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{
    "pk": 41333,
    "title": "Modulation of plant defense responses by Salicylate hydroxylase of Candidatus Liberibacter asiaticus",
    "subtitle": null,
    "abstract": "Citrus huanglongbing (HLB) associated with the pathogen \nCandidatus \nLiberibacter asiaticus (Las) is a devastating disease for the US citrus industry (1, 2). To gain knowledge on the mechanism(s) by which Las evades host defense responses, we first expressed salicylate hydroxylase (\nsahA\n) of Las\n \nin \nEscherichia coli. \nOur data indicate that Las encodes a functional salicylate hydroxylase, which converts salicylic acid (SA) into catechol, a product that does not induce resistance. The \nsahA\n gene was highly induced \nin planta \ncompared to psyllid vector suggesting its important role in disease progression. To determine expression level of defense related genes after Las infection, \nXanthomonas axonopodis \npv. \ncitri \nstrain AW (Xac AW) was used to induce \nPR \ngene expression. The \nPR-1 \ngene expression in Xac Aw-challenged plants, which were previously infected with Las, was lower than Xac Aw-challenged healthy plants. Using SA biosensor strain (\nAcinetobacter\n sp. ADPWH_lux), 4 fold reduction in SA accumulation was observed in the Las-infected as compared to healthy plants. To understanda possible synergistic effect of the presence of Las on the citrus canker [caused by \nX. citri\n subsp. \ncitri\n (Xcc)] we inoculated Xcc in Las-infected and healthy leaves of grapefruit. The population levels of Xcc were significantly higher during all the observation time points (up to 14 days) in Las-infected as compared to healthy citrus indicating that modulation of SA production and subsequent regulation of defense related genes such as \nPR-1 \ngene could be one of the mechanisms deployed by Las to evade plant defense responses. The Las-infected plants compromised with defense responses could further succumb to the infection by other pathogens. We also conducted experiments to restore the SA level in Las infected plants using SA hydroxylase inhibitors and test their effect in controlling HLB.",
    "language": "en",
    "license": {
        "name": "Creative Commons Attribution 4.0",
        "short_name": "CC BY 4.0",
        "text": "Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.\n\nNo additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.",
        "url": "https://creativecommons.org/licenses/by/4.0"
    },
    "keywords": [],
    "section": "Abstracts of Presentations at the 3rd International Research Conference on Huanglongbing",
    "is_remote": true,
    "remote_url": "https://escholarship.org/uc/item/4cs8f970",
    "frozenauthors": [
        {
            "first_name": "Pankaj",
            "middle_name": "",
            "last_name": "Trivedi",
            "name_suffix": "",
            "institution": "Citrus Research and Education Center, Department of Microbiology and Cell Science, University of Florida, 700 Experiment Station Road, Lake Alfred 33850, U.S.A.",
            "department": "None"
        },
        {
            "first_name": "Nian",
            "middle_name": "",
            "last_name": "Wang",
            "name_suffix": "",
            "institution": "Citrus Research and Education Center, Department of Microbiology and Cell Science, University of Florida, 700 Experiment Station Road, Lake Alfred 33850, U.S.A.",
            "department": "None"
        }
    ],
    "date_submitted": "2014-12-18T16:03:57-08:00",
    "date_accepted": "2014-12-18T16:03:57-08:00",
    "date_published": "2013-12-31T16:00:00-08:00",
    "render_galley": null,
    "galleys": [
        {
            "label": "",
            "type": "pdf",
            "path": "https://journalpub.escholarship.org/iocv_journalcitruspathology/article/41333/galley/30932/download/"
        }
    ]
}