": "Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.\n\nNo additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.", "url": "https://creativecommons.org/licenses/by/4.0" }, "keywords": [], "section": "Abstracts of Presentations at the 3rd International Research Conference on Huanglongbing", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/8f6364rt", "frozenauthors": [ { "first_name": "J.", "middle_name": "", "last_name": "Li", "name_suffix": "", "institution": "Citrus Research and Education Center, Department of Microbiology and Cell Science, University of Florida, Lake Alfred, FL, 33850 USA", "department": "None" }, { "first_name": "P.", "middle_name": "", "last_name": "Trivedi", "name_suffix": "", "institution": "Citrus Research and Education Center, Department of Microbiology and Cell Science, University of Florida, Lake Alfred, FL, 33850 USA", "department": "None" }, { "first_name": "N.", "middle_name": "", "last_name": "Wang", "name_suffix": "", "institution": "Citrus Research and Education Center, Department of Microbiology and Cell Science, University of Florida, Lake Alfred, FL, 33850 USA", "department": "None" } ], "date_submitted": "2014-12-17T22:48:14Z", "date_accepted": "2014-12-17T22:48:14Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/iocv_journalcitruspathology/article/41318/galley/30917/download/" } ] }, { "pk": 41324, "title": "Effect of Enhanced Zinc Nutrition on Mitigation of Huanglongbing (HLB)-affected Citrus", "subtitle": null, "abstract": "The growth decline of huanglongbing (HLB)-affected citrus trees is considered to be associated with nutritional disorder, as typical symptoms of HLB such as stunted tree growth, chlorosis or blotchy mottle of leaves, resembles zinc (Zn), iron (Fe) and manganese (Mn) deficiencies, while lower Zn concentration has been consistently reported in the HLB affected compared to healthy plants. Hydroponic culture studies were conducted to evaluate the effects of enhanced Zn nutrition on the mitigation of HLB-affected grapefruit seedlings. Modified Hoagland nutrient solutions were used with three Zn2+ levels: 0, 1.0, and 1.5 times that of standard strength. Both HLB-affected and healthy grapefruit seedlings were subjected to the treatments for 49 days. During the growth period, photosynthesis of plant leaves was measured, and at day 49 of the culture, leaf tissues and cells were examined for structural changes using light and scanning electron microscopy. Enhanced Zn nutrition generally improved the growth of HLB plants with less symptom severity. Photosynthesis, in terms of leaf electron transfer rate and photochemical quantum yield, was enhanced. The wax layer and cuticle increased, and the epidermis cells became better organized, with a higher number of normal stomatal openings, compared to the control. In addition, enhanced Zn nutrition resulted in more developed xylem and phloem transport systems, resulting in reduced starch grains and polyphenol substances in the leaf cells. These results indicate that\n \nenhanced Zn nutrition can improve the structure of photosynthetic, transfusion and protective tissue systems, and thus promote photosynthesis, transport of photosynthates, and other related metabolism of HLB-infected citrus.", "language": "en", "license": { "name": "Creative Commons Attribution 4.0", "short_name": "CC BY 4.0", "text": "Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.\n\nNo additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.", "url": "https://creativecommons.org/licenses/by/4.0" }, "keywords": [], "section": "Abstracts of Presentations at the 3rd International Research Conference on Huanglongbing", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/1pv647wv", "frozenauthors": [ { "first_name": "S.", "middle_name": "L.", "last_name": "Li", "name_suffix": "", "institution": "University of Florida, IFAS, Indian River REC, Fort Pierce, USA", "department": "None" }, { "first_name": "Z.", "middle_name": "G.", "last_name": "Li", "name_suffix": "", "institution": "University of Florida, IFAS, Indian River REC, Fort Pierce, USA", "department": "None" }, { "first_name": "Z.", "middle_name": "L.", "last_name": "He", "name_suffix": "", "institution": "University