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{
    "pk": 41227,
    "title": "Tree-side Molecular Testing for DNA from the HLB Bacterium Candidatus Liberibacter asiaticus",
    "subtitle": null,
    "abstract": "HLB affects all citrus cultivars and causes tree decline resulting in greatly reduced citrus production in Asia, Africa, the Indian subcontinent, the Arabian Peninsula, Brazil, and the United States. To enable integrated pest management strategies for combating HLB, we have developed a sensitive nucleic acid-based HLB diagnostic test based on Mesa Tech International, Inc.’s (MTI's) new point-of-use diagnostic platform, MTIDx, and targeting hyvI/hyvII genes of \nCandidatus\n Liberibacter asiaticus (Las) (Zhou et al., 2011). The MTIDx platform integrates sample preparation, rapid nucleic acid amplification and sequence-specific hybridization-based detection. The simplicity of the test may offer end users with little or no specialized training the opportunity to obtain molecular test results comparable to laboratory-based PCR methods without costly instrumentation. Here, we will report on the efficacy of MTI's nucleic acid testing platform for the detection of \nCandidatus\n Liberibacter asiaticus bacteria in greenhouse and field collected citrus tissue and in the insect vector of the pathogen (\nDiaphorina citri\n Kuwayama). Comparative studies evaluating the MTIDx platform's performance relative to widely accepted laboratory PCR testing will be presented. Results from these studies will be discussed in the context of their implications for HLB management.",
    "language": "en",
    "license": {
        "name": "Creative Commons Attribution 4.0",
        "short_name": "CC BY 4.0",
        "text": "Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.\n\nNo additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.",
        "url": "https://creativecommons.org/licenses/by/4.0"
    },
    "keywords": [],
    "section": "Abstracts of Presentations at the 3rd International Research Conference on Huanglongbing",
    "is_remote": true,
    "remote_url": "https://escholarship.org/uc/item/2165m6zn",
    "frozenauthors": [
        {
            "first_name": "R. Bruce",
            "middle_name": "",
            "last_name": "Cary",
            "name_suffix": "",
            "institution": "Mesa Tech International, Inc., 6404 Nancy Ridge Dr. San Diego, CA 92121",
            "department": "None"
        },
        {
            "first_name": "Hong",
            "middle_name": "",
            "last_name": "Cai",
            "name_suffix": "",
            "institution": "Mesa Tech International, Inc., 6404 Nancy Ridge Dr. San Diego, CA 92121",
            "department": "None"
        },
        {
            "first_name": "Mark",
            "middle_name": "",
            "last_name": "Nowakowski",
            "name_suffix": "",
            "institution": "Mesa Tech International, Inc., 6404 Nancy Ridge Dr. San Diego, CA 92121",
            "department": "None"
        },
        {
            "first_name": "Courtney",
            "middle_name": "",
            "last_name": "Martin",
            "name_suffix": "",
            "institution": "Mesa Tech International, Inc., 6404 Nancy Ridge Dr. San Diego, CA 92121",
            "department": "None"
        },
        {
            "first_name": "YongPing",
            "middle_name": "",
            "last_name": "Duan",
            "name_suffix": "",
            "institution": "U. S. Horticultural Research Laboratory, 2001 South Rock Road, Fort Pierce, FL 34945",
            "department": "None"
        }
    ],
    "date_submitted": "2014-11-20T07:36:26+08:00",
    "date_accepted": "2014-11-20T07:36:26+08:00",
    "date_published": "2014-01-01T08:00:00+08:00",
    "render_galley": null,
    "galleys": [
        {
            "label": "",
            "type": "pdf",
            "path": "https://journalpub.escholarship.org/iocv_journalcitruspathology/article/41227/galley/30826/download/"
        }
    ]
}