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{
    "pk": 41377,
    "title": "Mandarin and mandarin hybrid genetic transformation for resistance to Candidatus Liberibacter asiaticus",
    "subtitle": null,
    "abstract": "Brazil is one of the largest producers and exporters of citrus. Currently, Huanglongbing disease (HLB) associated to \nCandidatus\n Liberibacter asiaticus (CLas) is the main threat to the citrus industry. The aim of this work is to study the genetic transformation of mandarin/mandarin hybrid 'Thomas' (\nCitrus reticulata\n Blanco) and 'Fremont' (\nC. clementina \nhort. ex Tanaka x\n C. reticulata \nBlanco) with the gene that encodes an attacin antibacterial peptide (\natt\nA) driven by phloem-specific promoters. The genetic transformation experiments were performed with epicotyl segments, via \nAgrobacterium tumefaciens\n (EHA 105), with the gene constructs pCAtSUC2/attA and pCAtPP2/attA, containing the \natt\nA gene controlled by AtSUC2 and AtPP2 promoters. Transgenic plants were identified by PCR analysis and acclimatized to greenhouse conditions. The plants will be propagated and evaluated for resistance to CLas.",
    "language": "en",
    "license": {
        "name": "Creative Commons Attribution 4.0",
        "short_name": "CC BY 4.0",
        "text": "Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.\n\nNo additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.",
        "url": "https://creativecommons.org/licenses/by/4.0"
    },
    "keywords": [],
    "section": "Abstracts of Presentations at the 3rd International Research Conference on Huanglongbing",
    "is_remote": true,
    "remote_url": "https://escholarship.org/uc/item/7gq5p7d7",
    "frozenauthors": [
        {
            "first_name": "L.",
            "middle_name": "",
            "last_name": "Soriano",
            "name_suffix": "",
            "institution": "Universidade de São Paulo/CENA, Piracicaba, Brazil",
            "department": "None"
        },
        {
            "first_name": "E.",
            "middle_name": "C.R.",
            "last_name": "Tavano",
            "name_suffix": "",
            "institution": "Universidade de São Paulo/CENA, Piracicaba, Brazil",
            "department": "None"
        },
        {
            "first_name": "R.",
            "middle_name": "",
            "last_name": "Harakava",
            "name_suffix": "",
            "institution": "Instituto Biológico, Secretaria de Agricultura e Abastecimento do Estado de São Paulo, São Paulo, Brazil",
            "department": "None"
        },
        {
            "first_name": "F.",
            "middle_name": "A.A.",
            "last_name": "Mourão Filho",
            "name_suffix": "",
            "institution": "Universidade de São Paulo/ESALQ, Piracicaba, Brazil",
            "department": "None"
        },
        {
            "first_name": "B.",
            "middle_name": "M.J.",
            "last_name": "Mendes",
            "name_suffix": "",
            "institution": "Universidade de São Paulo/CENA, Piracicaba, Brazil",
            "department": "None"
        }
    ],
    "date_submitted": "2014-12-23T14:22:12-05:00",
    "date_accepted": "2014-12-23T14:22:12-05:00",
    "date_published": "2013-12-31T19:00:00-05:00",
    "render_galley": null,
    "galleys": [
        {
            "label": "",
            "type": "pdf",
            "path": "https://journalpub.escholarship.org/iocv_journalcitruspathology/article/41377/galley/30976/download/"
        }
    ]
}