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{ "pk": 41471, "title": "Two distinct viral suppressors of RNA silencing encoded by citrus tatter leaf virus", "subtitle": null, "abstract": "Two proteins of the citrus tatter leaf virus (CTLV), a strain of the apple stem grooving virus (ASGV), capable of inducing citrus bud union disorders on commercially important trifoliate and citrange rootstocks, were identified as viral suppressors of RNA silencing (VSR). Both the coat protein (CP) and the movement protein (MP) suppressed RNA silencing in GFP-transgenic \nNicotiana benthamiana\n 16c plants in agrobacterium-mediated co-infiltration assays; the MP acted as a local VSR, while the CP acted as a systemic VSR. When the potato virus X (PVX) infectious vector harbored either the CTLV CP or MP gene, viral infection and symptom development were promoted in \nN. benthamiana\n. Deletions of amino acids in the CP sequence or the MP sequence resulted in failure to promote PVX infections as well as suppression of silencing in agrobacterium-mediated co-infiltration assays. Mass spectrometry-based immunoprecipitation proteomics showed that neither the CTLV CP nor the MP interacts with cellular components directly involved in host antiviral RNA silencing pathways. RNA immunoprecipitation (RIP) and RNA-protein pull-down assays indicated that the CTLV MP interacts with double-stranded RNA (dsRNA) presumably through a protein complex or proteins containing RNA binding domains. It is possible that the MP prevents dsRNA cleavage through this mechanism, leading to suppression of host antiviral RNA silencing. These findings confirmed that CTLV uses VSRs as part of its overall strategy to overcome host antiviral defenses and are indicative of the ability of ASGV and CTLV to infect a wide range of hosts including different species of woody and herbaceous plants.", "language": "en", "license": { "name": "Creative Commons Attribution 4.0", "short_name": "CC BY 4.0", "text": "Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.\n\nNo additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.", "url": "https://creativecommons.org/licenses/by/4.0" }, "keywords": [ { "word": "VSR" }, { "word": "Capillovirus" }, { "word": "post-transcriptional gene silencing" }, { "word": "coat protein (CP)" }, { "word": "movement protein (MP)" }, { "word": "apple stem grooving virus (ASGV)" } ], "section": "Articles", "is_remote": true, "remote_url": "https://escholarship.org/uc/item/30p6r1pd", "frozenauthors": [ { "first_name": "Shih-hua", "middle_name": "", "last_name": "Tan", "name_suffix": "", "institution": "Department of Microbiology and Plant Pathology, University of California, Riverside, California, United States of America, 92521", "department": "None" }, { "first_name": "Sohrab", "middle_name": "", "last_name": "Bodaghi", "name_suffix": "", "institution": "Department of Microbiology and Plant Pathology, University of California, Riverside, California, United States of America, 92521", "department": "None" }, { "first_name": "Arunabha", "middle_name": "", "last_name": "Mitra", "name_suffix": "", "institution": "Department of Microbiology and Plant Pathology, University of California, Riverside, California, United States of America, 92521", "department": "None" }, { "first_name": "Stacey", "middle_name": "", "last_name": "Comstock", "name_suffix": "", "institution": "Department of Microbiology and Plant Pathology, University of California, Riverside, California, United States of America, 92521", "department": "None" }, { "first_name": "Amy", "middle_name": "", "last_name": "Huang", "name_suffix": "", "institution": "Department of Microbiology and Plant Pathology, University of California, Riverside, California, United States of America, 92521", "department": "None" }, { "first_name": "Sarah", "middle_name": "", "last_name": "Hammado", "name_suffix": "", "institution": "Department of Microbiology and Plant Pathology, University of California, Riverside, California, United States of America, 92521", "department": "None" }, { "first_name": "Jinliang", "middle_name": "", "last_name": "Liu", "name_suffix": "", "institution": "College of Plant Sciences, Jilin University, Changchun, China, 130062", "department": "None" }, { "first_name": "Shurooq", "middle_name": "", "last_name": "Abu-Hajar", "name_suffix": "", "institution": "Department of Microbiology and Plant Pathology, University of California, Riverside, California, United States of America, 92521", "department": "None" }, { "first_name": "Paulina", "middle_name": "", "last_name": "Quijia-Lamina", "name_suffix": "", "institution": "Department of Microbiology and Plant Pathology, University of California, Riverside, California, United States of America, 92521", "department": "None" }, { "first_name": "German", "middle_name": "Rafael", "last_name": "Villalba-Salazar", "name_suffix": "", "institution": "Department of Microbiology and Plant Pathology, University of California, Riverside, California, United States of America, 92521", "department": "None" }, { "first_name": "Greg", "middle_name": "W.", "last_name": "Douhan", "name_suffix": "", "institution": "Department of Microbiology and Plant Pathology, University of California, Riverside, California, United States of America, 92521", "department": "None" }, { "first_name": "Irene", "middle_name": "", "last_name": "Lavagi-Craddock", "name_suffix": "", "institution": "Department of Microbiology and Plant Pathology, University of California, Riverside, California, United States of America, 92521", "department": "None" }, { "first_name": "Abigail", "middle_name": "Marie", "last_name": "Frolli", "name_suffix": "", "institution": "University of California Agriculture and Natural Resources, Lindcove Research and Extension Center, 22963 Carson Avenue, Exeter, CA 93221", "department": "None" }, { "first_name": "Ashraf", "middle_name": "", "last_name": "El-Kereamy", "name_suffix": "", "institution": "Department of Botany and Plant Sciences, University of California, Riverside, California, United States of America, 92521", "department": "None" }, { "first_name": "Georgios", "middle_name": "", "last_name": "Vidalakis", "name_suffix": "", "institution": "Department of Microbiology and Plant Pathology, University of California, Riverside, California, United States of America, 92521", "department": "None" } ], "date_submitted": "2023-12-14T15:23:42-03:00", "date_accepted": "2023-12-14T15:23:42-03:00", "date_published": "2024-08-26T03:00:00-04:00", "render_galley": null, "galleys": [ { "label": "", "type": "pdf", "path": "https://journalpub.escholarship.org/iocv_journalcitruspathology/article/41471/galley/31047/download/" } ] }