Article Instance
API Endpoint for journals.
GET /api/articles/41222/?format=api
{ "pk": 41222, "title": "Detection of Liberibacter asiaticus in a single infected Asian citrus psyllid adult or nymph: Impact of dilution with clean Asian citrus psyllids (Diaphorina citri) during extraction", "subtitle": null, "abstract": "Now that the presence of Huanglongbing (HLB) has been confirmed in California, protecting the state’s citrus industry through early detection of disease is essential in curtailing its spread. Because ‘\nCandidatus\n Liberibacter asiaticus’ (Las), the putative causal agent of HLB, accumulates in its vector, the Asian citrus psyllid (ACP), \nDiaphorina citri\n, Kuwayama, accurate testing of the insect is vital. Due to the fact that insect secondary metabolites interfere with downstream applications (1, 3) there is concern about the number of insects pooled in DNA extractions without compromising Las detection. The current USDA CPHST approved method of testing (2) limits the pooling size to five individual insects per extraction followed by the highly sensitive quantitative polymerase chain reaction (QPCR) detection technique. The QPCR reaction targets a region of the Las 16S ribosomal gene (4) and simultaneously the \nD. citri\n specific \nwingless\n (WGLS) gene (5) as an internal control for extraction efficiency. In contrast, the CDFA laboratory routinely pools up to 25 individual insects for DNA extraction, followed by the same QPCR detection. If pooling 25 individuals is indeed a safe practice all the labs currently limiting sample pooling to five individuals could save a substantial amount of time and money. We decided to approximate the pooling limit using single insect equivalents of DNA extracted from Las infected ACP from colonies at USDA-ARS in Fort Pierce, Florida pooled with intact ACP from clean UC Riverside, California, quarantine colonies. Depending on results we would follow these experiments with experiments using intact Las positive ACP.", "language": "en", "license": { "name": "Creative Commons Attribution 4.0", "short_name": "CC BY 4.0", "text": "Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.\n\nNo additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.", "url": "https://creativecommons.org/licenses/by/4.0" }, "keywords": [], "section": "Abstracts of Presentations at the 3rd International Research Conference on Huanglongbing", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/75x3b2jj", "frozenauthors": [ { "first_name": "Cynthia", "middle_name": "", "last_name": "LeVesque", "name_suffix": "", "institution": "California Citrus Research Board and Citrus Pest and Disease Prevention Program Jerry Dimitman Laboratory, 1201 Research Park Dr., Suite 500, Riverside, CA 92507", "department": "None" }, { "first_name": "Lucita", "middle_name": "", "last_name": "Kumagai", "name_suffix": "", "institution": "Plant Pest Diagnostics Branch, California Department of Food & Agriculture, 3294 Meadowview Rd., Sacramento, CA 95832", "department": "None" }, { "first_name": "Cheryl", "middle_name": "", "last_name": "Bloomquist", "name_suffix": "", "institution": "Plant Pest Diagnostics Branch, California Department of Food & Agriculture, 3294 Meadowview Rd., Sacramento, CA 95832", "department": "None" }, { "first_name": "Manjunath", "middle_name": "", "last_name": "Keremane", "name_suffix": "", "institution": "USDA-ARS National Clonal Germplasm Repository for Citrus & Dates, 1060 Martin Luther King Blvd., Riverside CA 92507", "department": "None" }, { "first_name": "Richard", "middle_name": "", "last_name": "Lee", "name_suffix": "", "institution": "USDA-ARS National Clonal Germplasm Repository for Citrus & Dates, 1060 Martin Luther King Blvd., Riverside CA 92507", "department": "None" }, { "first_name": "Madhurababu", "middle_name": "", "last_name": "Kunta", "name_suffix": "", "institution": "Plant Disease Diagnostic Laboratory, TAMUK Citrus Center, 312 N. International Blvd., Weslaco, TX 78596", "department": "None" }, { "first_name": "John", "middle_name": "V.", "last_name": "da Graça", "name_suffix": "", "institution": "Plant Disease Diagnostic Laboratory, TAMUK Citrus Center, 312 N. International Blvd., Weslaco, TX 78596", "department": "None" }, { "first_name": "Georgious", "middle_name": "", "last_name": "Vidalakis", "name_suffix": "", "institution": "Citrus Clonal Protection Program, Dept. of Plant Pathol;ogy, University of California, Riverside, CA 92521", "department": "None" }, { "first_name": "Hong", "middle_name": "", "last_name": "Lin", "name_suffix": "", "institution": "USDA-ARS, 9611 S. Riverbend Ave., Parlier, CA 936489", "department": "None" }, { "first_name": "John", "middle_name": "", "last_name": "Morgan", "name_suffix": "", "institution": "California Citrus Research Board and Citrus Pest and Disease Prevention Program Jerry Dimitman Laboratory, 1201 Research Park Dr., Suite 500, Riverside, CA 92507", "department": "None" }, { "first_name": "David", "middle_name": "G.", "last_name": "Hall", "name_suffix": "", "institution": "USDA-ARS, 2001 South Rock Rd., Fort Pierce, FL 34945", "department": "None" }, { "first_name": "MaryLou", "middle_name": "", "last_name": "Polek", "name_suffix": "", "institution": "California Citrus Research Board, 217 N. Encina St., Visalia, CA 93279", "department": "None" } ], "date_submitted": "2014-10-08T21:01:34-04:00", "date_accepted": "2014-10-08T21:01:34-04:00", "date_published": "2014-11-19T18:27:20-05:00", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/iocv_journalcitruspathology/article/41222/galley/30821/download/" } ] }