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{
    "pk": 41450,
    "title": "High throughput sequencing of a stem pitting citrus tristeza virus isolate from Hunan Province P.R. China",
    "subtitle": null,
    "abstract": "A stem-pitting isolate of citrus tristeza virus (CTV), spreading in Hunan province of  China (HU-PSTS), was sequenced and indexed on indicator plants. Biological assays showed that HU-PSTS is a highly aggressive stem pitting isolate belonging to biogroup 5. Viral small RNAs (18-26 nt) of the isolate were deep sequenced by Illumina technology to gain genomic information on the CTV strain infecting the source plant. The reads mapping with 17 CTV reference genomes enabled us to re-assemble the genomes of VT, T68, T30 and T3 strains. Among the VT, the highest number of mapped reads (47-41%) was with SG29, T318A, CT11A, Nuaga and AT-1 genomes, whereas T68, T30 and T3 genomes were less represented (28-20%). Alignments with genomes belonging to T36 and RB strains revealed percentage of mapped reads ranging from 10 to 12%. This is the first sequenced genome of a CTV isolate from Hunan province. According to the results, further sequencing and bioindexing need to be developed to explore the potential of a local cross protective strategy.",
    "language": "en",
    "license": {
        "name": "Creative Commons Attribution 4.0",
        "short_name": "CC BY 4.0",
        "text": "Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.\n\nNo additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.",
        "url": "https://creativecommons.org/licenses/by/4.0"
    },
    "keywords": [
        {
            "word": "Citrus tristeza virus"
        },
        {
            "word": "next generation sequencing"
        },
        {
            "word": "small RNAs"
        },
        {
            "word": "reference genomes."
        }
    ],
    "section": "Recently Accepted",
    "is_remote": true,
    "remote_url": "https://escholarship.org/uc/item/99g3b9vw",
    "frozenauthors": [
        {
            "first_name": "Grazia",
            "middle_name": "",
            "last_name": "Licciardello",
            "name_suffix": "",
            "institution": "Science and Technology Park of Sicily, z.i. Blocco Palma I, Stradale Lancia 57, 95121 Catania, Italy\n\nAgrobiotech Soc. Coop. z.i. Blocco Palma I, Stradale Lancia 57, 95121 Catania, Italy",
            "department": "None"
        },
        {
            "first_name": "Giuseppe",
            "middle_name": "",
            "last_name": "Scuderi",
            "name_suffix": "",
            "institution": "Science and Technology Park of Sicily, z.i. Blocco Palma I, Stradale Lancia 57, 95121 Catania, Italy\n\nAgrobiotech Soc. Coop. z.i. Blocco Palma I, Stradale Lancia 57, 95121 Catania, Italy",
            "department": "None"
        },
        {
            "first_name": "Rosario",
            "middle_name": "",
            "last_name": "Ferraro",
            "name_suffix": "",
            "institution": "Science and Technology Park of Sicily, z.i. Blocco Palma I, Stradale Lancia 57, 95121 Catania, Italy",
            "department": "None"
        },
        {
            "first_name": "Marcella",
            "middle_name": "",
            "last_name": "Russo",
            "name_suffix": "",
            "institution": "Science and Technology Park of Sicily, z.i. Blocco Palma I, Stradale Lancia 57, 95121 Catania, Italy\n\nAgrobiotech Soc. Coop. z.i. Blocco Palma I, Stradale Lancia 57, 95121 Catania, Italy",
            "department": "None"
        },
        {
            "first_name": "Su Ming",
            "middle_name": "",
            "last_name": "Dai",
            "name_suffix": "",
            "institution": "National Center for Citrus Improvement (Changsha), Hunan Agricultural University, Hunan 410128, P.R. China.",
            "department": "None"
        },
        {
            "first_name": "Antonino",
            "middle_name": "F.",
            "last_name": "Catara",
            "name_suffix": "",
            "institution": "Science and Technology Park of Sicily, z.i. Blocco Palma I, Stradale Lancia 57, 95121 Catania, Italy",
            "department": "None"
        },
        {
            "first_name": "Ziniu",
            "middle_name": "N",
            "last_name": "Deng",
            "name_suffix": "",
            "institution": "National Center for Citrus Improvement (Changsha), Hunan Agricultural University, Hunan 410128, P.R. China.",
            "department": "None"
        }
    ],
    "date_submitted": "2020-11-09T19:30:26+09:00",
    "date_accepted": "2020-11-09T19:30:26+09:00",
    "date_published": "2023-09-07T16:00:00+09:00",
    "render_galley": null,
    "galleys": [
        {
            "label": "",
            "type": "pdf",
            "path": "https://journalpub.escholarship.org/iocv_journalcitruspathology/article/41450/galley/31031/download/"
        }
    ]
}