of Florida, IFAS, Indian River REC, Fort Pierce, USA", "department": "None" } ], "date_submitted": "2014-12-17T23:11:16Z", "date_accepted": "2014-12-17T23:11:16Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/iocv_journalcitruspathology/article/41324/galley/30923/download/" } ] }, { "pk": 41277, "title": "Effect of Mineral Oil on Host Selection and Control of Diaphorina citri Kuwayama (Hemiptera: Psillidae) on Citrus", "subtitle": null, "abstract": "This research was carried out to study the influence of mineral oil on landing and permanence; oviposition; and mortality of \nDiaphorina citri\n Kuwayama (Hemiptera: Psyllidae) on citrus plants. For all experiments, mineral oil (Argenfrut®) was sprayed on sweet orange plants at 1% concentration. Landing-permanence and oviposition were assessed using choice and non-choice tests. For the first parameter, 50 adult psyllids were released in the center of the screen house (5mx2.5mx2m) (n = 10) and the number of psyllids/plant at different time intervals were counted. For the oviposition trial, which was conducted under laboratory conditions, 20 psyllids were confined in a cage to oviposit (n = 10) and after three days the number of eggs/plant were counted. The effect of mineral oil on psyllid mortality was assessed by confining 10 adult psyllids per plant (n=4) with fully expanded mature leaves after spray. Assessment was conducted 7 days after confining the insects, by determining the number of live and dead psyllids. The results of this research indicate that mineral oil has repellent effect on adults of \nD. citri\n, which prefer oil-free plants to land, remain and oviposit. Moreover, mineral oil was effective on \nD. citri \ncontrol (mortality≥80%).", "language": "en", "license": { "name": "Creative Commons Attribution 4.0", "short_name": "CC BY 4.0", "text": "Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.\n\nNo additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.", "url": "https://creativecommons.org/licenses/by/4.0" }, "keywords": [], "section": "Abstracts of Presentations at the 3rd International Research Conference on Huanglongbing", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/9dc2215g", "frozenauthors": [ { "first_name": "M.", "middle_name": "P.", "last_name": "Miranda", "name_suffix": "", "institution": "Fundecitrus, Araraquara, Brazil", "department": "None" }, { "first_name": "M.", "middle_name": "L.", "last_name": "Micelli", "name_suffix": "", "institution": "Fundecitrus, Araraquara, Brazil", "department": "None" }, { "first_name": "M.", "middle_name": "R.", "last_name": "Felippe", "name_suffix": "", "institution": "Fundecitrus, Araraquara, Brazil", "department": "None" }, { "first_name": "R.", "middle_name": "E.", "last_name": "Caldeira", "name_suffix": "", "institution": "Fundecitrus, Araraquara, Brazil", "department": "None" }, { "first_name": "P.", "middle_name": "T.", "last_name": "Yamamoto", "name_suffix": "", "institution": "ESALQ/Universidade de São Paulo, Piracicaba, Brazil", "department": "None" } ], "date_submitted": "2014-12-16T22:28:30Z", "date_accepted": "2014-12-16T22:28:30Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/iocv_journalcitruspathology/article/41277/galley/30876/download/" } ] }, { "pk": 41236, "title": "Effect of Temperature on Lighted Sticky Traps (TransTrap®) used to Detect Asian Citrus Psyllids in Shipping Containers", "subtitle": null, "abstract": "The Asian citrus psyllid (ACP) is an insect native to tropical and subtropical regions in Asia, which has spread into most citrus producing regions around the world. The main concern with ACP is the spread of Huanglongbing (HLB), a disease vectored by the psyllid. The volume of citrus shipments from Mexico into the U.S. prompted questions about the risk of moving ACP and HLB on this pathway. Our goal was to determine temperatures at which ACP would be active and thus fly to lighted sticky traps inside a transport trailer.