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{
    "pk": 59131,
    "title": "Decelluarization and Reseeding of Porcine Colon with Human CaCo-2 Cells for Whole-Scale Organ Engineering Applications",
    "subtitle": null,
    "abstract": "A new approach to whole-scale organ engineering is the seeding of stem cells upon a decellularized scaffold of a desired organ. Current efforts seek to use this method for whole-scale organ engineering. Here, we report a novel decellularizationrecellularization approach to engineering a human colonic graft with human enterocytes and xenogeneic scaffold. Porcine colon tissue was decellularized with protein-solubilizing agents and recellularized by incubation with Caco-2. Immunohistochemistry, DNA staining, and SEM were used to study the effects upon the scaffold. Exposure to 2%SDS-0.5%Triton solution for four hours resulted in the best decellularization and preservation of microstructure, while tissues treated with 2%SDS-0.5%Triton and 0.05%Trypsin/EDTA supported the best recellularization. Our method provides a potential strategy for streamlined regeneration of a viable colonic lumen from xenogeneic and allogeneic sources while maintaining colon microstructure and potential for recellularization.",
    "language": "en",
    "license": null,
    "keywords": [],
    "section": "Research",
    "is_remote": true,
    "remote_url": "https://escholarship.org/uc/item/3b0743s3",
    "frozenauthors": [
        {
            "first_name": "Julie",
            "middle_name": "",
            "last_name": "Haduong",
            "name_suffix": "",
            "institution": "University of California, Berkeley",
            "department": ""
        },
        {
            "first_name": "Rahul",
            "middle_name": "",
            "last_name": "Nayak",
            "name_suffix": "",
            "institution": "University of California, Berkeley",
            "department": ""
        },
        {
            "first_name": "Yumi",
            "middle_name": "",
            "last_name": "Suh",
            "name_suffix": "",
            "institution": "University of California, Berkeley",
            "department": ""
        },
        {
            "first_name": "Vinay",
            "middle_name": "",
            "last_name": "Viswanadham",
            "name_suffix": "",
            "institution": "University of California, Berkeley",
            "department": ""
        }
    ],
    "date_submitted": "2014-12-18T08:28:29Z",
    "date_accepted": "2014-12-18T08:28:29Z",
    "date_published": "2014-12-18T08:00:00Z",
    "render_galley": null,
    "galleys": [
        {
            "label": "",
            "type": "pdf",
            "path": "https://journalpub.escholarship.org/our_bsj/article/59131/galley/45152/download/"
        }
    ]
}