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{
    "pk": 41300,
    "title": "Citrus tristeza virus-based RNA-interference (RNAi) vector and its potential in combating citrus Huanglongbing (HLB)",
    "subtitle": null,
    "abstract": "Citrus tristeza virus\n (CTV), a plus-sense ssRNA virus, is member of the genus Closterovirus, family \nClosteroviridae\n. RNA viruses are inducers as-well-as targets of gene silencing defense mechanism of host plants and this has been exploited as a tool in functional genomics. CTV was developed into virus-induced gene silencing (VIGS) or RNA-interference (RNAi) vector, which interferes with expression of endogenous genes in citrus or GFP-transgene in \nNicotiana benthamiana \n(16c) in a sequence specific manner. Photobleaching phenotype indicative of silencing of endogenous gene, phytoene desaturase in citrus, and red color under UV indicative of silencing of transgene GFP in \nN. benthamiana\n (16c) was observed using CTV-RNAi vector. CTV-RNAi vector has great potentials in combating huanglongbing (HLB) disease through (1) enhancing basal defense of citrus by silencing of auxin signaling F-Box receptor genes while simultaneously overexpressing microRNAs; (2) down-regulation of overexpressed genes, callose synthase and phloem protein-2, responsible for phloem-plugging in citrus by HLB; (3) expressing dsRNA specific to essential genes of insect vector psyllid (\nDiaphorina citri\n) to disable transmission of '\nCandidatus\n' Liberibacter asiaticus pathogen. Simultaneous silencing of multiple endogenous genes of a metabolic pathway is possible through tandem engineering of potential siRNA eliciting regions in CTV-RNAi vector.",
    "language": "en",
    "license": {
        "name": "Creative Commons Attribution 4.0",
        "short_name": "CC BY 4.0",
        "text": "Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.\n\nNo additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.",
        "url": "https://creativecommons.org/licenses/by/4.0"
    },
    "keywords": [],
    "section": "Abstracts of Presentations at the 3rd International Research Conference on Huanglongbing",
    "is_remote": true,
    "remote_url": "https://escholarship.org/uc/item/52d5x1fx",
    "frozenauthors": [
        {
            "first_name": "Shubash",
            "middle_name": "",
            "last_name": "Hajeri",
            "name_suffix": "",
            "institution": "Citrus Research and Education Center, University of Florida, 700 Experiment Station Road,\nLake Alfred, FL 33850 USA",
            "department": "None"
        },
        {
            "first_name": "Choaa",
            "middle_name": "",
            "last_name": "El-Mohtar",
            "name_suffix": "",
            "institution": "Citrus Research and Education Center, University of Florida, 700 Experiment Station Road,\nLake Alfred, FL 33850 USA",
            "department": "None"
        },
        {
            "first_name": "William",
            "middle_name": "O.",
            "last_name": "Dawson",
            "name_suffix": "",
            "institution": "Citrus Research and Education Center, University of Florida, 700 Experiment Station Road,\nLake Alfred, FL 33850 USA",
            "department": "None"
        },
        {
            "first_name": "Siddarame",
            "middle_name": "",
            "last_name": "Gowda",
            "name_suffix": "",
            "institution": "Citrus Research and Education Center, University of Florida, 700 Experiment Station Road,\nLake Alfred, FL 33850 USA",
            "department": "None"
        }
    ],
    "date_submitted": "2014-12-17T14:24:55-05:00",
    "date_accepted": "2014-12-17T14:24:55-05:00",
    "date_published": "2013-12-31T19:00:00-05:00",
    "render_galley": null,
    "galleys": [
        {
            "label": "",
            "type": "pdf",
            "path": "https://journalpub.escholarship.org/iocv_journalcitruspathology/article/41300/galley/30899/download/"
        }
    ]
}