\n \nOur results found that temperature significantly affected the number of adults captured. No psyllids were recovered on the traps at 12˚C (53˚F), which is the average temperature of citrus shipments crossing the border. This is compare with an average capture of 43, 47, and 42% of the released adults at 24, 28, and 32°C (75, 82, and 90°F), respectively. Only 1%, 2% and 18% were captured at 18, 20 and 22°C (64, 68, and 72˚F), respectively. Given that the average temperature of limes arriving at the border is 12˚C, it is unlikely that ACP adults would fly to a lighted sticky trap placed inside a refrigerated trailer. In addition, the majority of the packing houses in Mexico place the fruit into coolers prior to loading. We believe a lighted sticky trap would not be effective in detecting ACP within refrigerated citrus shipments for the period of time from loading at the packing house to trap recovery at the border crossing.", "language": "en", "license": { "name": "Creative Commons Attribution 4.0", "short_name": "CC BY 4.0", "text": "Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.\n\nNo additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.", "url": "https://creativecommons.org/licenses/by/4.0" }, "keywords": [], "section": "Abstracts of Presentations at the 3rd International Research Conference on Huanglongbing", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/43n666nv", "frozenauthors": [ { "first_name": "David", "middle_name": "", "last_name": "Bartels", "name_suffix": "", "institution": "USDA APHIS PPQ CPHST Mission Lab, Edinburg, TX", "department": "None" }, { "first_name": "Jason", "middle_name": "", "last_name": "Carlson", "name_suffix": "", "institution": "USDA APHIS PPQ CPHST Mission Lab, Edinburg, TX", "department": "None" }, { "first_name": "Matt", "middle_name": "", "last_name": "Ciomperlik", "name_suffix": "", "institution": "USDA APHIS PPQ CPHST Mission Lab, Edinburg, TX", "department": "None" } ], "date_submitted": "2014-11-20T00:01:39Z", "date_accepted": "2014-11-20T00:01:39Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/iocv_journalcitruspathology/article/41236/galley/30835/download/" } ] }, { "pk": 41237, "title": "Effect of time and storage methods on the detection of Candidatus Liberibacter asiaticus in Diaphorina citri by qPCR", "subtitle": null, "abstract": "The assessment of bacterialiferous Asian citrus psyllid (ACP) frequency is important for (i) studies of bacteria acquisition and inoculation by ACP, (ii) disease detection in disease free areas but with ACP presence, (iii) efficiency evaluation of inoculum reduction strategies, (iv) evaluation of frequency of \nCandidatus \nLiberibacter asiaticus (Las)-positive ACP and the abundance of inoculum sources or putative new HLB infections relationships. Depending on the conditions and time of storage of collected psyllids, Las DNA in ACP could degrade and Las-false negative results might occur. Thus, this study was conducted to evaluate the detection of Las in ACP adults submitted to different storage methods and time of storage by real-time PCR (qPCR). Two 2x3x7 factorial experiments were conducted. Factors were ‘Ethanol’ (with or without 70% ethanol), ‘Temperature’ (-20°C, 4°C and 26°C) and ‘Time’ (0, 3, 7, 14, 21, 28 and 35 days). For each treatment, 20 samples with 3 ACP adults from nymphs reared on Las infected trees were tested for Las presence by qPCR. No significant differences in percentages of psyllids samples positive for Las were observed among the storage methods up to 35 days, except a slight trend of decline in Las detection in samples storage without ethanol at 26°C after 14 days of storage.", "language": "en", "license": { "name": "Creative Commons Attribution 4.0", "short_name": "CC BY 4.0", "text": "Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.