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{
    "pk": 16594,
    "title": "Prevalence of Bordetella pertussis and Bordetella parapertussis in Samples Submitted for RSV Screening",
    "subtitle": null,
    "abstract": "Background: The clinical presentation of Bordetella pertussis can overlap with that of respiratory syncytial virus (RSV); however, management differs.\n\n\nHypothesis: First, the prevalence of B. pertussis is less than 2% among patients screened for RSV, and second the prevalence of B. parapertussis is also less than 2% among these patients.\n\n\nMethods: Nasal washings submitted to a clinical laboratory for RSV screening were tested for B. pertussis and B. parapertussis, using species-specific real-time polymerase chain reaction (PCR) assays. These were optimized to target conserved regions within a complement gene and the CarB gene, respectively. A Bordetella spp. genus-specific real-time PCR assay was designed to detect the Bhur gene of B. pertussis, B. parapertussis, and B. bronchiseptica. RSV A and B subtypes were tested by reverse transcription-PCR.\n\n\nResults: Four hundred and eighty-nine clinical samples were tested. There was insufficient material to complete testing for one B. pertussis, 10 RSV subtype A, and four RSV subtype B assays. Bordetella pertussis was detected in 3/488 (0.6%) (95% CI 0.1% to 1.8%), while B. parapertussis was detected in 5/489 (1.0%) (95% CI 0.3% to 2.4%). Dual infection of B. pertussis with RSV and of B. parapertussis with RSV occurred in two and in three cases respectively. RSV was detected by PCR in 127 (26.5%).\n\n\nConclusion: The prevalence of B. pertussis in nasal washings submitted for RSV screening was less than 2%. The prevalence of parapertussis may be higher than 2%. RSV with B. pertussis and RSV with B. parapertussis coinfection do occur.\n\n\n[WestJEM. 2008;9:135-140.]",
    "language": "en",
    "license": {
        "name": "none",
        "short_name": "none",
        "text": "",
        "url": "http://google.com"
    },
    "keywords": [
        {
            "word": "Bordetella pertussis"
        },
        {
            "word": "Bordetella parpertussis"
        },
        {
            "word": "Respiratory Syncytial Virus"
        }
    ],
    "section": "Article",
    "is_remote": true,
    "remote_url": "https://escholarship.org/uc/item/1j23x5qb",
    "frozenauthors": [
        {
            "first_name": "Paul",
            "middle_name": "",
            "last_name": "Walsh",
            "name_suffix": "",
            "institution": "Department of Emergency Medicine, Kern Medical Center, Bakersfield, CA",
            "department": "None"
        },
        {
            "first_name": "Christina",
            "middle_name": "",
            "last_name": "Lim-Overmeyer",
            "name_suffix": "",
            "institution": "Research and Development Department, Medical Diagnostic Laboratories, Hamilton, NJ",
            "department": "None"
        },
        {
            "first_name": "Lauren",
            "middle_name": "",
            "last_name": "Kimmel",
            "name_suffix": "",
            "institution": "Research and Development Department, Medical Diagnostic Laboratories, Hamilton ,NJ",
            "department": "None"
        },
        {
            "first_name": "Melanie",
            "middle_name": "",
            "last_name": "Feola",
            "name_suffix": "",
            "institution": "Research and Development Department, Medical Diagnostic Laboratories, Hamilton ,NJ",
            "department": "None"
        },
        {
            "first_name": "James",
            "middle_name": "",
            "last_name": "Pusavaat",
            "name_suffix": "",
            "institution": "Department of Pathology, Kern Medical Center, Bakersfield ,CA",
            "department": "None"
        },
        {
            "first_name": "Tuan Anh",
            "middle_name": "",
            "last_name": "Nguyen",
            "name_suffix": "",
            "institution": "Department of Emergency Medicine, Kern Medical Center, Bakersfield ,CA",
            "department": "None"
        },
        {
            "first_name": "Sam",
            "middle_name": "",
            "last_name": "Kuan",
            "name_suffix": "",
            "institution": "Department of Emergency Medicine, Kern Medical Center, Bakersfield CA.",
            "department": "None"
        },
        {
            "first_name": "Kirt",
            "middle_name": "",
            "last_name": "Emery",
            "name_suffix": "",
            "institution": "Kern County Department of Public Health",
            "department": "None"
        },
        {
            "first_name": "Martin",
            "middle_name": "",
            "last_name": "Rosengreen",
            "name_suffix": "",
            "institution": "Department of Emergency Medicine, Kern Medical Center, Bakersfield ,CA",
            "department": "None"
        },
        {
            "first_name": "Eli",
            "middle_name": "",
            "last_name": "Mordechai",
            "name_suffix": "",
            "institution": "Research and Development Department, Medical Diagnostic Laboratories, Hamilton ,NJ",
            "department": "None"
        },
        {
            "first_name": "Martin",
            "middle_name": "E",
            "last_name": "Adelson",
            "name_suffix": "",
            "institution": "Research and Development Department, Medical Diagnostic Laboratories, Hamilton ,NJ",
            "department": "None"
        }
    ],
    "date_submitted": "2007-11-12T08:00:00Z",
    "date_accepted": "2007-11-12T08:00:00Z",
    "date_published": "2008-06-23T07:00:00Z",
    "render_galley": null,
    "galleys": [
        {
            "label": "",
            "type": "pdf",
            "path": "https://journalpub.escholarship.org/westjem/article/16594/galley/8394/download/"
        }
    ]
}