\n\nNo additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.", "url": "https://creativecommons.org/licenses/by/4.0" }, "keywords": [], "section": "Abstracts of Presentations at the 3rd International Research Conference on Huanglongbing", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/86v5p9sb", "frozenauthors": [ { "first_name": "I.", "middle_name": "", "last_name": "Sala", "name_suffix": "", "institution": "Fundecitrus, Araraquara, Brazil", "department": "None" }, { "first_name": "E.", "middle_name": "C.", "last_name": "Martins", "name_suffix": "", "institution": "Fundecitrus, Araraquara, Brazil", "department": "None" }, { "first_name": "D.", "middle_name": "A.B.", "last_name": "Coletti", "name_suffix": "", "institution": "Fundecitrus, Araraquara, Brazil", "department": "None" }, { "first_name": "L.", "middle_name": "H.", "last_name": "Montesino", "name_suffix": "", "institution": "Fundecitrus, Araraquara, Brazil", "department": "None" }, { "first_name": "R.", "middle_name": "B.", "last_name": "Bassanezi", "name_suffix": "", "institution": "Fundecitrus, Araraquara, Brazil", "department": "None" }, { "first_name": "N.", "middle_name": "A.", "last_name": "Wulff", "name_suffix": "", "institution": "Fundecitrus, Araraquara, Brazil", "department": "None" }, { "first_name": "D.", "middle_name": "C.", "last_name": "Teixeira", "name_suffix": "", "institution": "Fundecitrus, Araraquara, Brazil", "department": "None" } ], "date_submitted": "2014-11-20T00:03:49Z", "date_accepted": "2014-11-20T00:03:49Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/iocv_journalcitruspathology/article/41237/galley/30836/download/" } ] }, { "pk": 41284, "title": "Effects of Tagetes coronopifolia and T. lemmonii (Asteraceae) essential oils in nymphs of Diaphorina citri (Hemiptera: Psyllidae)", "subtitle": null, "abstract": "Diaphorina citri Kuwayama (Hemiptera: Psyllidae) is performed mainly by the use of agrochemicals. Often, indiscriminate use of insecticides has resulted in the elimination of natural enemies and in the development of insecticide resistance in the target pest and in no target insects. This situation has motivated the generation and implementation of alternative strategies, such as the use of insecticidal plants. This study was carried out to evaluate the toxic effect of \nTagetes coronopifolia\n Willd.\n \nand \nT. lemmonii \nA. Gray (Asteraceae) essential oils in \nD. citri\n third\n \ninstar nymphs. Effect was evaluated by orange disc immersion method. Mortality was recorded 24 hours after applying the oils; the log dose response line Probit and the LC50 values were determined. \nT. coronopifolia \nand \nT. lemmonni \noils were toxic to \nD. citri \nnymphs and the effect was positively related to the concentration. Highest nymph mortality (≥ 98%) with both oils was registered in concentrations of 10 mg mL-1. The LC50 estimated for \nT. lemmonii \nand \nT. coronopifolia \noils was 0.034 and 0.094 mg mL-1, respectively.", "language": "en", "license": { "name": "Creative Commons Attribution 4.0", "short_name": "CC BY 4.0", "text": "Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.\n\nNo additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.", "url": "https://creativecommons.org/licenses/by/4.0" }, "keywords": [], "section": "Abstracts of Presentations at the 3rd International Research Conference on Huanglongbing", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/2z22z5n9", "frozenauthors": [ { "first_name": "E.", "middle_name": "E.", "last_name": "Mendoza-García", "name_suffix": "", "institution": "Colegio de Postgraduados, Campus Montecillo, Entomología. Montecillo, Estado de México, 56230", "department": "None" }, { "first_name": "L.", "middle_name": "D.", "last_name": "Ortega-Arenas", "name_suffix": "", "institution": "Colegio de Postgraduados, Campus Montecillo, Entomología. Montecillo, Estado de México, 56230", "department": "None" }, { "first_name": "M.", "middle_name": "A.", "last_name": "Serrato-Cruz", "name_suffix": "", "institution": "Universidad Autónoma Chapingo-Agroecología, Chapingo, Estado de México, 56230", "department": "None" }, { "first_name": "J.", "middle_name": "A.", "last_name": "Villanueva-Jiménez", "name_suffix": "", "institution": "Colegio de Postgraduados, Campus Montecillo, Entomología. Montecillo, Estado de México, 56230", "department": "None" }, { "first_name": "J.", "middle_name": "I.", "last_name": "López-Arroyo", "name_suffix": "", "institution": "INIFAP, Centro de Investigación Regional del Noreste. Río Bravo, Tam., México, 88900", "department": "None" }, { "first_name": "R.", "middle_name": "", "last_name": "Pérez-Pacheco", "name_suffix": "", "institution": "CIIDIR-OAXACA-IPN. Xoxocotlán, Oaxaca, México, 71230. mendoza.edgar@colpos.mx", "department": "None" } ], "date_submitted": "2014-12-16T23:43:29Z", "date_accepted": "2014-12-16T23:43:29Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/iocv_journalcitruspathology/article/41284/galley/30883/download/" } ] }, { "pk": 41234, "title": "Efficiency of different set of primers in PCR detection of 'Candidatus Liberibacter asiaticus’", "subtitle": null, "abstract": "Huanglongbing (HLB), caused by the bacteria ‘\nCandidatus \nLiberibacter spp’, is one of the most destructive diseases for citrus production around the world. Early diagnosis of diseased citrus trees is of utmost importance for the control of HLB. Molecular techniques, such as PCR, offer quick and specific detection and identification of ‘\nCa\n. Liberibacter spp.’. Furthermore, different sets of primers have been used for detection of ‘\nCa\n. Liberibacter asiaticus’, the main agent of HLB. However, neither of the described sets of primers may detect all cases of HLB. Thus, conventional PCR using three common sets of primers, A2/J5, OI1/OI2c, and Lp1c/HP1, which amplify fragments of 703 pb, 1200 pb, and 2400 pb, respectively, were examined for HLB diagnosis. A total of 969 samples from different citrus cultivars, tree ages and regions of the State of Paraná, Brazil, were included in this study. The total number of samples tested positive for HLB by any set of primers were 598, representing 61.7% of the samples examined. Among these positive samples, 96.7% were identified with the primers A2/J5, 86.6% with OI2c/OI1, and 81.4% with HP1/Lp1c. When combined, 99.8% of the samples were HLB positive based on the sets of primers A2/J5 and OI2c/OI1. Based on the results obtained, the set of primers A2/J5 showed the highest efficiency in the detection of HLB for the bacterium that occurs in the State of Parana, Brazil.", "language": "en", "license": { "name": "Creative Commons Attribution 4.0", "short_name": "CC BY 4.0", "text": "Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.\n\nNo additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.", "url": "https://creativecommons.org/licenses/by/4.0" }, "keywords": [], "section": "Abstracts of Presentations at the 3rd International Research Conference on Huanglongbing", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/4vw2q5tb", "frozenauthors": [ { "first_name": "L.", "middle_name": "", "last_name": "Meneguim", "name_suffix": "", "institution": "IAPAR, Área de Proteção de Plantas, Londrina, Paraná, Brazil", "department": "None" }, { "first_name": "A.", "middle_name": "L.", "last_name": "Naldi", "name_suffix": "", "institution": "UNIFIL, Centro Universitário Filadelfia, Londrina, Paraná, Brazil", "department": "None" }, { "first_name": "C.", "middle_name": "D.", "last_name": "Poças", "name_suffix": "", "institution": "", "department": "None" }, { "first_name": "R.", "middle_name": "P.", "last_name": "Leite Júnior", "name_suffix": "", "institution": "IAPAR, Área de Proteção de Plantas, Londrina, Paraná, Brazil", "department": "None" } ], "date_submitted": "2014-11-19T23:55:38Z", "date_accepted": "2014-11-19T23:55:38Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/iocv_journalcitruspathology/article/41234/galley/30833/download/" } ] }, { "pk": 19629, "title": "El privilegio de subvertir: la literatura hispanofilipina", "subtitle": null, "abstract": "El privilegio de subvertir: la literatura hispanofilipina", "language": "en", "license": { "name": "none", "short_name": "none", "text": "", "url": "https://escholarship.org/terms" }, "keywords": [], "section": "Article", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/7f89w1jn", "frozenauthors": [ { "first_name": "Beatriz", "middle_name": "", "last_name": "Álvarez Tardío", "name_suffix": "", "institution": "", "department": "None" } ], "date_submitted": "2014-11-04T18:31:58Z", "date_accepted": "2014-11-04T18:31:58Z", "date_published": "2014-01-01T00:00:00Z", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/transmodernity/article/19629/galley/9716/download/" } ] } ] }